Ⅰ型前胶原羧基端原肽(PICP)检测试剂盒(化学发光免疫分析法)
CLIA Kit for Procollagen I C-Terminal Propeptide (PICP)
P1CP; C-Propeptide Of Type I Procollagen; Procollagen I Carboxy Terminal Propeptide
- 编号SCA570Hu
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长2h, 40min
- 检测范围61.73-5,000pg/mL
- 灵敏度最小可检测剂量小于等于25.18pg/mL.
- 样本类型Serum, plasma and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 3528 ¥ 5040 ¥ 22680 ¥ 42840 ¥ 352800
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测Ⅰ型前胶原羧基端原肽(PICP)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的Ⅰ型前胶原羧基端原肽(PICP)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 91-101 | 94 |
| EDTA plasma(n=5) | 79-101 | 96 |
| heparin plasma(n=5) | 88-96 | 92 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的Ⅰ型前胶原羧基端原肽(PICP)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中Ⅰ型前胶原羧基端原肽(PICP)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 83-101% | 88-97% | 85-92% | 93-101% |
| EDTA plasma(n=5) | 89-97% | 84-97% | 80-105% | 92-105% |
| heparin plasma(n=5) | 83-97% | 96-103% | 85-94% | 84-91% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100μL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加底物100µL，37°C孵育10分钟；
8. 读数。
实验原理
将Ⅰ型前胶原羧基端原肽(PICP)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的Ⅰ型前胶原羧基端原肽(PICP)与连接于固相载体上的抗体结合，然后加入生物素化的Ⅰ型前胶原羧基端原肽(PICP)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的Ⅰ型前胶原羧基端原肽(PICP)呈正相关。用化学发光仪测定相对光单位（RLU），计算样品浓度。
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA570Hu01 | Ⅰ型前胶原羧基端原肽(PICP)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA570Hu61 | Ⅰ型前胶原羧基端原肽(PICP)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA570Hu61 | Ⅰ型前胶原羧基端原肽(PICP)活性蛋白 | Cell culture; Activity Assays. |
| CPA570Hu22 | Ⅰ型前胶原羧基端原肽(PICP)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| CPA570Hu21 | Ⅰ型前胶原羧基端原肽(PICP)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| PAA570Hu01 | Ⅰ型前胶原羧基端原肽(PICP)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA570Hu08 | Ⅰ型前胶原羧基端原肽(PICP)多克隆抗体 | WB; IHC; ICC; IP. |
| SEA570Hu | Ⅰ型前胶原羧基端原肽(PICP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA570Hu | Ⅰ型前胶原羧基端原肽(PICP)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA570Hu | Ⅰ型前胶原羧基端原肽(PICP)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Nutrition | Dyslipidemic high-fat diet affects adversely bone metabolism in mice associated with impaired antioxidant capacity [ScienceDirect: S0899900709004717] |
| American Heart Journal | Serum markers of deranged myocardial collagen turnover: their relation to malignant ventricular arrhythmias in cardioverter-defibrillator recipients with heart failure. [PubMed: 23067911] |
| Journal of Hypertension | Circulating biomarkers of collagen metabolism in arterial hypertension: relevance of target organ damage [Pubmed: 23615327] |
| Pancreatology | Cdkn1a is a key mediator of rat pancreatic stellate cell senescence [Pubmed: 23719597] |
| The American Journal of Cardiology | Relation of burden of myocardial fibrosis to malignant ventricular arrhythmias and outcomes in Fabry disease [Pubmed:25073565] |
| PLoS One. | An NMR Metabolomic Study on the Effect of Alendronate in Ovariectomized Mice [Pubmed:25184758] |
| Journal of Bone and Mineral Research | The Anti-Osteoanabolic Function of Sclerostin is Blunted in Mice Carrying a High Bone Mass Mutation of Lrp5 [Pubmed:25640331] |
| Bone | The active role of osteoporosis in the interaction between osteoblasts and bone metastases [PubMed: 26057367] |
| J Nutr Biochem | Consumption of vitamin D2 [PubMed: 25792284] |
| Bibliothekssystem Universität Hamburg | Bilaterale Kommunikation zwischen Osteoblasten und Osteoklasten [volltexte:2016] |
| Inflammation Research | Fibrosis of extracellular matrix is related to the duration of the disease but is unrelated to the dynamics of collagen metabolism in dilated cardiomyopathy [pubmed:27516211] |
| Heart & Vessels | Left ventricular reverse remodeling is not related to biopsy-detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR [pubmed:28004175] |
| Scandinavian Journal of Rheumatology | The association between insulin-like growth factor 1 (IGF-1), IGF-binding proteins (IGFBPs), and the carboxyterminal propeptide of type I procollagen (PICP) in pre- and postmenopausal women with rheumatoid arthritis. [pubmed:27775453] |
| Allergy and Asthma Proceedings | Effect of montelukast on markers of airway remodeling in children withasthma. [pubmed:27657514] |
