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神经生长因子(NGF)检测试剂盒(化学发光免疫分析法)

CLIA Kit for Nerve Growth Factor (NGF)

NGFB; Beta-NGF; HSAN5; NGF-B; Beta-Nerve Growth Factor

  • 神经生长因子(NGF)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 神经生长因子(NGF)检测试剂盒(化学发光免疫分析法) 产品包装(模拟)
  • 神经生长因子(NGF)检测试剂盒(化学发光免疫分析法) 实验结果图
  • SCA105Hu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测神经生长因子(NGF),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的神经生长因子(NGF)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 82-104 88
EDTA plasma(n=5) 93-101 96
heparin plasma(n=5) 79-101 89

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的神经生长因子(NGF),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中神经生长因子(NGF)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 81-93% 84-101% 90-101% 91-101%
EDTA plasma(n=5) 84-99% 93-105% 79-89% 84-103%
heparin plasma(n=5) 89-98% 80-88% 97-105% 86-93%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100μL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加底物100µL,37°C孵育10分钟;
8. 读数。

实验原理

将神经生长因子(NGF)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的神经生长因子(NGF)与连接于固相载体上的抗体结合,然后加入生物素化的神经生长因子(NGF)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的神经生长因子(NGF)呈正相关。用化学发光仪测定相对光单位(RLU),计算样品浓度。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA105Hu02 神经生长因子(NGF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA105Hu61 神经生长因子(NGF)活性蛋白 Cell culture; Activity Assays.
RPA105Hu01 神经生长因子(NGF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA105Hu01 神经生长因子(NGF)活性蛋白 Cell culture; Activity Assays.
EPA105Hu61 神经生长因子(NGF)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA105Hu02 神经生长因子(NGF)多克隆抗体 WB; IHC; ICC; IP.
PAA105Hu01 神经生长因子(NGF)多克隆抗体 WB; IHC; ICC; IP.
PAA105Hu06 神经生长因子(NGF)多克隆抗体 WB; IHC; ICC; IP.
LAA105Hu71 神经生长因子(NGF)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA105Hu25 神经生长因子(NGF)单克隆抗体 WB; IHC; ICC; IP.
MAA105Hu27 神经生长因子(NGF)单克隆抗体 WB; IHC; ICC; IP.
MAA105Hu29 神经生长因子(NGF)单克隆抗体 IHC
MAA105Hu21 神经生长因子(NGF)单克隆抗体 WB
MAA105Hu22 神经生长因子(NGF)单克隆抗体 WB,ICC/IF
MAA105Hu24 神经生长因子(NGF)单克隆抗体 WB; IHC; ICC; IP.
MAA105Hu23 神经生长因子(NGF)单克隆抗体 WB
FAA105Hu02 抗神经生长因子(NGF)单克隆抗体 Flow cytometry.
SEA105Hu 神经生长因子(NGF)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA105Hu 神经生长因子(NGF)检测试剂盒(酶联免疫吸附试验法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA105Hu 神经生长因子(NGF)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA105Hu 神经生长因子(NGF)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA105Hu01 神经生长因子(NGF)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

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