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色素上皮衍生因子(PEDF)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Pigment Epithelium Derived Factor (PEDF)

SERPINF1; EPC1; PIG35; SDF3; Serpin F1; Cell proliferation-inducing gene 35; Serpin Peptidase Inhibitor,Clade F Member 1(Alpha-2 Antiplasmin); Stromal Cell Derived Factor 3

  • 色素上皮衍生因子(PEDF)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 色素上皮衍生因子(PEDF)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对色素上皮衍生因子(PEDF)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种：Rattus norvegicus (Rat，大鼠) 应用(仅供研究使用，不用于临床诊断！)
RPB972Ra01 色素上皮衍生因子(PEDF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB972Ra01 色素上皮衍生因子(PEDF)多克隆抗体 WB; IHC; ICC; IP.
MAB972Ra21 色素上皮衍生因子(PEDF)单克隆抗体 WB; IHC; ICC; IP.
SEB972Ra 色素上皮衍生因子(PEDF)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB972Ra 色素上皮衍生因子(PEDF)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMB972Ra 色素上皮衍生因子(PEDF)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSB972Ra01 色素上皮衍生因子(PEDF)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Molecular Therapy A Therapeutic Strategy for Choroidal Neovascularization Based on Recruitment of Mesenchymal Stem Cells to the Sites of Lesions [Nature: 2010144]
American Journal of Nephrology Urinary Pigment Epithelium-Derived Factor as a Marker of Diabetic Nephropathy [Karger: 314326]
34 Increased expression of pigment epithelium-derived factor in aged mesenchymal stem cells impairs their therapeutic efficacy for attenuating myocardial infarction injury [OxfordJournals: source]
Heart and Education in Heart The change of serum level of pigment epithelium-derived factor in coronary heart disease [Bmj: Source]
Mediators of Inflammation Metformin Inhibits Expression and Secretion of PEDF in Adipocyte and Hepatocyte via Promoting AMPK Phosphorylation [Pubmed: 24288442]
Cardiology. The association study of plasma levels of pigment epithelium-derived factor with acute coronary syndrome in the chinese han population. [Pubmed: 24192856]
Endocrinology Rosiglitazone Inhibits Expression and Secretion of PEDF in Adipose Tissue and Liver of Male SD Rats Via a PPAR-γ Independent Mechanism [Endocrine: Source]
Clinical Science Pigment epithelium-derived factor regulates microvascular permeability through adipose triglyceride lipase in sepsis [PubMed: 25700221]
Molecular Sciences Pigment Epithelium-Derived Factor (PEDF) Protects Osteoblastic Cell Line from Glucocorticoid-Induced Apoptosis via PEDF-R [Pubmed:27187377]
Investigative ophthalmoloey & visual science The Ingenious Interactions Between Macrophages and Functionally Plastic Retinal Pigment Epithelium Cells [articleid:2582822]
Scientific reports VEGF-B promotes recovery of corneal innervations and trophic functions in diabetic mice [pubmed:28091556]
国际眼科杂志 色素上皮衍生因子修饰的人脐带间充质干细胞构建方法 [201707007]
Investigative Ophthalmology & Visual Science PEDF Reduces the Severity of Herpetic Simplex Keratitis in Mice [Pubmed:30025136]
International Eye Science 高海拔地区新生血管性青光眼不同治疗方式的疗效及对房水中PEDF, VEGF 水平的影响 [2018/2/201802020.pdf]
Domestic Animal Endocrinology Lactation-related changes in tissue expression of PEDF in dairy cows [Pubmed:29758402]
Medical Science Monitor Correlation Between Pigment Epithelium-Derived Factor (PEDF) level and Degree of Coronary Angiography and Severity of Coronary Artery Disease in a … [Pubmed:29574467]
Chemosphere Role of pigment epithelium-derived factor (PEDF) on arsenic-induced neuronal apoptosis [Doi: 10.1016/j.chemosphere.2018.10.100]
Biological Trace Element Research Role of Pigment Epithelium-Derived Factor in Arsenic-Induced Vascular Endothelial Dysfunction in a Rat Model [Pubmed: 30392020]
International Urology and Nephrology Increased levels of serum pigment epithelium-derived factor aggravate proteinuria via induction of podocyte actin rearrangement [Pubmed: 30536192]
International Journal of Ophthalmology A novel xeno-free culture system for human retinal pigment epithelium cells [Pubmed: 31024807]
Journal of the American Heart Association Pigment Epithelial‐Derived Factor Deficiency Accelerates Atherosclerosis Development via Promoting Endothelial Fatty Acid Uptake in Mice With Hyperlipidemia [Pubmed: 31711388]
environmental research Pigment epithelium-derived factor (PEDF) ameliorates arsenic-induced vascular endothelial dysfunction in rats and toxicity in endothelial EA. hy926 cells [Pubmed: 32315827]
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES The Expression Levels and Cellular Localization of Pigment Epithelium Derived Factor (PEDF) in Mouse Testis: Its Possible Involvement in the Differentiation of Spermatogonial Cells [33498962]
Hum Cell Long non-coding RNA Rian promotes the expression of tight junction proteins in endothelial cells by regulating perivascular-resident macrophage-like melanocytes ¡­ [33768511]
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