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β-内啡肽(bEP)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Beta-Endorphin (bEP)

b-EP

  • β-内啡肽(bEP)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • β-内啡肽(bEP)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对β-内啡肽(bEP)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
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KSA806Hu11 β-内啡肽(bEP)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
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International Journal of Pharmaceutical Science Invention ISSN(Online) Effect of Ramadan fasting on endorphin and endocannabinoid level in serum, PBMC and macrophage [Ijpsi: Source]
Neurological Sciences Evaluation of immune parameters in chronic migraine with medication overuse [Pubmed:24867859]
Brain Behav. Cerebrospinal fluid and plasma b-endorphin levels in children with cerebral malaria. [pubmed:28413714]
Journal of Cosmet Dermatol. Ultraviolet A photosensitivity profile of dexchlorpheniramine maleate and promethazine-based creams: Anti-inflammatory,antihistaminic, and skin barrier protection properties [DOI: 10.1111/jocd.12349]
International Journal of Nursing Practice Non‐pharmacological interventions during childbirth for pain relief, anxiety, and neuroendocrine stress parameters: A randomized controlled trial [Pubmed:29512230]
КОРРЕКЦИЯ ЦИТОКИНОВОГО И ГОРМОНАЛЬНОГО ДИСБАЛАНСА ПРИ ЛЕЧЕНИИ СТАБИЛЬНОЙ СТЕНОКАРДИИ НАПРЯЖЕНИЯ [10.25207:1608-6228-2018-25-3-51-55]
Journal of Pediatric Neurosciences Plasma and cerebrospinal fluid beta-endorphin levels show a strong association in children with cerebral malaria []
Chinese Journal of Physiology Blood dopamine level enhanced by caffeine in men after treadmill running [Pubmed: 31793465]
Free Radical Biology and Medicine Blue light (λ= 453 nm) nitric oxide dependently induces β-endorphin production of human skin keratinocytes in-vitro and increases systemic β-endorphin levels in … [Pubmed: 31553937]
 Journal of Sexual Medicine Between pleasure and pain: a pilot study on the biological mechanisms associated with BDSM interactions in dominants and submissives [Pubmed: 32044259]
JOURNAL OF AFFECTIVE DISORDERS Pain Sensitivity and Plasma Beta-Endorphin in Adolescent Non-Suicidal Self-Injury [Pubmed: 32961416]
EUROPEAN NEUROPSYCHOPHARMACOLOGY Serum β-endorphin levels are associated with addiction to suicidal behavior: a pilot study [Pubmed: 32855024]
J Burn Care Res Efficacy of Acupuncture on Pain Mechanisms, Inflammatory Responses, and Wound Healing in the Acute Phase of Major Burns: An Experimental Study on Rats [34309681]
European Journal of Integrative Medicine Effects of transcutaneous electrical nerve stimulation applied at different frequencies during labor on hormone levels, labor pain perception, and anxiety: a randomized … []
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