胰岛素样生长因子结合蛋白3(IGFBP3)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Insulin Like Growth Factor Binding Protein 3 (IGFBP3)
BP53; IBP3; Growth Hormone-Dependent Binding Protein; Acid Stable Subunit Of The 140 K IGF Complex
- 编号KSA054Po01
- 物种Sus scrofa; Porcine (Pig,猪) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.312-20ng/mL
- 可适灵敏度0.138ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 8165 ¥ 13608 ¥ 24494 ¥ 47628 ¥ 81648
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特异性
试剂盒中的抗体对胰岛素样生长因子结合蛋白3(IGFBP3)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Sus scrofa; Porcine (Pig,猪) | 应用(仅供研究使用,不用于临床诊断!) |
RPA054Po01 | 胰岛素样生长因子结合蛋白3(IGFBP3)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA054Po01 | 胰岛素样生长因子结合蛋白3(IGFBP3)多克隆抗体 | WB; IHC; ICC; IP. |
MAA054Po21 | 胰岛素样生长因子结合蛋白3(IGFBP3)单克隆抗体 | WB; IHC; ICC; IP. |
SEA054Po | 胰岛素样生长因子结合蛋白3(IGFBP3)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA054Po | 胰岛素样生长因子结合蛋白3(IGFBP3)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSA054Po01 | 胰岛素样生长因子结合蛋白3(IGFBP3)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
Molecular Biology Reports | Characterization of the specific and sustained GH1 expression induced by rAAV2/1 in normal adult male rats [PubMed: 20204528] |
Endocrinology | Role of IGFBP-3 in the Regulation of β-Cell Mass during Obesity: Adipose Tissue/β-Cell Cross Talk [Endo: source] |
Nutrition and Cancer | A Pilot Study Comparing the Effect of Flaxseed, Aromatase Inhibitor, and the Combination on Breast Tumor Biomarkers [Tandfonline: Source] |
Dig Dis Sci | The Protective Effects of Exclusive Enteral Nutrition Formulas on Growth Factor Expression and the Proximal Tibial Epiphyseal Growth Plate in a TNBS-Induced IBD Rat Model [PubMed: 25708898] |
Animal. | Effects of a dietary crude fibre concentrate on growth in weaned piglets. [pubmed:28318464] |
PLoS One | Altered insulin-like growth factor-2 signaling is associated with psychopathology and cognitive deficits in patients with schizophrenia [Pubmed: 32191705] |
Int J Environ Res Public Health | Association of Salivary IGF and IGF/IGFBP-3 Molar Ratio with Cervical Vertebral Maturation Stages from Pre-Adolescent to Post-Adolescent Transition Period—A … [Pubmed:35564566] |
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