血红蛋白(HB)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Hemoglobin (HB)
Hgb; Haemoglobin; Heterotetramer(αβ)2
- 编号KSB409Ra11
- 物种Rattus norvegicus (Rat,大鼠) 相同的名称,不同的物种。
- 试剂内容 包被抗体、生物素标记的竞争物、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, erythrocyte lysates, cell culture supernates and other biological fluids.
- 可适用原理竞争抑制酶联免疫吸附试验法测抗原
- 可检测范围6.17-500ug/mL
- 可适灵敏度2.59ug/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 7798 ¥ 12996 ¥ 23393 ¥ 45486 ¥ 77976
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对血红蛋白(HB)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Rattus norvegicus (Rat,大鼠) | 应用(仅供研究使用,不用于临床诊断!) |
NPB409Ra01 | 血红蛋白(HB)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB409Ra01 | 血红蛋白(HB)多克隆抗体 | WB; IHC; ICC; IP. |
LAB409Ra71 | 血红蛋白(HB)多克隆抗体(生物素标记) | WB; IHC; ICC. |
CEB409Ra | 血红蛋白(HB)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB409Ra | 血红蛋白(HB)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSB409Ra11 | 血红蛋白(HB)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology | Effects of ectoine on behavioural, physiological and biochemical parameters of Daphnia magna [Pubmed:25460046] |
PLoS One. | Comparative Proteomics Identifies Host Immune System Proteins Affected by Infection with Mycobacterium bovis [Pubmed:27027307] |
Parasites & Vectors | The intracellular bacterium Anaplasma phagocytophilum selectively manipulates the levels of vertebrate host proteins in the tick vector Ixodes scapularis. [pubmed:27561965] |
Proteomics. Clinical Applications | Elevated levels of protein AMBP in cerebrospinal fluid of women with preeclampsia compared to normotensive pregnant women. [pubmed:27615121] |
Molecular nutrition & food research | Chicory inulin ameliorates type 2 diabetes mellitus and suppresses JNK and MAPK pathways in vivo and in vitro [pubmed:28105758] |
Frontiers in Immunology | Oxidized Hemoglobin Is Antigenic and Immunogenic in Lupus [pubmed:28694810] |
新規人工酸素運搬体HemoAct を用いた脳虚血再灌流障害治療開発に関する研究 [:] | |
Acta Neuropathologica Communications | Mural cell-derived laminin-α5 plays a detrimental role in ischemic stroke [Pubmed: 30777135] |
Angiogenesis | Decylubiquinone suppresses breast cancer growth and metastasis by inhibiting angiogenesis via the ROS/p53/BAI1 signaling pathway [Pubmed: 32020421] |
Food Bioscience | Water-extractable zinc protoporphyrin IX in Parma ham predominantly exists as complexes with hemoglobin and myoglobin [] |
Photochem Photobiol | Effects of Human Serum Albumin on the Fluorescence Intensity and Tumor Imaging Properties of IR‐780 Dye [34687567] |
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