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血管紧张素原(AGT)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Angiotensinogen (AGT)

ANHU; SERPINA8; Angiotensin; Renin Substrate; Serpin Peptidase Inhibitor,Clade A,Member 8(Alpha-1 Antiproteinase,Antitrypsin)

  • 血管紧张素原(AGT)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 血管紧张素原(AGT)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对血管紧张素原(AGT)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种：Rattus norvegicus (Rat，大鼠) 应用(仅供研究使用，不用于临床诊断！)
APA797Ra61 血管紧张素原(AGT)活性蛋白 Cell culture; Activity Assays.
RPA797Ra01 血管紧张素原(AGT)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPA797Ra61 血管紧张素原(AGT)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA797Ra01 血管紧张素原(AGT)多克隆抗体 WB; IHC; ICC; IP.
LAA797Ra71 血管紧张素原(AGT)多克隆抗体(生物素标记) WB; IHC; ICC.
LAA797Ra81 血管紧张素原(AGT)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
MAA797Ra21 血管紧张素原(AGT)单克隆抗体 WB; IHC; ICC; IP.
MAA797Ra22 血管紧张素原(AGT)单克隆抗体 WB; IHC; ICC; IP.
MAA797Ra23 血管紧张素原(AGT)单克隆抗体 WB; IHC; ICC; IP.
MAA797Ra24 血管紧张素原(AGT)单克隆抗体 WB; IHC; ICC; IP.
MAA797Ra27 血管紧张素原(AGT)单克隆抗体 WB; IHC; ICC; IP.
MAA797Ra26 血管紧张素原(AGT)单克隆抗体 WB; IHC; ICC; IP.
SEA797Ra 血管紧张素原(AGT)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA797Ra 血管紧张素原(AGT)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA797Ra01 血管紧张素原(AGT)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Transplant International Elevated urinary angiotensinogen a marker of intrarenal renin angiotensin system in hypertensive renal transplant recipients: does it play a role in development of proteinuria in hypertensive renal transplant patients? [Wiley: source]
Amyloid The relation between urinary angiotensinogen and proteinuria in renal AA amyloidosis patients [PubMed: 22320202]
Pediatric Mephrology Urinary angiotensinogen as a marker of intrarenal angiotensin II activity in adolescents with primary hypertension [PubMed: PMC3661928]
Acta Paediatrica Urinary angiotensinogen as a novel marker of obstructive nephropathy in children [Pubmed: 23772991]
Journal of the Renin-Angiotensin-Aldosterone System Association between urinary angiotensinogen, hypertension and proteinuria in pregnant women with preeclampsia [Sagepub: Source]
Pediatric Nephrology MP707 CHARACTERISTICS AND OUTCOMES OF CHILDREN WITH AUTOSOMAL RECESSIVE POLYCYSTIC KIDNEY DISEASE COHORT FROM THE ESPN/ERA-EDTA REGISTRY [Oxfordjournals:Source]
journal of human hypertension Relationship between blood pressure var [PubMed: 26223347]
PLoS One Higher Serum Angiotensinogen Is an Indicator of IgA Vasculitis with Nephritis Revealed by Comparative Proteomes Analysis [PubMed: 26098644]
MKA Korelasi pengukuran antropometri dengan tekanan darah dan angiotensinogen plasma pada dewasa [Index.Php: Art]
Journal of Human Hypertension Relationship between blood pressure variability and renal activity of the renin-angiotensin system. [pubmed:26223347]
Acta Pharmacol Sin. Pharmacological evidence: a new therapeutic approach to the treatment of chronic heart failure through SUR2B/Kir6.1 channel in endothelial cells. [pubmed:27890915]
Journal of Chromatography A Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858]
Life Sciences Pyrrolidine dithiocarbamate inhibits mouse acute kidney injury induced by diclofenac by targeting oxidative damage, cytokines and NF-κB activity [Pubmed:30036488]
American Journal of Physiology-Renal Physiology Role of (Pro) Renin Receptor in Albumin Overload-Induced Nephropathy in Rats [Pubmed:29846109]
Methods in Molecular Biology Quantitation of Total and Free Thiol Angiotensinogen as a Prognostic Marker for Preeclampsia [Pubmed: 31069778]
J Transl Med Multi-omic signatures of atherogenic dyslipidaemia: pre-clinical target identification and validation in humans [33407555]
Hypertens Res Association of Ang-(1¨C7) and des-Arg 9 BK as new biomarkers of obesity and cardiometabolic risk factors in adolescents [33568792]
Physiol Rep (Pro) renin receptor antagonist PRO20 attenuates nephrectomy©\induced nephropathy in rats via inhibition of intrarenal RAS and Wnt/¦Â©\catenin signaling [34057312]
Cancer Cell Int A novel strategy to identify candidate diagnostic and prognostic biomarkers for gastric cancer [34215253]
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