基质金属蛋白酶10(MMP10)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Matrix Metalloproteinase 10 (MMP10)
STMY2; SL2; Stromelysin 2; Transin-2
- 编号KSA098Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围78.12-5000pg/mL
- 可适灵敏度34pg/mL
- 应用自己动手制作ELISA试剂盒的主要材料
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 6804 ¥ 11340 ¥ 20412 ¥ 39690 ¥ 68040
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对基质金属蛋白酶10(MMP10)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA098Hu01 | 基质金属蛋白酶10(MMP10)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA098Hu01 | 基质金属蛋白酶10(MMP10)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA098Hu71 | 基质金属蛋白酶10(MMP10)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAA098Hu81 | 基质金属蛋白酶10(MMP10)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| MAA098Hu22 | 基质金属蛋白酶10(MMP10)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA098Hu | 基质金属蛋白酶10(MMP10)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA098Hu | 基质金属蛋白酶10(MMP10)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA098Hu | 基质金属蛋白酶10(MMP10)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA098Hu01 | 基质金属蛋白酶10(MMP10)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
| 杂志 | 参考文献 |
| Journal of Cellular and Molecular Medicine | Type-specific dysregulation of matrix metalloproteinases and their tissue inhibitors in end-stage heart failure patients: relationship between MMP-10 and LV remodelling [Wiley: source] |
| PLoS One. | Matrix Metalloproteinase-10 Is Required for Lung Cancer Stem Cell Maintenance, Tumor Initiation and Metastatic Potential [PubMed: PMC3335833] |
| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source] |
| Thromb Haemost. | Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke [Pubmed:24671655] |
| Brain, Behavior, and Immunity | Plasma inflammatory biomarkers for Huntington’s disease patients and mouse model [Pubmed:25266150] |
| Biomedical Materials | In vivo bioengineered ovarian tumors based on collagen, matrigel, alginate and agarose hydrogels: a comparative study [Pubmed:25634132] |
| Brain, Behavior, and Immunity | Plasma inflammatory biomarkers for Huntington’s disease patients and mouse model [PubMed: 25266150] |
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