孕酮(PG)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Progesterone (PG)
P4; Pregn-4-Ene-3,20-Dione
- 编号KSA459Ge11
- 物种Pan-species (General,通用) 相同的名称,不同的物种。
- 试剂内容 包被抗体、生物素标记的竞争物、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本serum, plasma and other biological fluids
- 可适用原理竞争抑制酶联免疫吸附试验法测抗原
- 可检测范围1.23-100ng/mL
- 可适灵敏度0.47ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 8597 ¥ 14328 ¥ 25790 ¥ 50148 ¥ 85968
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对孕酮(PG)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Pan-species (General,通用) | 应用(仅供研究使用,不用于临床诊断!) |
CPA459Ge31 | 孕酮(PG)钥孔血蓝蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
CPA459Ge11 | 孕酮(PG)牛血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
CPA459Ge21 | 孕酮(PG)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
CPA459Ge41 | 孕酮(PG)人血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
PAA459Ge01 | 孕酮(PG)多克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
PAA459Ge02 | 孕酮(PG)多克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
LAA459Ge71 | 孕酮(PG)多克隆抗体(生物素标记) | WB; IHC; ICC. |
MAA459Ge24 | 孕酮(PG)单克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
MAA459Ge21 | 孕酮(PG)单克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
MAA459Ge23 | 孕酮(PG)单克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
MAA459Ge22 | 孕酮(PG)单克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
CEA459Ge | 孕酮(PG)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
IEA459Ge | 孕酮(PG)检测试剂盒(酶联免疫吸附试验法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CCA459Ge | 孕酮(PG)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMA459Ge | 孕酮(PG)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSA459Ge11 | 孕酮(PG)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
Fertility and Sterility | Growth differential factor-9 inhibits testosterone production in mouse theca interstitial cells [Pubmed: 23954356] |
Am J Obstet Gynecol. | Prevention of preterm birth by progestational agents: what are the molecular mechanisms? [Pubmed: 23433326] |
Placenta | The role of Sep (O-phosphoserine) tRNA: Sec (selenocysteine) synthase (SEPSECS) in proliferation, apoptosis and hormone secretion of trophoblast cells [Pubmed: 23966103] |
Veterinary Immunology and Immunopathology | Neutrophil gene dynamics and plasma cytokine levels in dairy cattle during peri-implantation period [Pubmed:27090626] |
Clinical and Experimental Hypertension | Assessment of ADMA, estradiol, and progesterone in severe preeclampsia [Pubmed:27152507] |
Scientific Reports | The Reproductive Toxicity of CdSe/ZnS Quantum Dots on the in vivo Ovarian Function and in vitro Fertilization [pubmed:27876896] |
Scientific Reports | Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus [pubmed:27461373] |
33 | Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. [pubmed:28336389] |
Toxicological Sciences | Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring [pubmed:27803384] |
Molecular Neurobiology | Middle-Aged Diabetic Females and Males Present Distinct Susceptibility to Alzheimer Disease-like Pathology. [pubmed:27730513] |
Sci Rep | Exposure of pregnant mice to triclosan impairs placental development and nutrient transport. [pubmed:28322267] |
Interact Cardiovasc Thorac Surg | Acute administration of oestradiol or progesterone in a spinal cord ischaemia–reperfusion model in rats [pubmed:29049608] |
Toxicological Sciences | Exposure of Pregnant Mice to Triclosan Causes Insulin Resistance via Thyroxine Reduction [pubmed:28973666] |
Life Sciences | The anorectic agent, lorcaserin, disturbs estrous cyclicity and produces endometrial hyperplasia without affecting ovarian population in female rats [pubmed:28599864] |
Placenta | Progesterone attenuates hypertension and autoantibody levels to the angiotensin II type 1 receptor in response to elevated cadmium during pregnancy [10.1016:j.placenta.2017.12.004] |
Nanotheranostics | The biocompatibility studies of polymer dots on pregnant mice and fetuses [pubmed:29071192] |
DARU Journal of Pharmaceutical Sciences | Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathway [pubmed:29020999] |
Toxicological Sciences | Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons [10.1093:toxsci] |
Frontiers in Molecular Neuroscience | Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice [Pubmed:29403355] |
