促肾上腺皮质激素(ACTH)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Adrenocorticotropic Hormone (ACTH)
Corticotropin; Adrenocorticotropin
- 编号KSA836Ra11
- 物种Rattus norvegicus (Rat,大鼠) 相同的名称,不同的物种。
- 试剂内容 包被抗体、生物素标记的竞争物、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma and other biological fluids.
- 可适用原理竞争抑制酶联免疫吸附试验法测抗原
- 可检测范围12.35-1000pg/mL
- 可适灵敏度5.25pg/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 6566 ¥ 10944 ¥ 19699 ¥ 38304 ¥ 65664
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对促肾上腺皮质激素(ACTH)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Rattus norvegicus (Rat,大鼠) | 应用(仅供研究使用,不用于临床诊断!) |
CEA836Ra | 促肾上腺皮质激素(ACTH)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CCA836Ra | 促肾上腺皮质激素(ACTH)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMA836Ra | 促肾上腺皮质激素(ACTH)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSA836Ra11 | 促肾上腺皮质激素(ACTH)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
Respir Res. | Intracerebroventricular injection of leukotriene B4 attenuates antigen-induced asthmatic response via BLT1 receptor stimulating HPA-axis in sensitized rats. [PubMed: 20403205] |
Toxicology Letters | Regulation of corticosterone secretion is modified by PFOS exposure at different levels of the hypothalamic–pituitary–adrenal axis in adult male rats [Pubmed:24440345] |
Mol Med Rep. | Ursolic acid suppresses growth and adrenocorticotrophic hormone secretion in AtT20 cells as a potential agent targeting adrenocorticotrophic hormone-producing pituitary adenoma. [Pubmed:24682498] |
Peptides | Differential stress response in rats subjected to chronic mild stress is accompanied by changes in CRH-family gene expression at the pituitary level [Pubmed:25236411] |
Journal of Animal and Feed Sciences | Interdependence of the peripheral metabolism of glucocorticoids and thyroid hormones under calorie deficit in rats at different ages [Researchgate:Source] |
International Journal of Immunopathology & Pharmacology | Sea buckthorn (Hippophae rhamnoides L.) oil protects against chronic stress-induced inhibitory function of natural killer cells in rats [PubMed: 26684638] |
Cell Tissue Res | CXCL10/CXCR3 signaling mediates inhibitory action by interferon-gamma on CRF-stimulated adrenocorticotropic hormone (ACTH) release [PubMed: 26572542] |
Neuroscience Letters | Electroacupuncture reCavia (Guinea pig )late hypothalamic–pituitary–adrenal axis and enhance hippocampal serotonin system in a rat model of depression [Pubmed:26773866] |
Neural Regeneration Research | Moxibustion upreCavia (Guinea pig )lates hippocampal progranulin expression. [Pubmed:27212922] |
Environmental Science and Pollution Research | Lycopene ameliorates atrazine-induced oxidative damage in adrenal cortex of male rats by activation of the Nrf2/HO-1 pathway [Pubmed:27102619] |
Sci Rep. | Hedgehog signaling activation induces stem cell proliferation and hormone release in the adult pituitary gland [Pubmed:27109116] |
Sci Rep. | High activity of the stress promoter contributes to susceptibility to stress in the tree shrew [Pubmed:27125313] |
brain research bulletin | Activation of ATP-sensitive potassium channel by iptakalim normalizes stress-induced HPA axis disorder and depressive behaviour by alleviating inflammation and oxidative stress in mouse hypothalamus. [pubmed:28161195] |
中国临床药理学与治疗学 | 肉蔻五味丸抗抑郁作用及机制研究 [9787] |
Neuroscience | Heat stress-induced neuroinflammation and aberration in monoamine levels in hypothalamus are associated with temperature dysregulation [pubmed:28663093] |
Toxicology | Mixture effects of azole fungicides on the adrenal gland in a broad dose range [pubmed:28454766] |
Biomedicine & Pharmacotherapy | Triptolide suppresses growth and hormone secretion in murine pituitary corticotroph tumor cells via NF-kappaB signaling pathway [pubmed:28892788] |
