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巨噬细胞移动抑制因子(MIF)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Macrophage Migration Inhibitory Factor (MIF)

GIF; GLIF; MMIF; Glycosylation-Inhibiting Factor; L-dopachrome isomerase; L-dopachrome tautomerase; Phenylpyruvate tautomerase

  • 巨噬细胞移动抑制因子(MIF)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 巨噬细胞移动抑制因子(MIF)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对巨噬细胞移动抑制因子(MIF)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
APA698Hu02 巨噬细胞移动抑制因子(MIF)活性蛋白 Cell culture; Activity Assays.
APA698Hu01 巨噬细胞移动抑制因子(MIF)活性蛋白 Cell culture; Activity Assays.
RPA698Hu02 巨噬细胞移动抑制因子(MIF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA698Hu01 巨噬细胞移动抑制因子(MIF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA698Hu01 巨噬细胞移动抑制因子(MIF)多克隆抗体 WB; IHC; ICC; IP.
PAA698Hu02 巨噬细胞移动抑制因子(MIF)多克隆抗体 WB,IHC
MAA698Hu22 巨噬细胞移动抑制因子(MIF)单克隆抗体 WB; IHC; ICC; IP.
SEA698Hu 巨噬细胞移动抑制因子(MIF)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA698Hu 巨噬细胞移动抑制因子(MIF)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA698Hu01 巨噬细胞移动抑制因子(MIF)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Kidney International Targeted reduction of advanced glycation improves renal function in obesity [PubMed: 21412218]
The Journal of Immunology Macrophage Migration Inhibitory Factor Plays a Role in the Regulation of Microfold (M) Cell-Mediated Transport in the Gut [Jimmunol: 5673]
The Journal of Endocrinology Involvement of exercise-induced macrophage migration inhibitory factor in the prevention of fatty liver disease [PubMed: PMC3757527]
The Journal of Immunology Role of macrophage migration inhibitory factor in the regulatory T cell response of tumor-bearing mice [PubMed: PMC3466372]
Diabetologia Deletion of bone-marrow-derived receptor for AGEs (RAGE) improves renal function in an experimental mouse model of diabetes [Pubmed:24957662]
BioMed Research International The Potential Role of Polymethyl Methacrylate as a New Packaging Material for the Implantable Medical Device in the Bladder [PubMed: 25705692]
Diabetologia Combined NOX1/4 inhibition with GKT137831 in mice provides dose-dependent reno- and atheroprotection even in established micro- and macrovascular disease. [pubmed:28160092]
Life Sciences Protective effect of chlorogenic acid on the inflammatory damage of pancreas and lung in mice with l-arginine-induced pancreatitis [pubmed:28919396]
Scientific Reports Cytokine MIF Enhances Blood-Brain Barrier Permeability: Impact for Therapy in Ischemic Stroke [Pubmed:29335619]
Ocular Immunology and Inflammation Immune Response and Mechanisms of IFN-γ in Administration for Keratomycosis [Pubmed: 30307777]
International Journal of Immunopathology and Pharmacology Effect of voluntary running on expression of myokines in brains of rats with depression [Pubmed: 30834799]
Biomarkers in Heart Failure and Associated Diseases Biomarkers in Heart Failure and Associated Diseases []
Science China-Life Sciences Autophagy mediates the secretion of macrophage migration inhibitory factor from cardiomyocytes upon serum-starvation [Pubmed: 31209799]
Folia Morphologica Beneficial effects of voluntary over forced exercise on skeletal muscle structure and myokines expression [Pubmed: 31802473]
Biomed Pharmacother Deletion of macrophage migration inhibitory factor ameliorates inflammation in mice model severe acute pancreatitis [Pubmed: 32062385]
Sci Rep Association between MIF gene promoter rs755622 and susceptibility to coronary artery disease and inflammatory cytokines in the Chinese Han population [33850223]
Commun Biol Macrophage migration inhibitory factor is overproduced through EGR1 in TET2low resting monocytes [Pubmed:35115654]
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