线粒体开放阅读框12S rRNA-C(MOTS-c)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c)
- 编号KSX132Mu11
- 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
- 试剂内容 包被抗体、生物素标记的竞争物、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理竞争抑制酶联免疫吸附试验法测抗原
- 可检测范围2.47-200ng/mL
- 可适灵敏度0.85ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 9065 ¥ 15108 ¥ 27194 ¥ 52878 ¥ 90648
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对线粒体开放阅读框12S rRNA-C(MOTS-c)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| PAX132Mu01 | 线粒体开放阅读框12S rRNA-C(MOTS-c)多克隆抗体 | WB; IHC; ICC; IP. |
| CEX132Mu | 线粒体开放阅读框12S rRNA-C(MOTS-c)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMX132Mu | 线粒体开放阅读框12S rRNA-C(MOTS-c)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSX132Mu11 | 线粒体开放阅读框12S rRNA-C(MOTS-c)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Pediatric Diabetes | Circulating MOTS‐c levels are decreased in obese male children and adolescents and associated with insulin resistance [Pubmed:29691953] |
| Rapid Commun Mass Spectrom | Development of a mass spectrometry‐based detection method for the mitochondrion‐derived peptide MOTS‐c in plasma samples for doping control purposes [Pubmed: 30394592] |
| DIABETOLOGIA | Adiponectin treatment improves insulin resistance in mice by regulating the expression of the mitochondrial-derived peptide MOTS-c and its response to exercise via … [Pubmed: 32880686] |
| Induction of hyperandrogenism and insulin resistance differentially modulates ferroptosis in uterine and placental tissues of pregnant rats [] | |
| FASEB JOURNAL | Neural cell©\derived plasma exosome protein abnormalities implicate mitochondrial impairment in first episodes of psychosis [33454965] |
| Biochim Biophys Acta Mol Basis Dis | MOTS-c interacts synergistically with exercise intervention to regulate PGC-1¦Á expression, attenuate insulin resistance and enhance glucose metabolism in mice via?¡ [33722744] |
| researchsquare | Evolution of Mitochondrial-Derived Peptides, Humanin and MOTSc and Changes in Insulin Sensitivity During Early Gestation [] |
| Mol Psychiatry | Abnormal levels of mitochondrial proteins in plasma neuronal extracellular vesicles in major depressive disorder [34471251] |
| Pharmacol Res | The Mitochondrial-Derived Peptide MOTS-c Relieves Hyperglycemia and Insulin Resistance in Gestational Diabetes Mellitus [34798268] |
| Ann Neurol | SARS‐CoV‐2 and mitochondrial proteins in neural‐derived exosomes of COVID‐19 [Pubmed:35285072] |
| J Clin Med | Evolution of Mitochondrially Derived Peptides Humanin and MOTSc, and Changes in Insulin Sensitivity during Early Gestation in Women with and without Gestational … [Pubmed:35683389] |
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