血小板衍生生长因子A(PDGFA)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Platelet Derived Growth Factor Subunit A (PDGFA)
PDGF-A; PDGF1; Platelet Derived Growth Factor Alpha Polypeptide
- 编号KSA528Hu01
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.156-10ng/mL
- 可适灵敏度0.056ng/mL
- 应用自己动手制作ELISA试剂盒的主要材料
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 6804 ¥ 11340 ¥ 20412 ¥ 39690 ¥ 68040
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特异性
试剂盒中的抗体对血小板衍生生长因子A(PDGFA)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Homo sapiens (Human,人) | 应用(仅供研究使用,不用于临床诊断!) |
RPA528Hu01 | 血小板衍生生长因子A(PDGFA)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA528Hu01 | 血小板衍生生长因子A(PDGFA)活性蛋白 | Cell culture; Activity Assays. |
LAA528Hu71 | 血小板衍生生长因子A(PDGFA)多克隆抗体(生物素标记) | WB; IHC; ICC. |
LAA528Hu81 | 血小板衍生生长因子A(PDGFA)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
MAA528Hu22 | 血小板衍生生长因子A(PDGFA)单克隆抗体 | WB; IHC; ICC; IP. |
MAA528Hu21 | 血小板衍生生长因子A(PDGFA)单克隆抗体 | WB; IHC; ICC; IP. |
MAA528Hu23 | 血小板衍生生长因子A(PDGFA)单克隆抗体 | WB; IHC; ICC; IP. |
SEA528Hu | 血小板衍生生长因子A(PDGFA)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA528Hu | 血小板衍生生长因子A(PDGFA)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSA528Hu01 | 血小板衍生生长因子A(PDGFA)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
杂志 | 参考文献 |
Canadian Journal of Physiology and Pharmacology | The effect of midkine on growth factors and oxidative status in an experimental wound model in diabetic and healthy rats [pubmed:28177680] |
International Immunopharmacology | Shikonin alleviates allergic airway remodeling by inhibiting the ERK-NF-κB signaling pathway [pubmed:28521243] |
Open Access Macedonian Journal of Medical Sciences | Low Degree Hyaluronic Acid Crosslinking Inducing the Release of TGF-Β1 in Conditioned Medium of Wharton's Jelly-Derived Stem Cells [] |
Prostaglandins & other Lipid Mediators | Lipoxin A4 inhibited the activation of Hepatic Stellate Cells-T6 cells by modulating profibrotic cytokines and NF-κB signaling pathway [Pubmed: 31698141] |
Ann Agric Environ Med | PDGF-BB homodimer serum level–a good indicator of the severity of alcoholic liver cirrhosis [Pubmed: 32208584] |
Tissue Eng Regen Med | Effect of Leukocyte-Platelet Rich Fibrin (L-PRF) on Tissue Regeneration and Proliferation of Human Gingival Fibroblast Cells Cultured Using a Modified Method [34339025] |
Biomed Research International | Thermal Oscillation Changes the Liquid-Form Autologous Platelet-Rich Plasma into Paste-Like Form [Pubmed:35586817] |
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