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内皮抑素(ES)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Endostatin (ES)

  • 内皮抑素(ES)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 内皮抑素(ES)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对内皮抑素(ES)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
RPA542Ra01 内皮抑素(ES)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
CEA542Ra 内皮抑素(ES)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA542Ra 内皮抑素(ES)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA542Ra11 内皮抑素(ES)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Cancer Immunology Endostatin gene therapy enhances the efficacy of IL-2 in suppressing metastatic renal cell carcinoma in mice [SpringerLink: u38777121t6660l5]
Journal of Fluoresence Erythrocyte Protoporphyrin Fluorescence as a Biomarker for Monitoring Antiangiogenic Cancer Therapy [SpringerLink: 4v306130302257w0]
The Journal of Gene Medicine Endostatin- and interleukin-2-expressing retroviral bicistronic vector for gene therapy of metastatic renal cell carcinoma [Wiley: source]
Biomedicine & Pharmacotherapy Endostatin neoadjuvant gene therapy extends survival in an orthotopic metastatic mouse model of renal cell carcinoma [ScienceDirect: S075333221100151X]
Science Alert Antiangiogenic Activities of Cinnamon, Black and Green Tea Extracts on Experimentally Induced Breast Cancer in Rats [Scialert: Source]
The Journal of Immunology Inflammation Induced by MMP-9 Enhances Tumor Regression of Experimental Breast Cancer [PubMed: PMC3619527]
Prostaglandins & Other Lipid Mediators Promotion of adipogenesis by an EP2 receptor agonist via stimulation of angiogenesis in pulmonary emphysema [Pubmed:24911647]
American Journal of Physiology - Heart and Circulatory Physiology Endostatin and kidney fibrosis in aging: a case for antagonistic pleiotropy? [Source]
shock Angiogenesis inhibitor endostatin protects mice with sepsis from multiple organ dysfunction syndrome [PubMed: 26125086]
Clin Rev Allergy Immunol Endothelial Dysfunction and Nailfold Videocapillaroscopy Pattern as Predictors of Digital Ulcers in Systemic Sclerosis: a Cohort Study and Review of the Literature [PubMed: 26142066]
Angiologia e Cirurgia Vascular Peripheral vasculopathy in Raynaud phenomenon: Vascular disease biomarkers [science:S1646706X16300052]
Clinical Rheumatology Impaired angiogenesis as a feature of digital ulcers in systemic sclerosis [Pubmed:26920752]
Blood biomarker for early blood brain barrier disruption in ischemic stroke [patent:US20170199205A1]
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