防御素β2(DEFb2)重组蛋白
Recombinant Defensin Beta 2 (DEFb2)
B-DF2; DEFB2; BD2; DEF-B2; HBD2; DEFB102; HBD-2; SAP1; DEFB4A; DEFB4; Skin-Antimicrobial Peptide 1
- 编号RPA072Po01
- 物种Sus scrofa; Porcine (Pig,猪) 相同的名称,不同的物种。
- 来源原核表达
- 宿主E.coli
- 内毒素水平<1.0EU/µg(LAL法测定)
- 亚细胞定位分泌
- 预测分子量35.0kDa
- 实际分子量35kDa(差异分析请参阅说明书)
- 片段与标签Ile22~Arg69 with N-terminal His and GST Tag
- 缓冲液成份100mMNaHCO3, 500mMNaCl, pH8.3, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 80%
- 等电点8.9
-
应用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白,请参见活性蛋白。 - 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 984 ¥ 2460 ¥ 4920 ¥ 14760 ¥ 36900
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
序列
用法
Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
增值服务
相关产品
编号 | 适用物种:Sus scrofa; Porcine (Pig,猪) | 应用(仅供研究使用,不用于临床诊断!) |
RPA072Po01 | 防御素β2(DEFb2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA072Po01 | 防御素β2(DEFb2)多克隆抗体 | WB; IHC; ICC; IP. |
MAA072Po21 | 防御素β2(DEFb2)单克隆抗体 | WB; IHC; ICC; IP. |
SEA072Po | 防御素β2(DEFb2)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA072Po | 防御素β2(DEFb2)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
杂志 | 参考文献 |
Livestock Science | The effect of Lactobacillus on the expression of porcine β-defensin-2 in the digestive tract of piglets [ScienceDirect: S1871141311000126] |
Appl Environ Microbiol. | Modulation of Porcine β-Defensins 1 and 2 upon Individual and Combined Fusarium Toxin Exposure in a Swine Jejunal Epithelial Cell Line [Asm: Source] |
Plos Pathogens | HIF-1α Is Essential for Effective PMN Bacterial Killing, Antimicrobial Peptide Production and Apoptosis in?Pseudomonas aeruginosa?Keratitis [PubMed: PMC3715414] |
British Journal of Nutrition | Identification of sociodemographic and clinical factors associated with the levels of human β-defensin-1 and human β-defensin-2 in the human milk of Han Chinese [Pubmed: 24124699] |
University of Waikato | The Assessment of Vitamin D, Antimicrobial Peptides and Procalcitonin in Bronchiectasis [Waikato:Source] |
Vaccine | On birth single dose live attenuated OPV and BCG vaccination induces gut cathelicidin LL37 responses at 6 week of age: A natural experiment [Pubmed:25444792] |
International Journal of Dairy Technology | Concentration characteristics of bovine β-defensin 1 and 2 in fresh bovine milk and infant formula [Doi: 10.1111] |
Molecular Nutrition Food Research | Green tea polyphenol epigallocatechin-3-gallate improves epithelial barrier function by inducing the productionof antimicrobial peptide pBD-1 and pBD-2 in monolayers of porcine intestinal epithelial IPEC-J2 cells. [Pubmed:26991948] |
Respiratory Research | Vitamin D and allergic airway disease shape the murine lung microbiome in a sex-specific manner. [pubmed:27655266] |
Key Engineering Materials | The Level of Inflammatory Cytokines and Antimicrobial Peptides after Composite Material Implantation and Contamination with Bacterial Culture. [16629795] |
Scientific Reports | Measurements of AMPs in stratum corneum of atopic dermatitis and healthy skin–tape stripping technique [Pubmed:29374283] |
Frontiers in Microbiology | Swine-Derived Probiotic Lactobacillus plantarum Inhibits Growth and Adhesion of Enterotoxigenic Escherichia coli and Mediates Host Defense [Pubmed:29997590] |
Journal of Dermatological Science | Glyoxal-induced exacerbation of pruritus and dermatitis is associated with staphylococcus aureus colonization in the skin of a rat model of atopic dermatitis [Pubmed:29496360] |
International Immunopharmacology | Caprylic acid and nonanoic acid upregulate endogenous host defense peptides to enhance intestinal epithelial immunological barrier function via histone deacetylase … [Pubmed: 30342347] |
拉脱维亚科学院院刊 | Influence of Antibiotic-Impregnated Biomaterials on Inflammatory Cytokines [] |
Veterinary Research | Bovine fetal mesenchymal stem cells exert antiproliferative effect against mastitis causing pathogen Staphylococcus aureus [Pubmed: 30975214] |
Journal of Immunology Research | Damage-Associated Molecular Patterns and Myeloid-Derived Suppressor Cells in Bronchoalveolar Lavage Fluid in Chronic Obstructive Pulmonary Disease Patients [] |
Veterinary Research | Toll-like receptor 5-mediated IL-17C expression in intestinal epithelial cells enhances epithelial host defense against F4+ ETEC infection [Pubmed: 31221216] |
Animals | Fat Encapsulation Reduces Diarrhea in Piglets Partially by Repairing the Intestinal Barrier and Improving Fatty Acid Transport [33375218] |
Sci Rep | Human beta defensin levels and vaginal microbiome composition in post-menopausal women diagnosed with lichen sclerosus [34362937] |
Medicine | Regulatory T cells, damage-associated molecular patterns, and myeloid-derived suppressor cells in bronchoalveolar lavage fluid interlinked with chronic obstructive … [Pubmed:35687771] |
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