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连环蛋白β1(β-catenin)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Beta Catenin (β-catenin)

CTNNb1; bCat; b-Cat; CTNN-B1; Catenin Beta 1; Cadherin-Associated Protein

  • 连环蛋白β1(β-catenin)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 连环蛋白β1(β-catenin)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对连环蛋白β1(CTNNb1)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种：Mus musculus (Mouse，小鼠) 应用(仅供研究使用，不用于临床诊断！)
RPB021Mu02 连环蛋白β1(β-catenin)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB021Mu01 连环蛋白β1(β-catenin)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB021Mu02 连环蛋白β1(β-catenin)多克隆抗体 WB; IHC; ICC; IP.
PAB021Mu01 连环蛋白β1(β-catenin)多克隆抗体 WB; IHC; ICC; IP.
SEB021Mu 连环蛋白β1(β-catenin)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB021Mu 连环蛋白β1(β-catenin)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSB021Mu01 连环蛋白β1(β-catenin)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

参考文献

杂志 参考文献
Clinica Chimica Acta Versican and its associated molecules: Potential diagnostic markers for multiple myeloma [Pubmed:25623955]
Journal of Bone and Mineral Research Adverse effects of osteocytic constitutive activation of ?-catenin on bone strength and bone growth [Pubmed:25639729]
Am J Transl Res Role of SDF-1 and Wnt signaling pathway in the myocardial fibrosis of hypertensive rats [PubMed: 26396666]
Scientific Reports Is there an association between enhanced choline and β-catenin pathway in breast cancer? A pilot study by MR Spectroscopy and ELISA. [pubmed:28533512]
Life Sciences Hepatoprotective effect of hesperidin in hepatocellular carcinoma: Involvement of Wnt signaling pathways. [pubmed:28754618]
Scientific Reports Is there an association between enhanced choline and β-catenin pathway in breast cancer? A pilot study by MR Spectroscopy and ELISA [Pubmed: 28533512]
The Showa University Journal of Medical Sciences Eribulin Treatment Induces High Expression of miR-195 and Inactivates the Wnt/β-catenin Signaling Pathway in Triple-negative Breast Cancer [Doi: 10.15369/sujms.30.359]
Advances in Medical Sciences Urinary procollagen III aminoterminal propeptide and β-catenin–New diagnostic biomarkers in solitary functioning kidney? [Pubmed: 30738287]
Biomed Pharmacother Silence of FAM83H-AS1 promotes chemosensitivity of gastric cancer through Wnt/β-catenin signaling pathway [Pubmed: 32028241]
Journal of Cosmetic Dermatology Novel complex of cosmetic ingredients with promising action in preventing hair loss and follicular aging through mechanism involving enrichment of WNT/signaling … [Pubmed: 33179848]
Nutrients Hydroxytyrosol as a Promising Ally in the Treatment of Fibromyalgia [Pubmed: 32784915]
Oncotarget Amalgamation of PI3K and EZH2 blockade synergistically regulates invasion and angiogenesis: combination therapy for glioblastoma multiforme [33473259]
Cytokine QNZ alleviated hepatocellular carcinoma by targeting inflammatory pathways in a rat model [34564023]
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