甲状旁腺激素相关蛋白(PTHrP)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Parathyroid Hormone Related Protein (PTHrP)
PTHLH; HHM; PTHR; PLP; Osteostatin; Parathyroid Hormone Like Hormone
- 编号KSA819Ra11
- 物种Rattus norvegicus (Rat,大鼠) 相同的名称,不同的物种。
- 试剂内容 包被抗体、生物素标记的竞争物、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理竞争抑制酶联免疫吸附试验法测抗原
- 可检测范围12.35-1,000pg/mL
- 可适灵敏度4.63pg/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 7387 ¥ 12312 ¥ 22162 ¥ 43092 ¥ 73872
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对甲状旁腺激素相关蛋白(PTHrP)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Rattus norvegicus (Rat,大鼠) | 应用(仅供研究使用,不用于临床诊断!) |
RPA819Ra01 | 甲状旁腺激素相关蛋白(PTHrP)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
CPA819Ra21 | 甲状旁腺激素相关蛋白(PTHrP)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
CPA819Ra22 | 甲状旁腺激素相关蛋白(PTHrP)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
CPA819Ra31 | 甲状旁腺激素相关蛋白(PTHrP)钥孔血蓝蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
PAA819Ra01 | 甲状旁腺激素相关蛋白(PTHrP)多克隆抗体 | WB; IHC; ICC; IP. |
PAA819Ra08 | 甲状旁腺激素相关蛋白(PTHrP)多克隆抗体 | WB; IHC; ICC; IP. |
LAA819Ra71 | 甲状旁腺激素相关蛋白(PTHrP)多克隆抗体(生物素标记) | WB; IHC; ICC. |
LAA819Ra81 | 甲状旁腺激素相关蛋白(PTHrP)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
MAA819Ra24 | 甲状旁腺激素相关蛋白(PTHrP)单克隆抗体 | WB; IHC; ICC; IP. |
MAA819Ra21 | 甲状旁腺激素相关蛋白(PTHrP)单克隆抗体 | WB,IHC |
MAA819Ra22 | 甲状旁腺激素相关蛋白(PTHrP)单克隆抗体 | WB; IHC; ICC; IP. |
MAA819Ra23 | 甲状旁腺激素相关蛋白(PTHrP)单克隆抗体 | WB |
SEA819Ra | 甲状旁腺激素相关蛋白(PTHrP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CEA819Ra | 甲状旁腺激素相关蛋白(PTHrP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA819Ra | 甲状旁腺激素相关蛋白(PTHrP)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSA819Ra11 | 甲状旁腺激素相关蛋白(PTHrP)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
Clinical and Experimental Metastasis | Characterization of a new renal cell carcinoma bone metastasis mouse model [PubMed: 20443133] |
Stem Cells and Development | Temporal activation of β-catenin signaling in the chondrogenic process of mesenchymal stem cells affects the phenotype of the cartilage generated. [PubMed: 22133004] |
International journal of biological sciences | PTHrP Expression in Human MDA-MB-231 Breast Cancer Cells Is Critical for Tumor Growth and Survival and Osteoblast Inhibition [PubMed: PMC3753447] |
Investigational New Drugs | Tetrahydrofurofuran-type lignans inhibit breast cancer-mediated bone destruction by blocking the vicious cycle between cancer cells, osteoblasts and osteoclasts [Pubmed: 23673814] |
Toxicology and Applied Pharmacology | Betulinic acid, a bioactive pentacyclic triterpenoid, inhibits skeletal-related events induced by breast cancer bone metastases and treatment [Pubmed:24463094] |
Arch Gynecol Obstet. | Calcitonin gene-and parathyroid hormone-related peptides in normotensive and preeclamptic pregnancies: a nested case–control study [Pubmed:24943060] |
PLoS One | 15-Deoxy-Δ12, 14-Prostaglandin J2 Inhibits Osteolytic Breast Cancer Bone Metastasis and Estrogen Deficiency-Induced Bone Loss [PubMed: 25859665] |
Endocrinology | A role for the calcitonin receptor to limit bone loss during lactation in female mice by inhibiting osteocytic osteolysis [PubMed: 26135836] |
Tumour Biol | Osteopontin expression in co-cultures of human squamous cell carcinoma-derived cells and osteoblastic cells and its effects on the neoplastic cell phenotype and osteoclastic activation. [Pubmed:27312487] |
35 | Hedgehog signaling in bone regulates whole-body energy metabolism through a bone–adipose endocrine relay mediated by PTHrP and adiponectin [pubmed:27740628] |
Bone | Lactation induces increases in the RANK/RANKL/OPG system in maxillary bone [Pubmed:29382610] |
International Journal of Molecular Sciences | A Diet Rich in Fish Oil and Leucine Ameliorates Hypercalcemia in Tumour-Induced Cachectic Mice [Pubmed: 31600911] |
Journal of Orthopaedic Surgery and Research | Identification of a potential gene target for osteoarthritis based on bioinformatics analyses [Pubmed: 32571421] |
International Immunopharmacology | Parathyroid hormone related protein (PTHrP)-mediated hypercalcemia in malignancy associated with anti-PD-1 immune checkpoint inhibitor treatment and related … [] |
Паратиреоидный гормон-родственный протеин как предиктор развития мочекаменной болезни [] | |
Cell Death Dis | Extracellular vesicles-released parathyroid hormone-related protein from Lewis lung carcinoma induces lipolysis and adipose tissue browning in cancer ¡ [33510128] |
Sci Rep | Effects of whole-body vibration training in a cachectic C26 mouse model [34732809] |
Acta Physiologica | The acute adaptation of renal phosphate transporters in the murine kidney to oral phosphate intake requires multiple signals [Pubmed:35334154] |
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