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骨成型蛋白2(BMP2)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Bone Morphogenetic Protein 2 (BMP2)

BMP2A; BMP-2A; Hemochromatosis Modifier

  • 骨成型蛋白2(BMP2)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 骨成型蛋白2(BMP2)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对骨成型蛋白2(BMP2)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
APA013Hu01 骨成型蛋白2(BMP2)活性蛋白 Cell culture; Activity Assays.
RPA013Hu02 骨成型蛋白2(BMP2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA013Hu01 骨成型蛋白2(BMP2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA013Hu03 骨成型蛋白2(BMP2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA013Hu02 骨成型蛋白2(BMP2)活性蛋白 Cell culture; Activity Assays.
PAA013Hu02 骨成型蛋白2(BMP2)多克隆抗体 WB; IHC; ICC; IP.
PAA013Hu01 骨成型蛋白2(BMP2)多克隆抗体 WB; IHC; ICC; IP.
MAA013Hu22 骨成型蛋白2(BMP2)单克隆抗体 WB; IHC; ICC; IP.
SEA013Hu 骨成型蛋白2(BMP2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA013Hu 骨成型蛋白2(BMP2)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA013Hu 骨成型蛋白2(BMP2)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA013Hu01 骨成型蛋白2(BMP2)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

参考文献

杂志 参考文献
Journal of Applied Toxicology Chronic exposure to low concentrations of strontium 90 affects bone physiology but not the hematopoietic system in mice [Wiley: Source]
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source]
Medical Journal of Chinese People's Liberation Army Effect of transplantation of BMP-2-induced bone marrow mesenchymal stem cells on myocardial infarction of rats after reperfusion [Source]
PLOS ONE PDGF-AA promotes osteogenic differentiation and migration of mesenchymal stem cell by down-regulating PDGFRα and derepressing BMP-Smad1/5/8 signaling [Pubmed:25470749]
Eur J Pharmacol Negative effect of serotonin–norepinephrine reuptake inhibitor therapy on rat bone tissue after orchidectomy [PubMed: 25934570]
Pharmacology Effect of Mirtazapine on Rat Bone Tissue after Orchidectomy [PubMed: 25871861]
Journal of Cellular Biochemistry Titanium with Nanotopography Induces Osteoblast Differentiation by Regulating Endogenous Bone Morphogenetic Protein Expression and Signaling Pathway [PubMed: 26681207]
International Journal of Nanomedicine Sustained dual release of placental growth factor-2 and bone morphogenic protein-2 from heparin-based nanocomplexes for direct osteogenesis [Pubmed:27042064]
Research report EFFECTS OF FLUORIDE ON THE EXPRESSION OF BMP-2 AND SMAD1 IN RAT OSTEOBLASTS IN VITRO [files:p013-022]
Gene  Unveiling the interactions among BMPR-2, ALK-1 and 5-HTT genes in the pathophysiology of HAPE [Pubmed:27196063]
Journal of Materials Chemistry B Extracellular Matrix Powder from Cultured Cartilage-Like Tissue as Cell Carriers for Cartilage Repair [DOI:10.1039/C7TB00640C]
Scientific Reports 3D- Printed Poly(ε-caprolactone) Scaffold Integrated with Cell-laden Chitosan Hydrogels for Bone Tissue Engineering. [pubmed:29042614]
Journal of Applied Biomaterials & Functional Materials Demineralized dentin and enamel matrices as suitable substrates for bone regeneration [pubmed:28731486]
Journal of Clinical Periodontology  Peroxisome proliferator‐activated receptor γ plays dual roles on experimental periodontitis in rats [Pubmed:29574908]
Pharmacology Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive Rats [Pubmed:29898457]
Journal of Applied Biomaterials & Functional Materials BMP-2 and type I collagen preservation in human deciduous teeth after demineralization [Pubmed:30045659]
Applied Surface Science Enhanced osteogenic differentiation of MC3T3-E1 on rhBMP-2 immobilized titanium surface through polymer-mediated electrostatic interaction []
Materials Science and Engineering: C Injectable hydrogels from enzyme-catalyzed crosslinking as BMSCs-laden scaffold for bone repair and regeneration []
Biomaterials Science Development of CaCO 3 microsphere-based composite hydrogel for dual delivery of growth factor and Ca to enhance bone regeneration []
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY The dose-time effects of fluoride on the expression and DNA methylation level of the promoter region of BMP-2 and BMP-7 in rats [Pubmed: 32004919]
Journal of Industrial and Engineering Chemistry Biofabrication and application of decellularized bone extracellular matrix for effective bone regeneration []
BMC Musculoskeletal Disorders Synergistic Effect of Platelet-rich Fibrin Releasate and Bone Marrow Stem Cells in Preventing Bone Loss in Ovariectomized Mouse []
Bioact Mater New design to remove leukocytes from platelet-rich plasma (PRP) based on cell dimension rather than density [33842739]
Tissue Eng Regen Med Osteogenic and Angiogenic Potency of VEGF165-Transfected Canine Bone Marrow Mesenchymal Cells Combined with Coral Hydroxyapatite in Vitro [34302695]
Toxicon The WNT/β-catenin signalling pathway induces chondrocyte apoptosis in the cartilage injury caused by T-2 toxin in rats [34742779]
ACS Biomater Sci Eng Polydopamine-Coated Poly(l-lactide) Nanofibers with Controlled Release of VEGF and BMP-2 as a Regenerative Periosteum [34472855]
Phytomedicine Efficacy of Total flavonoids of Rhizoma drynariae on the Blood Vessels and the Growth Quality of Bone Graft in the Induced Membrane [Pubmed:35278899]
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