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载脂蛋白E(APOE)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Apolipoprotein E (APOE)

Apo-E; AD2; Apoprotein; Alzheimer Disease 2(E4-Associated,Late Onset

  • 载脂蛋白E(APOE)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 载脂蛋白E(APOE)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对载脂蛋白E(APOE)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
EPA704Hu61 载脂蛋白E(APOE)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
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RPA704Hu01 载脂蛋白E(APOE)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA704Hu01 载脂蛋白E(APOE)多克隆抗体 WB; IHC; ICC; IP.
MAA704Hu21 载脂蛋白E(APOE)单克隆抗体 WB; IHC; ICC; IP.
MAA704Hu22 载脂蛋白E(APOE)单克隆抗体 WB; IHC; ICC; IP.
SEA704Hu 载脂蛋白E(APOE)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA704Hu 载脂蛋白E(APOE)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA704Hu 载脂蛋白E(APOE)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA704Hu01 载脂蛋白E(APOE)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Seizure - European Journal of Epilepsy Cerebrospinal fluid apolipoprotein E concentration decreases after seizure [Seizure: PIIS1059131109002441]
Biochimica et Biophysica Acta Hepatic lipase- and endothelial lipase-deficiency in mice promotes macrophage-to-feces RCT and HDL antioxidant properties [PubMed: 23328279 ]
Medical Oncology Expression and clinical significance of apolipoprotein E in pancreatic ductal adenocarcinoma [Pubmed: 23609192]
Diabetes. Adipocyte Atp-Binding Cassette G1 Promotes Triglyceride Storage, Fat Mass Growth And Human Obesity [Pubmed:25249572]
renal failure Frequencies of apolipoprotein E alleles in depressed patients undergoing hemodialysis – a case-control study [PubMed: 25707516]
Biochim Biophys Acta Culturing of HepG2 cells with human serum improve their functionality and suitability in studies of lipid metabolism [PubMed: 26515253]
Diabetes Adipocyte ATP-binding cassette G1 promotes triglyceride storage, fat mass growth, and human obesity [PubMed: 25249572]
Scientific Reports Adaptive immunity against gut microbiota enhances apoE-mediated immune regulation andreduces atherosclerosis and western-diet-related inflammation. [pubmed:27383250]
Biochemical Pharmacology  Differential effects of apoE and apoJ mimetic peptides on the action of an anti-Aβ scFv in 3xTg-AD mice [Pubmed:30026023]
Oncotarget Deletion of plasma Phospholipid Transfer Protein (PLTP) increases microglial phagocytosis and reduces cerebral amyloid-β deposition in the J20 mouse … [Pubmed:29731975]
Proteomics Clinical Applications Comparative proteomics analysis of serum proteins in gestational diabetes during early and middle stages of pregnancy [Pubmed: 31162828]
Nature Communications Hepatic HuR modulates lipid homeostasis in response to high-fat diet [Pubmed: 32546794]
VETERINARY PATHOLOGY Pathology and Proteomics-Based Diagnosis of Localized Light-Chain Amyloidosis in Dogs and Cats [Pubmed: 32880234]
Journal of Clinical Medicine The Significance of Apolipoprotein E Measurement in the Screening of Fetal Down Syndrome [33321701]
Cancer Cell Int Discovery and validation of FBLN1 and ANT3 as potential biomarkers for early detection of cervical cancer [33602229]
Nat Commun GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity [34853313]
Talanta Iridium nanoclusters as high sensitive-tunable elemental labels for immunoassays: Determination of IgE and APOE in aqueous humor by inductively coupled … [Pubmed:35378356]
Analytica Chimica Acta Gold nanoclusters as elemental label for the sequential quantification of apolipoprotein E and metallothionein 2A in individual human cells of the retinal … [Pubmed:35361429]
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