Medical Science Monitor | Up-Regulation of Long Noncoding RNA SRA Promotes Cell Growth, Inhibits Cell Apoptosis, and Induces Secretion of Estradiol and Progesterone in Ovarian … [Pubmed:29674607] |
Oncology Letters | Prostaglandin E2 triggers cytochrome P450 17α hydroxylase overexpression via signal transducer and activator of transcription 3 phosphorylation and promotes … [10.3892:ol.2018.9165] |
Biomedicine & Pharmacotherapy | Crocetin attenuates DHT-induced polycystic ovary syndrome in mice via revising kisspeptin neurons [Doi: 10.1016/j.biopha.2018.08.135] |
Journal of Endocrinology | Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome [Pubmed: 29535146] |
Stem Cell Research & Therapy | Endometrial mesenchymal stem cells isolated from menstrual blood repaired epirubicin-induced damage to human ovarian granulosa cells by inhibiting the … [Pubmed: 30606243] |
Journal of Cellular Physiology | Dihydrotestosterone synthesis in the sheep corpus luteum and its potential mechanism in luteal regression [Pubmed: 30671954] |
Experimental and Therapeutic Medicine | Tanshinone IIA attenuates estradiol‑induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary [] |
Experimental Neurology | Reduced serotonin impairs long-term depression in basolateral amygdala complex and causes anxiety-like behaviors in a mouse model of perimenopause [Pubmed: 31377402] |
Molecular reproduction and development | Evidence that downregulation of Wilms' tumor 1 (WT1) is involved in cortical stromal cell differentiation into theca cells in adult bovine ovaries [Pubmed: 31490589] |
Journal of Ovarian Research | Baicalin ameliorates polycystic ovary syndrome through AMP-activated protein kinase [Pubmed: 31722718] |
Chemosphere | Exposure of adult mice to perfluorobutanesulfonate impacts ovarian functions through hypothyroxinemia leading to down-regulation of Akt-mTOR signaling [Pubmed: 31809938] |
Biosensors & Bioelectronics | An antibody-aptamer sandwich cathodic photoelectrochemical biosensor for the detection of progesterone [Pubmed: 32339148] |
JOURNAL OF MOLECULAR NEUROSCIENCE | Serum Progesterone and Testosterone Levels in Schizophrenia Patients at Different Stages of Treatment [Pubmed: 33159671] |
Chemosphere | Prenatal exposure of female mice to perfluorononanoic acid delays pubertal activation of the reproductive endocrine axis through enhanced hepatic FGF21 … [Pubmed: 33131727] |
ARCHIVES OF GYNECOLOGY AND OBSTETRICS | MiRNAs expression profiling of rat ovaries displaying PCOS with insulin resistance [Pubmed: 32757043] |
The Journal of Physiology | Guoqi Liu1, Juan Li2, 3, Peng Cui2, Susanne Lager5, Amanda Nancy Sferruzzi-Perri6, Wei Li1, Xiao-ke Wu1, Yanhua Han1, Mats Brännström7, Linus R Shao2 … [Pubmed: 31206177] |
BioMed Research International | Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress [Pubmed: 32685488] |
DOMESTIC ANIMAL ENDOCRINOLOGY | Wilms' tumor (WT1)(+/-KTS) variants decreases the progesterone secretion of bovine ovarian theca cells [Pubmed: 32739762] |
Journal of Reproductive Immunology | Implantation associated changes in expression profile of indoleamine-2, 3-dioxygenase 1, Th1-Th2 cytokines and interferon-stimulated genes on neutrophils and … [Pubmed: 32846354] |
Iranian Journal of Basic Medical Sciences | Therapeutic effects of organic zinc on reproductive hormones, insulin resistance and mTOR expression as a novel component in a rat model of PCOS [] |
Improving reproductive efficiency through the supplementation of mustard oil, poly-herbal mixture and butyric acid during the periparturient period in Sahiwal … [] | |
Ecotoxicol Environ Safe | A preliminary study on the mechanism of the neurosteroid-mediated ionotropic receptor dysfunction in neurodevelopmental toxicity induced by?¡ [33862428] |
Life Sciences | MiR-145 regulates steroidogenesis in mouse primary granulosa cells through targeting Crkl [34273377] |
Antioxidants (Basel) | Effects of a Nanoencapsulated Moringa Leaf Ethanolic Extract on the Physiology, Metabolism and Reproductive Performance of Rabbit Does during Summer [34439574] |
J Virol | Zika virus infection in the ovary induces continuously elevated progesterone level and compromises conception in interferon α/β receptor-deficient mice [34730391] |
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