雌激素受体β(ERb)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Estrogen Receptor Beta (ERb)
ER-B; ESRB; ESTRB; NR3A2; NR3-A2; ESR2; Estrogen Receptor 2; Nuclear Receptor Subfamily 3,Group A,Member 2
- 编号KSA437Hu01
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.312-20ng/mL
- 可适灵敏度0.117ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 6804 ¥ 11340 ¥ 20412 ¥ 39690 ¥ 68040
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对雌激素受体β(ERb)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Homo sapiens (Human,人) | 应用(仅供研究使用,不用于临床诊断!) |
RPA437Hu01 | 雌激素受体β(ERb)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA437Hu01 | 雌激素受体β(ERb)多克隆抗体 | WB; IHC; ICC; IP. |
LAA437Hu71 | 雌激素受体β(ERb)多克隆抗体(生物素标记) | WB; IHC; ICC. |
LAA437Hu81 | 雌激素受体β(ERb)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
FAA437Hu11 | 抗雌激素受体β(ERb)多克隆抗体(标记) | FCM |
MAA437Hu22 | 雌激素受体β(ERb)单克隆抗体 | WB; IHC; ICC; IP. |
MAA437Hu21 | 雌激素受体β(ERb)单克隆抗体 | WB; IHC; ICC; IP. |
SEA437Hu | 雌激素受体β(ERb)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA437Hu | 雌激素受体β(ERb)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMA437Hu | 雌激素受体β(ERb)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSA437Hu01 | 雌激素受体β(ERb)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
The Journal of Steroid Biochemistry and Molecular Biology | Neuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR30 signalling [Pubmed:24846829] |
Biochem Biophys Res Commun | Differential expression of estrogen receptor α and β isoforms in multiple and solitary leiomyomas [PubMed: 26529545] |
Mol Neurobiol | Selective Aryl Hydrocarbon Receptor Modulator 3,3′-Diindolylmethane Impairs AhR and ARNT Signaling and Protects Mouse Neuronal Cells Against Hypoxia [PubMed: 26476840] |
J Steroid Biochem Mol Biol | Neuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR31 signalling [PubMed: 24846830] |
Journal of Environmental Sciences | Bisphenol A exposure alters release of immune and developmental modulators and expression of estrogen receptors in human fetal lung fibroblasts [science:S1001074216300924] |
Biol Open. | Involvement of BDNF/TrkB and ERK/CREB axes in nitroglycerin-induced rat migraine and effects of estrogen on these signals in the migraine. [pubmed:27875242] |
Biomedicine & Pharmacotherapy | Postmenopausal osteoporosis is associated with the regulation of SP, CGRP, VIP, and NPY [Pubmed:29807224] |
Journal of Applied Pharmaceutical Science | Physical Signs of Illness, Liver Functions and Reproductive Parameters of Female Rats Supplemented with Melaleuca cajuputi Methanolic Extract [Pbulication:325682424] |
Carleton University Research Virtual Environment | In Vitro Study of Mechanisms Underlying the Developmental Effects of Bisphenol A Using Human Fetal Lung Fibroblasts [] |
Life Sciences | MiR-145 regulates steroidogenesis in mouse primary granulosa cells through targeting Crkl [34273377] |
Cardiometry | Modifying effect of obesity on the content of sex hormones and their receptors in endometrial adenocarcinoma and its surrounding tissue [] |
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