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肾损伤分子1(Kim1)检测试剂盒(化学发光免疫分析法)

CLIA Kit for Kidney Injury Molecule 1 (Kim1)

HAVCR1; TIM1; TIMD1; HAVCR; Hepatitis A Virus Cellular Receptor 1; T Cell Immunoglobulin And Mucin Domain-Containing Protein 1; T-cell immunoglobulin mucin receptor 1

  • 肾损伤分子1(Kim1)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 肾损伤分子1(Kim1)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 肾损伤分子1(Kim1)检测试剂盒(化学发光免疫分析法) 实验结果图
  • SCA785Mu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测肾损伤分子1(Kim1),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100μL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加底物100µL,37°C孵育10分钟;
8. 读数。

实验原理

将肾损伤分子1(Kim1)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的肾损伤分子1(Kim1)与连接于固相载体上的抗体结合,然后加入生物素化的肾损伤分子1(Kim1)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的肾损伤分子1(Kim1)呈正相关。用化学发光仪测定相对光单位(RLU),计算样品浓度。

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
RPA785Mu01 肾损伤分子1(Kim1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA785Mu02 肾损伤分子1(Kim1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA785Mu61 肾损伤分子1(Kim1)活性蛋白 Cell culture; Activity Assays.
EPA785Mu61 肾损伤分子1(Kim1)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA785Mu01 肾损伤分子1(Kim1)多克隆抗体 WB; IHC; ICC; IP.
PAA785Mu02 肾损伤分子1(Kim1)多克隆抗体 WB; IHC; ICC; IP.
LAA785Mu81 肾损伤分子1(Kim1)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA785Mu71 肾损伤分子1(Kim1)多克隆抗体(生物素标记) WB; IHC; ICC.
SEA785Mu 肾损伤分子1(Kim1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA785Mu 肾损伤分子1(Kim1)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA785Mu 肾损伤分子1(Kim1)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA785Mu01 肾损伤分子1(Kim1)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Clinical Toxicology Clinical implication of urinary neutrophil gelatinase-associated lipocalin and kidney injury molecule-1 in patients with acute paraquat intoxication [PubMed: 19827907]
Pediatric Nephrology KIM-1 and NGAL: new markers of obstructive nephropathy [SpringerLink: v461592787w5488x]
Pediatric Research Urinary Neutrophil Gelatinase-Associated Lipocalin at Birth Predicts Early Renal Function in Very Low Birth Weight Infants [LWW: 10000]
Archives of Medical Science Markers of kidney function in the elderly in relation to the new CKD-EPI formula for estimation of glomerular filtration rate [AMS: source]
Nephrology Targeting the AGE-RAGE axis improves renal function in the context of a healthy diet low in advanced glycation end-product content [PubMed: 23046363]
Nanotoxicology. Comprehensive assessment of nephrotoxicity of intravenously administered sodium-oleate-coated ultra-small superparamagnetic iron oxide (USPIO) and titanium dioxide (TiO(2)) nanoparticles in rats. [PubMed: 23272807]
Toxicological Science A Mouse Diversity Panel Approach Reveals the Potential for Clinical Kidney Injury Due to DB289 Not Predicted by Classical Rodent Models [PubMed: PMC3498743]
Disease Markers Interleukin 18 as a Marker of Chronic Nephropathy in Children after Anticancer Treatment [Hindawi: 369784]
Journal of Biomarkers Urinary Measurement of Neutrophil Gelatinase Associated Lipocalin and Kidney Injury Molecule-1 Helps Diagnose Acute Pyelonephritis in a Preclinical Model [Hindawi: 413853]
Advances in Life Science and Technology Kidney injury molecule-1: a urinary biomarker for contrast induced acute kidney injury. [Iiste: Source]
Nephrology Urinary neutrophil gelatinase-associated lipocalin predicts graft loss after acute kidney injury in kidney transplant [Pubmed: 24325180]
Nephrology Targeting the AGE-RAGE axis improves renal function in the context of a healthy diet low in advanced glycation end-product content. [Pubmed: 23046363]
BioMed Research International The Renal Effects of Vanadate Exposure: Potential Biomarkers and Oxidative Stress as a Mechanism of Functional Renal Disorders—Preliminary Studies [Hindawi: 740105]
Life Sci. Attenuation of renal ischemia/reperfusion injury by a?aí extract preconditioning in a rat model [Pubmed:25476829]
Diabetes & Vascular Disease Research Urinary Kim-1 is a sensitive biomarker for the early stage of diabetic nephropathy in Otsuka Long-Evans Tokushima Fatty rats [Sagepub:Source]
临床儿科杂志 中性粒细胞明胶酶相关脂质运载蛋白、肾损伤分子1和白介素18 在诊断心肺分流术后急性肾损伤中的意义 [Jcp-Sh:Source]
Nephrology (Carlton). Tubular urinary biomarkers do not identify aetiology of acute kidney injury in kidney transplant recipients [Pubmed:24666430]
