YY肽(PYY)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Peptide YY (PYY)
PYY1; Peptide tyrosine tyrosine
- 编号KSB067Ra11
- 物种Rattus norvegicus (Rat,大鼠) 相同的名称,不同的物种。
- 试剂内容 包被抗体、生物素标记的竞争物、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理竞争抑制酶联免疫吸附试验法测抗原
- 可检测范围12.35-1000pg/mL
- 可适灵敏度4.71pg/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 7798 ¥ 12996 ¥ 23393 ¥ 45486 ¥ 77976
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对YY肽(PYY)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Rattus norvegicus (Rat,大鼠) | 应用(仅供研究使用,不用于临床诊断!) |
RPB067Ra01 | YY肽(PYY)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
CPB067Ra21 | YY肽(PYY)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
PAB067Ra01 | YY肽(PYY)多克隆抗体 | WB; IHC; ICC; IP. |
MAB067Ra21 | YY肽(PYY)单克隆抗体 | WB; IHC; ICC; IP. |
CEB067Ra | YY肽(PYY)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
MEB067Ra | YY肽(PYY)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB067Ra | YY肽(PYY)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSB067Ra11 | YY肽(PYY)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
International Journal of Sport Studies | The Effects of Concurrent Resistance and Endurance Exercise on Hunger Feelings and PYY in Obese Men [Com:Source] |
Annals of Applied Sport Science | Effect of an Acute Incremental Exercise on Plasma Peptide YY, Neuropeptide Y and IGF-1 Concentrations in Young Athletes [Aassjournal:Source] |
Biomedical Reports | Calprotectin in serum and zonulin in serum and feces are elevated after introduction of a diet with lower carbohydrate content and higher fiber, fat and protein contents. [pubmed:28413639] |
Acta Diabetologica | Effect of lifestyle improvement program on the biomarkersof adiposity, inflammation and gut hormones in overweight/obese Asian Indians with prediabetes [pubmed:28620678] |
Biomedical Reports | Calprotectin in serum and zonulin in serum and feces are elevated after introduction of a diet with lower carbohydrate content and higher fiber, fat and protein contents [10.3892/br.2017.865] |
Journal of Diabetes & Metabolism | High Fiber Fat and Protein Contents Lead to Increased Satiety Reduced Sweet Cravings and Decreased Gastrointestinal Symptoms Independently of Anthropometric Hormonal and Metabolic Factors [10.4172/2155-6156.1000733 ] |
Frontiers in Physiology | Ileal Transposition Surgery Decreases Fat Mass and Improves Glucose Metabolism in Diabetic GK Rats: Possible Involvement of FGF21 [Pubmed:29593555] |
Food and Nutrition Research | Alignments of endocrine, anthropometric, and metabolic parameters in type 2 diabetes after intervention with an Okinawa-based Nordic diet [Pubmed:29599686] |
The Journal of Nutrition | Grape Seed Proanthocyanidin Affects Lipid Metabolism via Changing Gut Microflora and Enhancing Propionate Production in Weaned Pigs [Pubmed: 31175811] |
The Journal of Nutritional Biochemistry | Bile acid receptor TGR5 is critically involved in preference for dietary lipids and obesity [Pubmed: 31812910] |
Arch Toxicol | Serotonin involvement in okadaic acid-induced diarrhoea in vivo [34148100] |
foods | Appetite and Satiety Effects of the Acute and Regular Consumption of Green Coffee Phenols and Green Coffee Phenol/Oat β-Glucan Nutraceuticals in Subjects with … [34828792] |
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