上皮中性粒细胞激活肽78(ENA78)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Epithelial Neutrophil Activating Peptide 78 (ENA78)
CXCL5; SCYB5; Chemokine C-X-C-Motif Ligand 5; Small Inducible Cytokine Subfamily B(Cys-X-Cys),Member 5; Neutrophil-activating peptide ENA-78
- 编号KSA860Mu01
- 物种Mus musculus (Mouse,小鼠) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围62.5-4000pg/mL
- 可适灵敏度25.7pg/mL
- 应用自己动手制作ELISA试剂盒的主要材料
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 6998 ¥ 11664 ¥ 20995 ¥ 40824 ¥ 69984
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对上皮中性粒细胞激活肽78(ENA78)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Mus musculus (Mouse,小鼠) | 应用(仅供研究使用,不用于临床诊断!) |
APA860Mu02 | 上皮中性粒细胞激活肽78(ENA78)活性蛋白 | Cell culture; Activity Assays. |
RPA860Mu02 | 上皮中性粒细胞激活肽78(ENA78)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
EPA860Mu61 | 上皮中性粒细胞激活肽78(ENA78)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
EPA860Mu63 | 上皮中性粒细胞激活肽78(ENA78)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA860Mu61 | 上皮中性粒细胞激活肽78(ENA78)活性蛋白 | Cell culture; Activity Assays. |
RPA860Mu01 | 上皮中性粒细胞激活肽78(ENA78)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA860Mu02 | 上皮中性粒细胞激活肽78(ENA78)多克隆抗体 | WB; IHC; ICC; IP. |
PAA860Mu01 | 上皮中性粒细胞激活肽78(ENA78)多克隆抗体 | WB; IHC; ICC; IP. |
LAA860Mu81 | 上皮中性粒细胞激活肽78(ENA78)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
LAA860Mu71 | 上皮中性粒细胞激活肽78(ENA78)多克隆抗体(生物素标记) | WB; IHC; ICC. |
SEA860Mu | 上皮中性粒细胞激活肽78(ENA78)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
AEA860Mu | 抗上皮中性粒细胞激活肽78抗体(Anti-ENA78)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
SCA860Mu | 上皮中性粒细胞激活肽78(ENA78)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMA860Mu | 上皮中性粒细胞激活肽78(ENA78)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSA860Mu01 | 上皮中性粒细胞激活肽78(ENA78)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
杂志 | 参考文献 |
Journal of Biochemical and Molecular Toxicology | Ameliorative Effects of Curcumin on Fibrinogen-Like Protein-2 Gene Expression, Some Oxido-Inflammatory and Apoptotic Markers in a Rat Model of l-Arginine-Induced Acute Pancreatitis. [Pubmed:26862043] |
Journal of Biochemical and Molecular Toxicology | Ameliorative Effects of Curcumin on Fibrinogen-Like Protein-2 Gene Expression, Some Oxido-Inflammatory and Apoptotic Markers in a Rat Model of l-Arginine-Induced Acute Pancreatitis [pubmed:26862043] |
OncoTargets and Therapy | The clinical significance of CXCL5 in non-small cell lung cancer. [pubmed:29200871] |
Biochimie | Activated CXCL5-CXCR2 axis promotes the migration, invasion and EMT of papillary thyroid carcinoma cells via modulation of β-catenin pathway [Pubmed:29471001] |
Cancer Biology & Therapy | Activation of CXCL5-CXCR2 axis promotes proliferation and accelerates G1 to S phase transition of papillary thyroid carcinoma cells and activates JNK and p38 … [Pubmed: 30404567] |
Respiratory Research | Phospholipase Cε plays a crucial role in neutrophilic inflammation accompanying acute lung injury through augmentation of CXC chemokine production from … [Pubmed: 30634975] |
American Journal of Pathology | Interleukin17–CXCR2 axis facilitates breast cancer progression by up-regulating neutrophil recruitment [Pubmed: 31654638] |
Bioactive Materials | Secretions from hypochlorous acid-treated tumor cells delivered in a melittin hydrogel potentiate cancer immunotherapy [34820587] |
Am J Cancer Res | Differential expression profile of CXC-receptor-2 ligands as potential biomarkers in pancreatic ductal adenocarcinoma [Pubmed:35141005] |
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