白介素1受体拮抗剂(IL1RA)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Interleukin 1 Receptor Antagonist (IL1RA)
IL1RN; IL1-RA; ICIL1RA; IL1ra3; IL1F3; IRAP;IL-1RA; Interleukin-1 Family Member 3; IL1 inhibitor; Anakinra
- 编号KSA223Po01
- 物种Sus scrofa; Porcine (Pig,猪) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围31.25-2000pg/mL
- 可适灵敏度12.4pg/mL
- 应用自己动手制作ELISA试剂盒的主要材料
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 7308 ¥ 12180 ¥ 21924 ¥ 42630 ¥ 73080
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特异性
试剂盒中的抗体对白介素1受体拮抗剂(IL1RA)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Sus scrofa; Porcine (Pig,猪) | 应用(仅供研究使用,不用于临床诊断!) |
RPA223Po01 | 白介素1受体拮抗剂(IL1RA)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA223Po01 | 白介素1受体拮抗剂(IL1RA)多克隆抗体 | WB; IHC; ICC; IP. |
LAA223Po81 | 白介素1受体拮抗剂(IL1RA)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
LAA223Po71 | 白介素1受体拮抗剂(IL1RA)多克隆抗体(生物素标记) | WB; IHC; ICC. |
MAA223Po22 | 白介素1受体拮抗剂(IL1RA)单克隆抗体 | WB; IHC; ICC; IP. |
KSA223Po01 | 白介素1受体拮抗剂(IL1RA)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
杂志 | 参考文献 |
The Veterinary Journal | Mucosal imbalance of interleukin-1β and interleukin-1 receptor antagonist in canine inflammatory bowel disease [ScienceDirect: S1090023312000962] |
Daily quercetin supplementation over a period of two weeks results in a moderate accumulation of total plasma flavonols in horses [Researchgate:Source] | |
Journal of Equine Veterinary Science | Synovial Fluid and Serum Concentrations of Interleukin-1 Receptor Antagonist and Interleukin-1 [Science: Article] |
Journal of Equine Veterinary Science | A Two-Week Quercetin Supplementation in Horses Results in Moderate Accumulation of Plasma Flavonol Concentrations [Science: Article] |
Scientific publications in peer reviewed journals | Evidence-based review of efficacy and adverse effects of joint medication and evaluation of synovial fluid and serum markers for osteoarthritis in the horse [receive:101658] |
Multiple Sclerosis and Related Disorders | Interleukin 1 receptor antagonist and 2′-5′-oligoadenylate synthetase-like molecules as novel biomarkers for multiple sclerosis patients in Bahrain [pubmed:29141788] |
Advances in Medical Sciences | A similar pro/anti-inflammatory cytokine balance is present in the airways of competitive athletes and non-exercising asthmatics [pubmed:28822267] |
Archives of Medical Research | Winter ambient training conditions are associated with increased bronchial hyperreactivity and with shifts in serum innate immunity proteins in young … [Pubmed:29379533] |
Journal of Veterinary Internal Medicine | Effect of interleukin‐1β on occludin mRNA expression in the duodenal and colonic mucosa of dogs with inflammatory bowel disease [Pubmed:29572935] |
Frontiers in Pharmacology | M10, a Myricetin-3-ObD-Lactose Sodium Salt, Prevents Ulcerative Colitis Through Inhibiting Necroptosis in Mice [Pubmed: 33041798] |
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