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β-内啡肽(bEP)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Beta-Endorphin (bEP)

b-EP

  • 编号KSA806Ra11
  • 物种Rattus norvegicus (Rat，大鼠) 相同的名称，不同的物种。
  • 试剂内容 包被抗体、生物素标记的竞争物、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
  • 可检测样本Serum, plasma, tissue homogenates, cell lysates, cerebrospinal fluid, cell culture supernates and other biological fluids
  • 可适用原理竞争抑制酶联免疫吸附试验法测抗原
  • 可检测范围12.35-1000pg/mL
  • 可适灵敏度4.62pg/mL
  • 应用Main materials for "Do It (ELISA Kit) Yourself".
  • 下载 英文说明书   中文说明书
  • 规格 96T*596T*10 96T*20 96T*50 96T*100
  • 价格 ¥ 7387 ¥ 12312 ¥ 22162 ¥ 43092 ¥ 73872
  • 欲了解实际交易价格和更多情况，请与当地经销商联系!
  • β-内啡肽(bEP)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • β-内啡肽(bEP)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对β-内啡肽(bEP)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种：Rattus norvegicus (Rat，大鼠) 应用(仅供研究使用，不用于临床诊断！)
CPA806Ra21 β-内啡肽(bEP)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA806Ra01 β-内啡肽(bEP)多克隆抗体 IHC; ICC; IP.
CEA806Ra β-内啡肽(bEP)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA806Ra β-内啡肽(bEP)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA806Ra β-内啡肽(bEP)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA806Ra11 β-内啡肽(bEP)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Experimental Dermatology Endogenous μ-opioid peptides modulate immune response towards malignant melanoma [Publish: RD10279]
European Journal of Physiology Physiology and cell biology of acupuncture observed in calcium signaling activated by acoustic shear wave [SpringerLink: tv015l3126833315]
International Journal of Pharmaceutical Science Invention ISSN(Online) Effect of Ramadan fasting on endorphin and endocannabinoid level in serum, PBMC and macrophage [Ijpsi: Source]
Neurological Sciences Evaluation of immune parameters in chronic migraine with medication overuse [Pubmed:24867859]
Brain Behav. Cerebrospinal fluid and plasma b-endorphin levels in children with cerebral malaria. [pubmed:28413714]
Journal of Cosmet Dermatol. Ultraviolet A photosensitivity profile of dexchlorpheniramine maleate and promethazine-based creams: Anti-inflammatory,antihistaminic, and skin barrier protection properties [DOI: 10.1111/jocd.12349]
International Journal of Nursing Practice Non‐pharmacological interventions during childbirth for pain relief, anxiety, and neuroendocrine stress parameters: A randomized controlled trial [Pubmed:29512230]
КОРРЕКЦИЯ ЦИТОКИНОВОГО И ГОРМОНАЛЬНОГО ДИСБАЛАНСА ПРИ ЛЕЧЕНИИ СТАБИЛЬНОЙ СТЕНОКАРДИИ НАПРЯЖЕНИЯ [10.25207:1608-6228-2018-25-3-51-55]
Journal of Pediatric Neurosciences Plasma and cerebrospinal fluid beta-endorphin levels show a strong association in children with cerebral malaria []
Chinese Journal of Physiology Blood dopamine level enhanced by caffeine in men after treadmill running [Pubmed: 31793465]
Free Radical Biology and Medicine Blue light (λ= 453 nm) nitric oxide dependently induces β-endorphin production of human skin keratinocytes in-vitro and increases systemic β-endorphin levels in … [Pubmed: 31553937]
 Journal of Sexual Medicine Between pleasure and pain: a pilot study on the biological mechanisms associated with BDSM interactions in dominants and submissives [Pubmed: 32044259]
JOURNAL OF AFFECTIVE DISORDERS Pain Sensitivity and Plasma Beta-Endorphin in Adolescent Non-Suicidal Self-Injury [Pubmed: 32961416]
EUROPEAN NEUROPSYCHOPHARMACOLOGY Serum β-endorphin levels are associated with addiction to suicidal behavior: a pilot study [Pubmed: 32855024]
J Burn Care Res Efficacy of Acupuncture on Pain Mechanisms, Inflammatory Responses, and Wound Healing in the Acute Phase of Major Burns: An Experimental Study on Rats [34309681]
European Journal of Integrative Medicine Effects of transcutaneous electrical nerve stimulation applied at different frequencies during labor on hormone levels, labor pain perception, and anxiety: a randomized … []
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