内皮抑素(ES)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Endostatin (ES)
- 编号KSA542Ra11
- 物种Rattus norvegicus (Rat,大鼠) 相同的名称,不同的物种。
- 试剂内容 包被抗体、生物素标记的竞争物、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理竞争抑制酶联免疫吸附试验法测抗原
- 可检测范围1.23-100ng/mL
- 可适灵敏度0.54ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 8208 ¥ 13680 ¥ 24624 ¥ 47880 ¥ 82080
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对内皮抑素(ES)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Rattus norvegicus (Rat,大鼠) | 应用(仅供研究使用,不用于临床诊断!) |
RPA542Ra01 | 内皮抑素(ES)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
CEA542Ra | 内皮抑素(ES)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA542Ra | 内皮抑素(ES)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSA542Ra11 | 内皮抑素(ES)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
Cancer Immunology | Endostatin gene therapy enhances the efficacy of IL-2 in suppressing metastatic renal cell carcinoma in mice [SpringerLink: u38777121t6660l5] |
Journal of Fluoresence | Erythrocyte Protoporphyrin Fluorescence as a Biomarker for Monitoring Antiangiogenic Cancer Therapy [SpringerLink: 4v306130302257w0] |
The Journal of Gene Medicine | Endostatin- and interleukin-2-expressing retroviral bicistronic vector for gene therapy of metastatic renal cell carcinoma [Wiley: source] |
Biomedicine & Pharmacotherapy | Endostatin neoadjuvant gene therapy extends survival in an orthotopic metastatic mouse model of renal cell carcinoma [ScienceDirect: S075333221100151X] |
Science Alert | Antiangiogenic Activities of Cinnamon, Black and Green Tea Extracts on Experimentally Induced Breast Cancer in Rats [Scialert: Source] |
The Journal of Immunology | Inflammation Induced by MMP-9 Enhances Tumor Regression of Experimental Breast Cancer [PubMed: PMC3619527] |
Prostaglandins & Other Lipid Mediators | Promotion of adipogenesis by an EP2 receptor agonist via stimulation of angiogenesis in pulmonary emphysema [Pubmed:24911647] |
American Journal of Physiology - Heart and Circulatory Physiology | Endostatin and kidney fibrosis in aging: a case for antagonistic pleiotropy? [Source] |
shock | Angiogenesis inhibitor endostatin protects mice with sepsis from multiple organ dysfunction syndrome [PubMed: 26125086] |
Clin Rev Allergy Immunol | Endothelial Dysfunction and Nailfold Videocapillaroscopy Pattern as Predictors of Digital Ulcers in Systemic Sclerosis: a Cohort Study and Review of the Literature [PubMed: 26142066] |
Angiologia e Cirurgia Vascular | Peripheral vasculopathy in Raynaud phenomenon: Vascular disease biomarkers [science:S1646706X16300052] |
Clinical Rheumatology | Impaired angiogenesis as a feature of digital ulcers in systemic sclerosis [Pubmed:26920752] |
Blood biomarker for early blood brain barrier disruption in ischemic stroke [patent:US20170199205A1] |
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