PLoS One. Cilostazol Renoprotective Effect: Modulation of PPAR-γ, NGAL, KIM-1 and IL-18 Underlies Its Novel Effect in a Model of Ischemia-Reperfusion [Pubmed:24816434]
BMC Nephrology SYNbiotics Easing Renal failure by improving Gut microbiologY (SYNERGY): a protocol of placebo-controlled randomised cross-over trial [Pubmed:24996842]
Pediatric Nephrology The diagnosis of febrile urinary tract infection in children may be facilitated by urinary biomarkers [Pubmed:25127917]
Inflammation.? Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis [Pubmed:25448261]
Transworld Medical Journal Kidney injury molecule-1: A urinary biomarker for contrast-induced acute kidney injury [Tmjournals:Source]
Int J Clin Exp Pathol Rosiglitazone attenuates renal injury caused by hyperlipidemic pancreatitis [PubMed: 26191125]
Int J Clin Exp Pathol Inhalation of hydrogen gas ameliorates glyoxylate-induced calcium oxalate deposition and renal oxidative stress in mice [PubMed: 26045773]
Mol Med Rep Vorinostat protects against calcium oxalate-induced kidney injury in mice [PubMed: 26095064]
Pediatr Nephrol Role of new biomarkers for predicting renal scarring in vesicoureteral reflux: NGAL, KIM-1, and L-FABP [PubMed: 26324091]
Journal of Pediatric Urology Urinary NGAL, KIM-1 and L-FABP concentrations in antenatal hydronephrosis [PubMed: 26096437]
Am J Physiol Renal Physiol Intrarenal Renin-Angiotensin System Mediates Fatty Acid-Induced ER Stress in the Kidney [PubMed: 26672616]
Mol Med Rep. Attenuation of renal ischemia/reperfusion injury by oleanolic acid preconditioning via its antioxidant, anti‑inflammatory, and anti‑apoptotic activities. [Pubmed:27082705]
Nephrol. Dial. Transplant. Preconditioned suppression of prolyl-hydroxylases attenuates renal injury but increases mortality in septic murine models [Pubmed:26908768]
J Clin Lab Anal.  Kidney Injury Molecule Levels in Type 2 Diabetes Mellitus [Pubmed:27132791]
Med Sci Monit. Protective Effect of Dihydromyricetin Against Lipopolysaccharide-Induced Acute Kidney Injury in a Rat Model [pmc:PMC4754091]
Diabetes. Deficiency in Apoptosis-Inducing Factor Recapitulates Chronic Kidney Disease via Aberrant Mitochondrial Homeostasis [Pubmed:26822084]
Sci Rep. Once daily administration of the SGLT2 inhibitor, empagliflozin, attenuates markers of renal fibrosis without improving albuminuria in diabetic db/db mice [Pubmed:27226136]
Journal of Applied Life Sciences International Vitamins C and E Alleviate Nephrotoxicity-induced by Potassium Bromate in Rats [Barakat812016JALSI28698.pdf]
Oxidative Medicine and Cellular Longevity Synergic Effect of α-Mangostin on the Cytotoxicity of Cisplatin in a Cervical Cancer Model. [pubmed:28053694]
Molecular Medicine Reports Attenuation of renal ischemia/reperfusion injury by oleanolic acid preconditioning via itsantioxidant, anti‑inflammatory, and anti‑apoptotic activities. [pubmed:27082705]
Journal of Agricultural and Food Chemistry Protective Effects of DHA-PC against Vancomycin-Induced Nephrotoxicity through the Inhibition of Oxidative Stress and Apoptosis in BALB/c Mice [pubmed:29254330]
Clin Exp Pharmacol Physiol. Early urinary biomarkers of renal tubular damage by a high-salt intake independent of blood pressure in normotensive rats. [pubmed:29027259]
Nephron - Physiology Urinary Kidney Injury Molecule-1 but Not Urinary Neutrophil Gelatinase Associated Lipocalin Is Increased after Short Maximal Exercise [10.1159/000481179]
Cellular Physiology and Biochemistry Gum Acacia Improves Renal Function and Ameliorates Systemic Inflammation, Oxidative and Nitrosative Stress in Streptozotocin-Induced Diabetes in Rats … [Pubmed:29550811]
Frontiers in Pharmacology Exploring the therapeutic mechanism of Desmodium styracifolium on oxalate crystal-induced kidney injuries using comprehensive approaches based on … [Pubmed:29950996]
Molecules Effect of Pinocembrin Isolated from Mexican Brown Propolis on Diabetic Nephropathy [Pubmed:29642511]
American Journal of Physiology-Regulatory Integrative and Comparative Physiology Modeling heart failure risk in diabetes and kidney disease: limitations and potential applications of transverse aortic constriction in high-fat-fed mice [Pubmed:29443547]
Biological Trace Element Research Effect of Aqueous Extract of Glycyrrhiza glabra on the Biochemical Changes Induced by Cadmium Chloride in Rats [Pubmed: 30251228]
Cancer Research and Treatment A Phage Display-Identified Peptide Selectively Binds to Kidney Injury Molecule-1(KIM-1) and Detects KIM-1-Overexpressing Tumors in vivo []
