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促肾上皮质激素释放激素(CRF)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Corticotropin Releasing Factor (CRF)

CRH; Corticotropin Releasing Hormone; Corticoliberin

  • 促肾上皮质激素释放激素(CRF)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 促肾上皮质激素释放激素(CRF)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对促肾上皮质激素释放激素(CRH)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种：Mus musculus (Mouse，小鼠) 应用(仅供研究使用，不用于临床诊断！)
RPA835Mu01 促肾上皮质激素释放激素(CRF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA835Mu02 促肾上皮质激素释放激素(CRF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
CPA835Mu21 促肾上皮质激素释放激素(CRF)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
CPA835Mu22 促肾上皮质激素释放激素(CRF)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
CPA835Mu33 促肾上皮质激素释放激素(CRF)钥孔血蓝蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA835Mu09 促肾上皮质激素释放激素(CRF)多克隆抗体 WB; IHC; ICC; IP.
PAA835Mu01 促肾上皮质激素释放激素(CRF)多克隆抗体 WB; IHC; ICC; IP.
PAA835Mu02 促肾上皮质激素释放激素(CRF)多克隆抗体 WB; IHC; ICC; IP.
PAA835Mu07 促肾上皮质激素释放激素(CRF)多克隆抗体 WB; IHC; ICC; IP.
PAA835Mu08 促肾上皮质激素释放激素(CRF)多克隆抗体 WB; IHC; ICC; IP.
LAA835Mu81 促肾上皮质激素释放激素(CRF)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA835Mu71 促肾上皮质激素释放激素(CRF)多克隆抗体(生物素标记) WB; IHC; ICC.
MEA835Mu 促肾上皮质激素释放激素(CRF)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA835Mu 促肾上皮质激素释放激素(CRF)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA835Mu 促肾上皮质激素释放激素(CRF)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA835Mu11 促肾上皮质激素释放激素(CRF)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Toxicology Letters Regulation of corticosterone secretion is modified by PFOS exposure at different levels of the hypothalamic–pituitary–adrenal axis in adult male rats [Pubmed:24440345]
Bipolar?Disord. Immune dysfunction in bipolar disorder and suicide risk: is there an association between peripheral corticotropin‐releasing hormone and interleukin‐1β? [Pubmed:24862833]
Peptides Differential stress response in rats subjected to chronic mild stress is accompanied by changes in CRH-family gene expression at the pituitary level [Pubmed:25236411]
Ann Dermatol Acute Stress-Induced Changes in Follicular Dermal Papilla Cells and Mobilization of Mast Cells:Implications for Hair Growth. [pubmed:27746640]
Neuroscience Heat stress-induced neuroinflammation and aberration in monoamine levels in hypothalamus are associated with temperature dysregulation [pubmed:28663093]
Biomed Research International Colonic Transit Time and Gut Peptides in Adult Patients with Slow and Normal Colonic Transit Constipation [pubmed:29082242]
Biochemical Pharmacology Effects of n-3 PUFA enriched and n-3 PUFA deficient diets in naïve and Aβ-treated female rats [Pubmed:30028991]
Frontiers in Pharmacology Vaccinium bracteatum Leaf Extract Reverses Chronic Restraint Stress-Induced Depression-Like Behavior in Mice: Regulation of Hypothalamic-Pituitary … [Pubmed:30038568]
Toxicology Letters Perfluorooctane sulfonate (PFOS) can alter the hypothalamic-pituitary-adrenal (HPA) axis activity by modifying CRF1 and glucocorticoid receptors [Pubmed:29807116]
Neuropsychiatric disease and treatment The depressive-like behaviors of chronic unpredictable mild stress-treated mice, ameliorated by Tibetan medicine Zuotai: involvement in the hypothalamic … [Pubmed:29379286]
BMC Veterinary Research Hypothalamus-pituitary-adrenal axis involves in anti-viral ability through regulation of immune response in piglets infected by highly pathogenic porcine … [Pubmed:29540178]
Bioscience Research Bioscience Research []
FASEB Journal Contribution of DNA methylation in chronic stress–induced cardiac remodeling and arrhythmias in mice [Pubmed: 31431066]
Aging Cell Altered glucocorticoid metabolism represents a feature of macroph‐aging [Pubmed: 32463582]
Endocr J Impairment of CRH in the intestinal mucosal epithelial barrier of pregnant Bama miniature pig induced by restraint stress [33408312]
CRYOBIOLOGY O-GlcNAc/Akt pathway regulates glucose metabolism and reduces apoptosis in liver of piglets with acute cold stress [33651993]
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