α-生育酚(TCPa)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Alpha-Tocopherol (TCPa)
VE; Vitamin E; α-TCP; α-Tocopherol
- 编号KSA922Ge11
- 物种Pan-species (General,通用) 相同的名称,不同的物种。
- 试剂内容 包被抗体、生物素标记的竞争物、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma and other biological fluids
- 可适用原理竞争抑制酶联免疫吸附试验法测抗原
- 可检测范围1.23-100ug/mL
- 可适灵敏度0.46ug/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 8597 ¥ 14328 ¥ 25790 ¥ 50148 ¥ 85968
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特异性
试剂盒中的抗体对α-生育酚(TCPa)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Pan-species (General,通用) | 应用(仅供研究使用,不用于临床诊断!) |
CPA922Ge11 | α-生育酚(TCPa)牛血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
CPA922Ge21 | α-生育酚(TCPa)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
PAA922Ge01 | α-生育酚(TCPa)多克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
LAA922Ge71 | α-生育酚(TCPa)多克隆抗体(生物素标记) | WB; IHC; ICC. |
CEA922Ge | α-生育酚(TCPa)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CCA922Ge | α-生育酚(TCPa)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMA922Ge | α-生育酚(TCPa)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSA922Ge11 | α-生育酚(TCPa)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
International Journal of Advanced Research | Evaluation of enzymatic and non- enzymatic antioxidant status in seminal plasma of Iraqi Infertile Men [Com:Source] |
Journal of Physiology and Pharmacology | Effect of vitamin E on cerebral cortical oxidative stress and brain-derived neurotrophic factor gene expression induced by hypoxia and exercise in rats. [PubMed: 25903950] |
Mycotoxin Res | The potential effects of antioxidant feed additives in mitigating the adverse effects of cornnaturally contaminated with Fusarium mycotoxins on antioxidant systems in the intestinal mucosa, plasma, and liver in weaned pigs. [Pubmed:27021614] |
The Journal of Nutritional Biochemistry | Vitamin E and caloric restriction promote hepatic homeostasis through expression of connexin 26, N-cad, E-cad and cholesterol metabolism genes [pubmed:27816814] |
Plant Physiol Biochem | Ultrasonication of in vitro potato single node explants: Activation and recovery of antioxidant defence system and growth responses [pubmed:29102903] |
PLoS One | Oxidative stress in lung cancer patients is associated with altered serum markers of lipid metabolism [Pubmed: 30973911] |
Prooxidant effect of α-tocopherol in pancreatic tumors. [] | |
Vitamin A, C, D, E and B12 Levels in Leprosy: A Case Control Study [] |
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