半胱氨酸蛋白酶抑制剂B(CSTB)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Cystatin B (CSTB)
Cys-B; CST6; EPM1; PME; STFB; CPI-B; Stefin B; Liver thiol proteinase inhibitor
- 编号KSJ322Ra01
- 物种Rattus norvegicus (Rat,大鼠) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.312-20ng/mL
- 可适灵敏度0.119ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 6149 ¥ 10248 ¥ 18446 ¥ 35868 ¥ 61488
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对半胱氨酸蛋白酶抑制剂B(CSTB)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Rattus norvegicus (Rat,大鼠) | 应用(仅供研究使用,不用于临床诊断!) |
RPJ322Ra01 | 半胱氨酸蛋白酶抑制剂B(CSTB)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAJ322Ra01 | 半胱氨酸蛋白酶抑制剂B(CSTB)多克隆抗体 | WB; IHC; ICC; IP. |
LAJ322Ra71 | 半胱氨酸蛋白酶抑制剂B(CSTB)多克隆抗体(生物素标记) | WB; IHC; ICC. |
MAJ322Ra24 | 半胱氨酸蛋白酶抑制剂B(CSTB)单克隆抗体 | WB |
MAJ322Ra26 | 半胱氨酸蛋白酶抑制剂B(CSTB)单克隆抗体 | WB; IHC; ICC; IP. |
MAJ322Ra21 | 半胱氨酸蛋白酶抑制剂B(CSTB)单克隆抗体 | WB; IHC; ICC; IP. |
LAJ322Ra74 | 半胱氨酸蛋白酶抑制剂B(CSTB)单克隆抗体(生物素标记) | WB; IHC; ICC. |
SEJ322Ra | 半胱氨酸蛋白酶抑制剂B(CSTB)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMJ322Ra | 半胱氨酸蛋白酶抑制剂B(CSTB)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
PSJ322Ra01 | 半胱氨酸蛋白酶抑制剂B(CSTB)抗体对 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSJ322Ra01 | 半胱氨酸蛋白酶抑制剂B(CSTB)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
AIDS. | Cathepsin B and cystatin B in HIV-seropositive women are associated with infection and HIV-1-associated neurocognitive disorders [PubMed: 23291538] |
International Journal of Circumpolar Health | Evaluation of serum procathepsin B, cystatin B and cystatin C as possible biomarkers of ovarian cancer [PubMed: PMC3754495] |
PLoS One. | Dysregulation of Macrophage-Secreted Cathepsin B Contributes to HIV-1-Linked Neuronal Apoptosis [PubMed: PMC3365072] |
Journal of Neuroimmune Pharmacology | Cocaine potentiates cathepsin B secretion and neuronal apoptosis from HIV-infected macrophages [Pubmed:25209871] |
European Journal of Cancer Supplements | A91: Endogenous inhibitors of cysteine proteases and preform of cathepsin B in cancer of reproductive system [Science: Article] |
Annals of Clinical Biochemistry | Diagnostic specificity and sensitivity of PIVKAII, GP3, CSTB, SCCA1 and HGF for the diagnosis of hepatocellular carcinoma in patients with alcoholic liver cirrhosis. [pubmed:28766361] |
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