β-羟丁酸(bHB)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Beta-Hydroxybutyric Acid (bHB)
OHB; BHBA; Beta-Hydroxybutyrate; 3-Hydroxybutyric Acid; β-Hydroxybutyric acid
- 编号KSB022Ge11
- 物种Pan-species (General,通用) 相同的名称,不同的物种。
- 试剂内容 包被抗体、生物素标记的竞争物、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可适用原理竞争抑制酶联免疫吸附试验法测抗原
- 可检测范围1.23-100ug/mL
- 可适灵敏度0.48ug/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 8726 ¥ 14544 ¥ 26179 ¥ 50904 ¥ 87264
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特异性
试剂盒中的抗体对β-羟丁酸(bHB)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Pan-species (General,通用) | 应用(仅供研究使用,不用于临床诊断!) |
CPB022Ge11 | β-羟丁酸(bHB)牛血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
CPB022Ge21 | β-羟丁酸(bHB)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
PAB022Ge01 | β-羟丁酸(bHB)多克隆抗体 | WB; IHC; ICC; IP. |
CEB022Ge | β-羟丁酸(bHB)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB022Ge | β-羟丁酸(bHB)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSB022Ge11 | β-羟丁酸(bHB)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
Endocrinology | Orphan nuclear receptor Nur77 mediates fasting-induced hepatic fibroblast growth factor 21 expression [Endocrine:Source] |
Chem Commun (Camb) | A bioelectronic system for insulin release triggered by ketone body mimicking diabetic ketoacidosis in vitro [PubMed: 25846235] |
PLoS One | Regulation of Nutritional Metabolism in Transition Dairy Cows: Energy Homeostasis and Health in Response to Post-Ruminal Choline and Methionine [pubmed:27501393] |
Buffalo Bulletin | Alterations in haemato-biochemical profile following by-pass nutrients supplementation in early lactating Murrah buffaloes [] |
Journal of Integrative Agriculture | Temporal dynamics of nutrient balance, plasma biochemical and immune traits, and liver function in transition dairy cows [] |
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