白介素17F(IL17F)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Interleukin 17F (IL17F)
ML-1
- 编号KSB955Hu01
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围7.8-500pg/mL
- 可适灵敏度2.7pg/mL
- 应用自己动手制作ELISA试剂盒的主要材料
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 7182 ¥ 11970 ¥ 21546 ¥ 41895 ¥ 71820
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对白介素17F(IL17F)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Homo sapiens (Human,人) | 应用(仅供研究使用,不用于临床诊断!) |
APB955Hu61 | 白介素17F(IL17F)活性蛋白 | Cell culture; Activity Assays. |
EPB955Hu61 | 白介素17F(IL17F)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPB955Hu01 | 白介素17F(IL17F)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APB955Hu01 | 白介素17F(IL17F)活性蛋白 | Cell culture; Activity Assays. |
PAB955Hu01 | 白介素17F(IL17F)多克隆抗体 | WB; IHC; ICC; IP. |
LAB955Hu81 | 白介素17F(IL17F)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
LAB955Hu71 | 白介素17F(IL17F)多克隆抗体(生物素标记) | WB; IHC; ICC. |
MAB955Hu22 | 白介素17F(IL17F)单克隆抗体 | WB; IHC; ICC; IP. |
MAB955Hu21 | 白介素17F(IL17F)单克隆抗体 | WB. |
SEB955Hu | 白介素17F(IL17F)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCB955Hu | 白介素17F(IL17F)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMB955Hu | 白介素17F(IL17F)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSB955Hu01 | 白介素17F(IL17F)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
杂志 | 参考文献 |
Cytokine | Expression of IL-17A and IL-17F in lipopolysaccharide-induced acute lung injury and the counteraction of anisodamine or methylprednisolone [ScienceDirect: S1043466614000040] |
Medical Sciences | Cerebrospinal Fluid (CSF) IL-17A, IL-17F, IL-34 and CXCL-13 Levels in Amyotrophic Lateral Sclerosis (ALS/MND) Patients [] |
Revista da Associacao Medica Brasileira | A comparison of IL-17 and IL-34 concentrations in the cerebrospinal fluid of patients with acute inflammatory demyelinating neuropathy and chronic … [] |
Hum Exp Toxicol | Chrysene accelerates the proceeding of chronic obstructive pulmonary disease with the aggravation of inflammation and apoptosis in cigarette smoke exposed mice [33345606] |
Rev Assoc Med Bras | A comparison of IL-17 and IL-34 concentrations in the cerebrospinal fluid of patients with acute inflammatory demyelinating neuropathy and chronic?¡ [33295414] |
J Periodontol | Cytokine profile in serum and gingival crevicular fluid of children with inflammatory bowel disease: A case‐control study [34730850] |
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