Archives of Medical Science Urinary neutrophil gelatinase-associated lipocalin, kidney injury molecule-1, N-acetyl-β-D-glucosaminidase levels and mortality risk in septic patients with … [Pubmed: 30393493]
Biological & Pharmaceutical Bulletin UPLC/MS-Based Metabolomics Investigation of the Protective Effect of Hydrogen Gas Inhalation on Mice with Calcium Oxalate-Induced Renal Injury [Pubmed: 30381664]
Kidney and Blood Pressure Research The Kidney Specific Protein myo-Inositol Oxygenase, a Potential Biomarker for Diabetic Nephropathy [Pubmed: 30504713]
Clinica Chimica Acta Urine markers of renal tubular injury in idiopathic membranous nephropathy: A cross sectional study [Pubmed: 30684459]
BMC Complementary and Alternative Medicine Dual actions on gout flare and acute kidney injury along with enhanced renal transporter activities by Yokuininto, a Kampo medicine [Pubmed: 30871515]
2.583 Application of ELISA Technique and Human Microsomes in the Search for 11β-Hydroxysteroid Dehydrogenase Inhibitors [Doi: BioMed Research International]
Naunyn-Schmiedeberg's archives of pharmacology Piceatannol protects against cisplatin nephrotoxicity via activation of Nrf2/HO-1 pathway and hindering NF-κB inflammatory cascade [Pubmed: 31197431]
Egyptian Journal of Zoology Effect of Cucumis melo var. flexuosus leaves extract on renal oxidative injury and inflammation in diabetic male albino rats []
Environment International Toxicity assessment due to prenatal and lactational exposure to lead, cadmium and mercury mixtures [Pubmed: 31639605]
Bioscience Reports page Metabolomics study of fasudil on cisplatin-induced kidney injury [Pubmed: 31670380]
life sciences MiR-214-3p exacerbates kidney damages and inflammation induced by hyperlipidemic pancreatitis complicated with acute renal injury [Pubmed: 31790686]
Arch Physiol Biochem Protective effect of saxagliptin against renal ischaemia reperfusion injury in rats [Pubmed: 31979992]
clinical science Delineating a role for the Mitochondrial Permeability Transition Pore in Diabetic Kidney Disease by targeting Cyclophilin D [Pubmed: 31943002]
LIFE SCIENCES Rapamycin improves renal injury induced by Iodixanol in diabetic rats by deactivating the mTOR/p70S6K signaling pathway [Pubmed: 32798557]
INTERNATIONAL UROLOGY AND NEPHROLOGY Serum NGAL, KIM-1, IL-18, L-FABP: new biomarkers in the diagnostics of acute kidney injury (AKI) following invasive cardiology procedures [Pubmed: 32557377]
Journal of Pediatric Urology Can Serum Neutrophil Gelatinase Associated Lipocalin and Kidney Injury Molecule-1 Help in Decision Making for Surgery in Antenatally Dedected … [Pubmed: 33139211]
Ratlarda sodyum florid ile oluşturulan toksikasyonda Karvakol'ün böbrekler üzerine etkisi []
Rationale and design of the intervention in PHOspholamban RElated CArdiomyopathy STudy (i-PHORECAST) []
Pediatr Int Early Kidney Injury in Immunoglobulin A Vasculitis: Role of Renal Biomarkers [33423326]
J Int Med Res Value of urinary kidney injury molecule-1 levels in predicting acute kidney injury in very low birth weight preterm infants [33372811]
Biomed Res Int Renal Protective Effect of Beluga Lentil Pretreatment for Ischemia-Reperfusion Injury [33604384]
Nutrients Targeting Methylglyoxal in Diabetic Kidney Disease Using the Mitochondria-Targeted Compound MitoGamide [33922959]
J Am Soc Nephrol Kim-1 Targeted Extracellular Vesicles: A New Therapeutic Platform for RNAi to Treat AKI [34127536]
Heliyon Anti-oxidant impact of Lisinopril and Enalapril against acute kidney injury induced by doxorubicin in male Wistar rats: involvement of kidney injury molecule-1 [33506137]
Journal of Pharmacological Sciences Pentoxifylline treatment alleviates kidney ischemia/reperfusion injury: Novel involvement of galectin-3 and ASK-1/JNK & ERK1/2/NF-¦ÊB/HMGB-1 trajectories [34030796]
Int Urol Nephrol Could urinary kidney injury molecule-1 be a good marker in subclinical acute kidney injury in mild to moderate COVID-19 infection? [34213713]
Intensive Care Medicine Experimental Preservation of renal endothelial integrity and reduction of renal edema by aprotinin does not preserve renal perfusion and function following experimental … [34169407]
iscience Kidney disease risk factors do not explain impacts of low dietary protein on kidney function and structure [34820603]
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