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促性腺激素释放激素(GnRH)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Gonadotropin Releasing Hormone (GnRH)

GNRH1; GRH; LNRH; LHRH; LH-RH I; Luliberin; Luteinizing-Hormone Releasing Hormone; Progonadoliberin-1; Gonadoliberin I; Gonadorelin; GnRH-associated peptide 1

  • 促性腺激素释放激素(GnRH)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 促性腺激素释放激素(GnRH)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对促性腺激素释放激素(GnRH)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种：Rattus norvegicus (Rat，大鼠) 应用(仅供研究使用，不用于临床诊断！)
RPA843Ra01 促性腺激素释放激素(GnRH)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA843Ra01 促性腺激素释放激素(GnRH)多克隆抗体 WB; IHC; ICC; IP.
LAA843Ra81 促性腺激素释放激素(GnRH)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA843Ra71 促性腺激素释放激素(GnRH)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA843Ra21 促性腺激素释放激素(GnRH)单克隆抗体 WB; IHC; ICC; IP.
CEA843Ra 促性腺激素释放激素(GnRH)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA843Ra 促性腺激素释放激素(GnRH)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA843Ra11 促性腺激素释放激素(GnRH)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Mol Pharmacol. Maternal exposure to dioxin imprints sexual immaturity of the pups through fixing the status of the reduced expression of hypothalamic gonadotropin-releasing … [Pubmed:24132183]
Journal of Sports Science & Medicine Heavy Resistance Training and Supplementation With the Alleged Testosterone Booster Nmda has No Effect on Body Composition, Muscle Performance, and Serum Hormones Associated With the Hypothalamo-Pituitary-Gonadal Axis in Resistance-Trained Males [Pubmed:24570624]
Environmental Research Perfluorooctane sulfonate effects on the reproductive axis in adult male rats [Pubmed:25171141]
Toxicology Letters Possible role of serotonin and neuropeptide Y on the disruption of the reproductive axis activity by perfluorooctane sulfonate [Pubmed:25623392]
Toxicol Sci Chronic exposure of female mice to an environmental level of perfluorooctane sulfonate suppresses estrogen synthesis through reduced histone H3K14 acetylation of the StAR promoter leading to deficits in follicular development and ovulation [PubMed: 26358002]
Int J Mol Sci. Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in Mice [Pubmed:26771609]
Oral Presentations OP0019-HPR Effectiveness of A Progressive Resistance Strength Program on Hand Osteoarthritis: A Randomised Controlled Trial [60.1.abstract]
Oral Presentations OP0021-HPR Sleep Disorders in Patients with Rheumatoid Arthritis and Relationship with Disease Activity [60.3.abstract]
Toxicological Sciences Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring [pubmed:27803384]
Chronobiology of the immune reponse OP0020 The Association of Gonadotropin-Releasing Hormone and Cytokines in Rheumatoid Arthritis [content:75]
Environmental Science & Technology Pyrethroid Insecticide Cypermethrin Accelerates Pubertal Onset in Male Mice via Disrupting Hypothalamic-Pituitary-Gonadal Axis [pubmed:28731686]
Toxicological Sciences Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons [10.1093:toxsci]
GENES Nectin-like molecule 2, a necessary sexual maturation regulator, participates in congenital hypogonadotropic hypogonadism [Pubmed: 32535046]
BioMed Research International Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress [Pubmed: 32685488]
JOURNAL OF ETHNOPHARMACOLOGY Zihuai recipe alleviates cyclophosphamide-induced diminished ovarian reserve via suppressing PI3K/AKT-mediated apoptosis [33422655]
J Virol Zika virus infection in the ovary induces continuously elevated progesterone level and compromises conception in interferon α/β receptor-deficient mice [34730391]
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