甲状旁腺激素(PTH)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Parathyroid Hormone (PTH)
iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin
- 编号KSA866Ra11
- 物种Rattus norvegicus (Rat，大鼠) 相同的名称，不同的物种。
- 试剂内容 包被抗体、生物素标记的竞争物、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理竞争抑制酶联免疫吸附试验法测抗原
- 可检测范围12.35-1,000pg/mL
- 可适灵敏度4.43pg/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 7387 ¥ 12312 ¥ 22162 ¥ 43092 ¥ 73872
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对甲状旁腺激素(PTH)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| APA866Ra01 | 甲状旁腺激素(PTH)活性蛋白 | Cell culture; Activity Assays. |
| RPA866Ra01 | 甲状旁腺激素(PTH)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPA866Ra11 | 甲状旁腺激素(PTH)牛血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| CPA866Ra21 | 甲状旁腺激素(PTH)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| PAA866Ra08 | 甲状旁腺激素(PTH)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA866Ra01 | 甲状旁腺激素(PTH)多克隆抗体 | WB; IHC; ICC; IP. |
| MAA866Ra21 | 甲状旁腺激素(PTH)单克隆抗体 | WB; IHC; ICC; IP. |
| CEA866Ra | 甲状旁腺激素(PTH)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA866Ra | 甲状旁腺激素(PTH)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA866Ra | 甲状旁腺激素(PTH)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA866Ra11 | 甲状旁腺激素(PTH)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Biological trace element research | Elevation of PTH and PTHrp Induced by Excessive Fluoride in Rats on a Calcium-deficient Diet [PubMed: 19915804] |
| Basic nutritional investigation | Effects of duodenal redox status on calcium absorption and related genes expression in high-fat diet–fed mice [ScienceDirect: S0899900709004808] |
| The Korean Journal of Nutrition | Effects of a Low Calcium Diet and Oxalate Intake on Calcium Deposits in Soft Tissues and Bone Metabolism in Ovariectomized Rats [KoreaMed: source] |
| Acta Physiologica Hungarica | Whole body vibration is a safe exercise training method and induces no impaired alterations on rat plasma parameters [Pubmed: 22173025] |
| Toxicology and Industrial Health | The effect of supplementation of calcium, vitamin D, boron, and increased fluoride intake on bone mechanical properties and metabolic hormones in rat [PubMed: 22782709] |
| Indian Journal of Experimental Biology | Effect of consumption of fatty acids, calcium, vitamin D and boron with regular physical activity on bone mechanical properties and corresponding metabolic hormones in rats. [PubMed: 22439438] |
| Plos one | Is Gastrectomy-Induced High Turnover of Bone with Hyperosteoidosis and Increase of Mineralization a Typical Osteomalacia? [PubMed: PMC3679169] |
| Nutr Hosp. | Effect of the “protein diet” and bone tissue. [Pubmed: 24483972] |
| Clinical Cancer Research | Zoledronic acid has differential anti-tumour activity in the pre-and post-menopausal bone microenvironment in vivo [Aacrjournals: Source] |
| Nutricion Hospitalaria | Effect of the “protein diet” and bone tissue [Aulamedica: Source] |
| Academic Journal | Effect of whole body vibration on healthy rat plasma parameters [Ebscohost: Source] |
| J Proteome Res. | Biomarkers identified by urinary metabonomics for noninvasive diagnosis of nutritional rickets [Pubmed:25051233] |
| Medicine (Baltimore) | An Attempt to Evaluate Selected Aspects of “Bone–Fat Axis” Function in Healthy Individuals and Patients With Pancreatic Cancer [PubMed: 26266370] |
| J Nutr Sci Vitaminol (Tokyo) | The Impact of Different Amounts of Calcium Intake on Bone Mass and Arterial Calcification in Ovariectomized Rats [PubMed: 26639847] |
| The Korean Society of Pharmacognosy | Effects of Oryza sativa L. Aleurone Layer Extract on Bone Mineral Density and Bone-related Markers in the Ovariectomized Rat [Article: Hksobf_2015_V46N2_167] |
| J Bone Miner Res | Fat and Sucrose Intake Induces Obesity‐Related Bone Metabolism Disturbances: Kinetic and Reversibility Studies in Growing and Adult Rats [Pubmed:26175082] |
| 7 | Cell-matrix signals specify bone endothelial cells during developmental osteogenesis. [pubmed:28218908] |
| PLOS ONE | Prostaglandin-E2 Mediated Increase in Calcium and Phosphate Excretion in a Mouse Model ofDistal Nephron Salt Wasting. [pubmed:27442254] |
| International Journal of Advanced Research in Biological Sciences | Effects of fatty acids, nutrients and whole body vibration on bone histomorphometry, mechanical properties and metabolic parameters in male rat [10.22192/ijarbs.2017.04.04.018] |
| American Journal of Physiology-Renal Physiology | Chronic nicotine exposure reduces klotho expression and triggers different renal and hemodynamic responses in -haploinsufficient mice [Pubmed:29363324] |
| Tropical Animal Health and Production | Dietary supplementation for Santa Inês hair ewes on pasture at pre-and postpartum periods: dry matter intake, digestibility, milk production, and mineral metabolism [Pubmed:29931604] |
| International Immunopharmacology | CD8+ T lymphocytes enhance the anabolic effect of intermittent parathyroid hormone on cementoblasts [Pubmed: 31679847] |
| ENDOCRINOLOGY | A New Therapeutic Approach Using a Calcilytic (AXT914) for Post-Surgical Hypoparathyroidism in Female Rats [Pubmed: 32852547] |
| Kidney International | Calciprotein particles regulate fibroblast growth factor-23 expression in osteoblasts. [] |
| PREVENTIVE AND/OR THERAPEUTIC AGENT FOR OSTEOGENESIS IMPERFECTA AND OTHER DISEASES [] | |
| Oxid Med Cell Longev | Long-Term Administration of Abacavir and Etravirine Impairs Semen Quality and Alters Redox System and Bone Metabolism in Growing Male Wistar Rats [34373766] |
| Gene Therapy | Correction of a knock-in mouse model of acrodysostosis with gene therapy using a rAAV9-CAG-human PRKAR1A vector [34599290] |
| Cells | Calreticulin Shortage Results in Disturbance of Calcium Storage, Mitochondrial Disease, and Kidney Injury [Pubmed:35456008] |
| African Health Sciences | Fibroblast Growth Factor 23 (FGF 23) and intact parathyroid hormone (iPTH) as markers of mineral bone disease among Nigerians with non-diabetic kidney disease [] |
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