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α-黑素细胞刺激素(aMSH)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Alpha-Melanocyte Stimulating Hormone (aMSH)

α-MSH; Intermedins; Alpha-Melanotropin, Alpha-Melanocortin; Alpha-Intermedin

  • α-黑素细胞刺激素(aMSH)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • α-黑素细胞刺激素(aMSH)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对α-黑素细胞刺激素(aMSH)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
CEA239Hu α-黑素细胞刺激素(aMSH)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA239Hu α-黑素细胞刺激素(aMSH)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA239Hu11 α-黑素细胞刺激素(aMSH)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
J Biochem. Generation of a human bone marrow-derived mesenchymal stem cell line expressing and secreting high levels of bioactive α-melanocyte-stimulating hormone. [Pubmed: 23341471]
Pigment Cell Melanoma Res Pharmacological induction of skin pigmentation unveils the neuroendocrine circuit regulated by light [PubMed: 26582755]
The Journal of Biological Chemistry Intermedin Restores Hyperhomocysteinemia-induced Macrophage Polarization and Improves Insulin Resistance in Mice [Pubmed:27080257]
Pigment Cell & Melanoma Research Pharmacological induction of skin pigmentation unveils the neuroendocrine circuit reCavia (Guinea pig )lated by light [Pubmed:26582755]
Nature Communications Endocrine disruptors induce perturbations in endoplasmic reticulum and mitochondria of human pluripotent stem cell derivatives. [pubmed:28794470]
Cell Stem Cell Super-Obese Patient-Derived iPSC Hypothalamic Neurons Exhibit Obesogenic Signatures and Hormone Responses [Pubmed:29681516]
European Neuropsychopharmacology Epigenetic alterations of the POMC promoter in tobacco dependence [Pubmed:29871818]
Neuropeptides Mechanisms of sustained long-term weight loss after RYGB: α-MSH is a key factor [Pubmed:29685637]
Neuropsychobiology Alcohol Withdrawal and Proopiomelanocortin Neuropeptides in an Animal Model of Alcohol Dependence [Pubmed: 31117084]
INTERNATIONAL IMMUNOPHARMACOLOGY Intermedin alleviates the inflammatory response and stabilizes the endothelial barrier in LPS-induced ARDS through the PI3K/Akt/eNOS signaling pathway [Pubmed: 32892076]
J Endocrinol Invest Serum alpha-melanocyte-stimulating hormone (a-MSH), brain-derived neurotrophic factor (BDNF), and agouti-related protein (AGRP) levels in children with Prader … [Pubmed:35098494]
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