Hormones and Behavior | Acth-induced model of depression resistant to tricyclic antidepressants: Neuroendocrine and behavioral changes and influence of long-term magnesium … [Pubmed:30025718] |
Frontiers in Pharmacology | Vaccinium bracteatum Leaf Extract Reverses Chronic Restraint Stress-Induced Depression-Like Behavior in Mice: Regulation of Hypothalamic-Pituitary … [Pubmed:30038568] |
Molecular and Cellular Endocrinology | Metformin suppresses growth and adrenocorticotrophic hormone secretion in mouse pituitary corticotroph tumor AtT20 cells [10.1016:j.mce.2018.07.007] |
Frontiers in Behavioral Neuroscience | Chronic hippocampal abnormalities and blunted HPA axis in an animal model of repeated unpredictable stress [Pubmed:30079015] |
Placenta | Human placentophagy: Effects of dehydration and steaming on hormones, metals and bacteria in placental tissue [Pubmed:29941176] |
BMC Veterinary Research | Hypothalamus-pituitary-adrenal axis involves in anti-viral ability through regulation of immune response in piglets infected by highly pathogenic porcine … [Pubmed:29540178] |
European Journal of Applied Physiology | Comparison between men and women of volume regulating hormones and aquaporin-2 excretion following graded central hypovolemia [Pubmed: 30564880] |
Cyber Leninka | ТЭС-ТЕРАПИЯ КАК МЕТОД ПРЕДУПРЕЖДЕНИЯ ДЕЗАДАПТАЦИИ У САМЦОВ КРЫС С ВЫСОКОЙ СТРЕССОУСТОЙЧИВОСТЬЮ [] |
Serbian Journal of Experimental and Clinical Research | Assessment of the TDCS Influence on Stress-Induced Disorders in Rats with Low Stress Sustainability and Endurance [] |
Scientific Reports | Mice lacking Casp1, Ifngr and Nos2 genes exhibit altered depressive-and anxiety-like behaviour, and gut microbiome composition [Pubmed: 31015500] |
alcoholism-clinical and experimental research | Persistent changes in stress‐regulatory genes in pregnant woman or a child with prenatal alcohol exposure [Pubmed: 31329297] |
Hormones and Behavior | Hypothalamus-pituitary-adrenal axis imbalance and inflammation contribute to sex differences in separation-and restraint-induced depression [Pubmed: 32165183] |
Prog Neuropsychopharmacol Biol Psychiatry | Progress in Neuropsychopharmacology & Biological Psychiatry [Pubmed: 17134810] |
Evid Based Complement Alternat Med | So-Ochim-Tang-Gamibang, a Traditional Herbal Formula, Ameliorates Depression by Regulating Hyperactive Glucocorticoid Signaling In Vitro and In Vivo [Pubmed: 33224257] |
PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY | Repeated unpredictable stress and social isolation induce chronic HPA axis dysfunction and persistent abnormal fear memory [Pubmed: 32682873] |
Aging Cell | Altered glucocorticoid metabolism represents a feature of macroph‐aging [Pubmed: 32463582] |
neurochemical research | Anxiolytic and Anti‑depressive Like Effects of Translocator Protein (18 kDa) Ligand YL‑IPA08 in a Rat Model of Postpartum Depression [Pubmed: 32383026] |
Behavioral Ecology and Sociobiology | Exposure to eagle owl feces induces anti-predator behavior, physiology, and hypothalamic gene responses in a subterranean rodent, the plateau zokor (Eospalax … [] |
EUROPEAN CHILD & ADOLESCENT PSYCHIATRY | Increased serum brain-derived neurotrophic factor and adrenocorticotropic hormone levels are associated with obsessive compulsive disorder in medication?free?¡ [33389158] |
International Journal of?Nanomedicine | Water-Soluble Carbon Dots in Cigarette Mainstream Smoke: Their Properties and the Behavioural, Neuroendocrinological, and Neurotransmitter Changes They ¡ [33762821] |
J Am Heart Assoc | Post©\Cardiac Arrest Hydrocortisone Use Ameliorates Cardiac Mitochondrial Injury in a Male Rat Model of Ventricular Fibrillation Cardiac Arrest [33960200] |
Effects of Propofol versus Sevoflurane on Postoperative Pain and Neuroendocrine Stress Response in Oocyte Pickup Patients [33936350] |
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