白介素15(IL15)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Interleukin 15 (IL15)
- 编号KSA061Ra01
- 物种Rattus norvegicus (Rat,大鼠) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围15.6-1000pg/mL
- 可适灵敏度6.0pg/mL
- 应用自己动手制作ELISA试剂盒的主要材料
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 7387 ¥ 12312 ¥ 22162 ¥ 43092 ¥ 73872
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对白介素15(IL15)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Rattus norvegicus (Rat,大鼠) | 应用(仅供研究使用,不用于临床诊断!) |
RPA061Ra01 | 白介素15(IL15)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA061Ra01 | 白介素15(IL15)多克隆抗体 | WB; IHC; ICC; IP. |
MAA061Ra21 | 白介素15(IL15)单克隆抗体 | WB; IHC; ICC; IP. |
MAA061Ra22 | 白介素15(IL15)单克隆抗体 | WB; IHC; ICC; IP. |
LAA061Ra72 | 白介素15(IL15)单克隆抗体(生物素标记) | WB; IHC; ICC. |
SEA061Ra | 白介素15(IL15)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA061Ra | 白介素15(IL15)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
PSA061Ra01 | 白介素15(IL15)抗体对 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSA061Ra01 | 白介素15(IL15)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
杂志 | 参考文献 |
Endocrine | Expression of interleukin-15 and inflammatory cytokines in skeletal muscles of STZ-induced diabetic rats: effect of resistance exercise training [Pubmed: 24006180] |
Endocrine | Time course of IL-15 expression after acute resistance exercise in trained rats: effect of diabetes and skeletal muscle phenotype [Pubmed:25522723] |
Med Sci Moni | miR-15b is Downregulated in Myasthenia Gravis Patients and Directly Regulates the Expression of Interleukin-15 (IL-15) in Experimental Myasthenia Gravis Mice [PubMed: 26087886] |
Exercise physiology | Effects of intrinsic aerobic capacity, aging and physical activity on interleukin-15 protein level in serum and skeletal muscle [Dspace: Handle] |
Biotechnology Letters | Novel adjuvant for immunization against tuberculosis: DNA vaccine expressing Mycobacterium tuberculosis antigen 85A and interleukin-15 fusion product elicits strong immune responses in mice [s10529-017-2342-1] |
Journal of Controlled Release | Interleukin-15 and cisplatin co-encapsulated thermosensitive polypeptide hydrogels for combined immuno-chemotherapy. [pubmed:28408199] |
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