降钙素原(PCT)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Procalcitonin (PCT)
Pro-Calcitonin
- 编号KSA689Ga01
- 物种Chicken (Gallus,鸡) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围31.2-2000pg/mL
- 可适灵敏度12.7pg/mL
- 应用自己动手制作ELISA试剂盒的主要材料
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 7434 ¥ 12390 ¥ 22302 ¥ 43365 ¥ 74340
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对降钙素原(PCT)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Chicken (Gallus,鸡) | 应用(仅供研究使用,不用于临床诊断!) |
RPA689Ga01 | 降钙素原(PCT)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA689Ga01 | 降钙素原(PCT)多克隆抗体 | WB; IHC; ICC; IP. |
KSA689Ga01 | 降钙素原(PCT)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
杂志 | 参考文献 |
Scandinavian Journal of Infectious Diseases | Evaluation of neutrophilic CD64, interleukin 10 and procalcitonin as diagnostic markers of early- and late-onset neonatal sepsis [PubMed: 20085423] |
International Journal of Radiation Oncology ? Biology ? Physics | Effects of Berberine Against Radiation-Induced Intestinal Injury in Mice [PubMed: 20637981] |
Ulus Travma Acil Cerrahi Derg | Diagnostic and prognostic value of procalcitonin and phosphorus in acute mesenteric ischemia [PubMed: 21935794] |
Egyptian Journal of Bronchology | Serum procalcitonin and its relation to the outcome of ventilator-associated pneumoniain children [Essbronchology: Source] |
University of Waikato | The Assessment of Vitamin D, Antimicrobial Peptides and Procalcitonin in Bronchiectasis [Waikato:Source] |
Egyptian Journal of Medical Microbiology | Evaluation of the Diagnostic Utility of Fc Gamma Receptor Type 1 (nCD64) and Procalcitonin in Comparison with Conventional Methods for Diagnosis of Early Onset Neonatal Sepsis [Ejmm:Source] |
Nigerian Medical Journal | Efficacy of measuring procalcitonin levels in determination of prognosis and early diagnosis of bacterial resistance in sepsis [Pubmed:25657488] |
Wiley online library | The use and value of procalcitonin in solid organ transplantation [PubMed: 25996831] |
Balkan Med J | Diagnostic Value of Procalcitonin Levels in Acute Mesenteric Ischemia [PubMed: 26185718] |
The Journal of Clinical Endocrinology & Metabolism | Macrocalcitonin is a novel pitfall in the routine of serum calcitonin immunoassay [PubMed: 26647152] |
Journal of Human Hypertension | Procalcitonin and Pentraxin-3: Current biomarkers in inflammation in white coat hypertension [PubMed: 26040439] |
Medical Science Monitor | Variation of Circulating Inflammatory Mediators in Staphylococcus aureus and Escherichia coli BloodstreamInfection. [Pubmed:26772168] |
International Immunopharmacology | Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgery [Pubmed:26802602] |
Pancreatology. | Early prediction of intestinal mucosal barrier function impairment by elevated serum procalcitonin in rats with severe acute pancreatitis. [Pubmed:26804005] |
Acta Medica | Risk Factors of Acute Pancreatitis in Oral Double Balloon Enteroscopy. [pubmed:27638962] |
The Clinical Respiratory Journal | Changes in inflammatory mediators as a result of intermittent hypoxia in obstructive sleep apnea syndrome [pubmed:28960823] |
SHOCK | INTERLEUKIN-1 RECEPTOR 2: A NEW BIOMARKER FOR SEPSIS DIAGNOSIS AND GRAM-NEGATIVE/GRAM-POSITIVE BACTERIAL DIFFERENTIATION [pubmed:27984536] |
Cellular Physiology and Biochemistry | Hepatic Macrophages are the Cell Source of Hepatic Procalcitonin in Acute Liver Failure [Pubmed:29913443] |
Archives of Medical Science | Inflammatory mediators in the diagnosis and treatment of acute pancreatitis: pentraxin-3, procalcitonin and myeloperoxidase [Pubmed:29593801] |
Journal of Thoracic Disease | Expression pattern of soluble triggering receptor expressed on myeloid cells-1 in mice with Acinetobacter baumannii colonization and infection in the lung [Pubmed:29707313] |
Asian Journal of Psychiatry | Procalcitonin and C-reactive protein as peripheral inflammatory markers in antipsychotic drug-free schizophrenia patients [Pubmed:29723719] |
European Review for Medical and Pharmacological Sciences | The predictive value of joint detection of serum amyloid protein A, PCT, and Hs-CRP in the diagnosis and efficacy of neonatal septicemia. [Pubmed: 31298341] |
Medicina | Modestly Elevated Serum Procalcitonin Levels in Patients with Rheumatoid Arthritis Free of Active Infection [Pubmed: 33080909] |
MEDIATORS OF INFLAMMATION | Reevaluation of Lung Injury in TNF-Induced Shock: The Role of the Acid Sphingomyelinase [Pubmed: 32410851] |
Early label-free analysis of mitochondrial redox states by Raman spectroscopy predicts septic outcomes [] | |
Journal of Immunology Research | Moesin Is a Novel Biomarker of Endothelial Injury in Sepsis [33628853] |
Journal of Medical Biochemistry | Circulating nuclear factor-kappa B mediates cancer-associated inflammation in human breast and colon cancer [33776564] |
Surg Endosc | Original contribution: sleeve gastrectomy reduces soluble lectin-like oxidized low-density lipoprotein receptor-1 (sLOX-1) levels in patients with morbid obesity [Pubmed:35044516] |
Bone Res | Procalcitonin is expressed in osteoblasts and limits bone resorption through inhibition of macrophage migration during intermittent PTH treatment [Pubmed:35087025] |
留言咨询