| Scandinavian Journal of Rheumatology | The association between insulin-like growth factor 1 (IGF-1), IGF-binding proteins (IGFBPs), and the carboxyterminal propeptide of type I procollagen (PICP) in pre- and postmenopausal women with rheumatoid arthritis [loi:irhe20] |
| Conditional Mouse Models Support the Role of SLC39A14 (ZIP14) in Hyperostosis Cranialis Interna and in Bone Homeostasis [ISBN: 978-94-6299-582-6] | |
| MOLECULAR MEDICINE REPORTS | Endothelial‑to‑mesenchymal transition in human idiopathic dilated cardiomyopathy [pubmed:29115553] |
| Pediatrics and Neonatology | Age-related changes in biochemical bone profile in thalassemic children [pubmed:28967496] |
| Heart and Vessels | Left ventricular reverse remodeling is not related to biopsy‑detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR [10.1007/s00380-016-0930-y] |
| Journal of Bone and Mineral Research | The Lrp4 R1170Q homozygous knock‐in mouse recapitulates the bone phenotype of sclerosteosis in humans [pubmed:28477420] |
| Cytokine | 12-month patterns of serum markers of collagen synthesis, transforming growth factor and connective tissue growth factor are similar in new-onset and chronic dilated cardiomyopathy in patients both with and without cardiac fibrosis. [pubmed:28460256] |
| Advanced Healthcare Materials | Prognostic value of fibrosis-related markers in dilated cardiomyopathy: A link between osteopontin and cardiovascular events. [pubmed:29120858] |
| Journal of Bone and Mineral Metabolism | Mutations That Alter the Carboxy‐Terminal‐Propeptide Cleavage Site of the Chains of Type I Procollagen Are Associated With a Unique Osteogenesis Imperfecta … [Pubmed:29669177] |
| Cardiovascular Journal of Africa | Assessment of myocardial fibrosis by late gadolinium enhancement imaging and biomarkers of collagen metabolism in chronic rheumatic mitral regurgitation. [Pubmed:29443354] |
| Journal of Cellular Physiology | DNA methylation regulates α‐smooth muscle actin expression during cardiac fibroblast differentiation [Pubmed: 30362530] |
| International Journal of Chronic Obstructive Pulmonary Disease | Altered serum levels of type I collagen turnover indicators accompanied by IL-6 and IL-8 release in stable COPD [Pubmed: 30655663] |
| Scientific Reports | Low fibrosis biomarker levels predict cardiac resynchronization therapy response [Pubmed: 30988339] |
| Bone | Agonist-induced activation of the S1P receptor 2 constitutes a novel osteoanabolic therapy for the treatment of osteoporosis in mice [Pubmed: 31028959] |
| PLoS One | Deficiency of sphingosine-1-phosphate receptor 3 does not affect the skeletal phenotype of mice lacking sphingosine-1-phosphate lyase [Pubmed: 31314788] |
| Bone | Mice lacking plastin-3 display a specific defect of cortical bone acquisition [Pubmed: 31678489] |
| Journal of Diabetes Research | Type 2 Diabetes Mellitus and Chronic Heart Failure with Midrange and Preserved Ejection Fraction: A Focus on Serum Biomarkers of Fibrosis [Pubmed: 33224989] |
| JOURNAL OF BONE AND MINERAL RESEARCH | Piezo1 Inactivation in Chondrocytes Impairs Trabecular Bone Formation [Pubmed: 33180356] |
| Microbiome changes in patients with chronic heart failure with preserved ejection fraction correlate with fibrosis markers: Description of a Russian cohort [] | |
| Ассоциации толщины эпикардиального жира и циркулирующих маркеров фиброза миокарда у пациентов с инфарктом миокарда [] | |
| Russian Journal of Cardiology | Early diagnosis of myocardial fibrosis in patients with epicardial obesity [] |
| J Diabetes Res | Association between Markers of Fibrosis and Heart Failure Incidence in Patients with Type 2 Diabetes Mellitus [34778465] |
| Clinical, Cosmetic and Investigational Dermatology | Extra Cellular Matrix Deposition and Assembly in Dermis Spheroids [34321901] |
| Scandinavian Cardiovascular Journal | Dual integrin αvβ3 and αvβ5 blockade attenuates cardiac dysfunction by reducing fibrosis in a rat model of doxorubicin-induced cardiomyopathy [34296634] |
| Eur J Appl Physiol | Effects of 8-week increment aerobic exercise program on bone metabolism and body composition in young non-athletes [Pubmed:35141785] |
| J. Clin. Med | Simple Predictors for Cardiac Fibrosis in Patients with Type 2 Diabetes Mellitus: The Role of Circulating Biomarkers and Pulse Wave Velocity [Pubmed:35628969] |
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