低氧诱导因子2α(HIF2a)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Hypoxia Inducible Factor 2 Alpha (HIF2a)
EPAS1; HLF; MOP2; PASD2; BHLHE73; Endothelial PAS Domain 1; HIF-1 Alpha-Like Factor; Basic-helix-loop-helix-PAS protein MOP2; Class E basic helix-loop-helix protein 73
- 编号KSD466Ra01
- 物种Rattus norvegicus (Rat,大鼠) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围78-5000pg/mL
- 可适灵敏度29pg/mL
- 应用Main materials for "Do It(ELISA Kit) Yourself"
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 8208 ¥ 13680 ¥ 24624 ¥ 47880 ¥ 82080
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特异性
试剂盒中的抗体对低氧诱导因子2α(HIF2a)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Rattus norvegicus (Rat,大鼠) | 应用(仅供研究使用,不用于临床诊断!) |
RPD466Ra01 | 低氧诱导因子2α(HIF2a)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAD466Ra01 | 低氧诱导因子2α(HIF2a)多克隆抗体 | WB; IHC; ICC; IP. |
LAD466Ra71 | 低氧诱导因子2α(HIF2a)多克隆抗体(生物素标记) | WB; IHC; ICC. |
MAD466Ra21 | 低氧诱导因子2α(HIF2a)单克隆抗体 | WB; IHC; ICC; IP. |
SED466Ra | 低氧诱导因子2α(HIF2a)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMD466Ra | 低氧诱导因子2α(HIF2a)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSD466Ra01 | 低氧诱导因子2α(HIF2a)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It(ELISA Kit) Yourself" |
参考文献
杂志 | 参考文献 |
Neuro Oncol | Combination viroimmunotherapy with checkpoint inhibition to treat glioma, based on location-specific tumor profiling [PubMed: 26409567] |
Clinical Trials in Degenerative Diseases | Correlating oxidative stress-related factors with bone metabolic markers in older adult male patients exhibiting degenerative osteoporosis in the high-altitude hypoxic area of China: study protocol for a non-randomized controlled trial [issn:2542-3975] |
Journal of Physiological Anthropology | Telomere elongation protects heart and lung tissue cells from fatal damage in rats exposed to severe hypoxia [Pubmed:29454386] |
Oxidative damage and iron dyshomeostasis in thalassemic patients [] | |
Neuropsychiatric Disease and Treatment | The Association Between Hypoxia Improvement and Electroconvulsive Therapy for Major Depressive Disorder [34588778] |
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