转化生长因子β3(TGFb3)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Transforming Growth Factor Beta 3 (TGFb3)
TGF-B3; LAP; Latency-associated peptide
- 编号KSB949Hu01
- 物种Homo sapiens (Human,人) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围15.6-1000pg/mL
- 可适灵敏度5.7pg/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 7182 ¥ 11970 ¥ 21546 ¥ 41895 ¥ 71820
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对转化生长因子β3(TGFb3)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Homo sapiens (Human,人) | 应用(仅供研究使用,不用于临床诊断!) |
RPB949Hu02 | 转化生长因子β3(TGFb3)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APB949Hu02 | 转化生长因子β3(TGFb3)活性蛋白 | Cell culture; Activity Assays. |
RPB949Hu01 | 转化生长因子β3(TGFb3)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APB949Hu01 | 转化生长因子β3(TGFb3)活性蛋白 | Cell culture; Activity Assays. |
PAB949Hu01 | 转化生长因子β3(TGFb3)多克隆抗体 | WB; IHC; ICC; IP. |
PAB949Hu02 | 转化生长因子β3(TGFb3)多克隆抗体 | WB; IHC; ICC; IP. |
LAB949Hu71 | 转化生长因子β3(TGFb3)多克隆抗体(生物素标记) | WB; IHC; ICC. |
MAB949Hu23 | 转化生长因子β3(TGFb3)单克隆抗体 | WB; IHC; ICC; IP. |
RAB949Hu21 | 转化生长因子β3(TGFb3)重组抗体 | WB; IF; ICC; IHC; IP; FCM. |
MAB949Hu22 | 转化生长因子β3(TGFb3)单克隆抗体 | WB |
MAB949Hu24 | 转化生长因子β3(TGFb3)单克隆抗体 | WB; IHC; ICC; IP. |
MAB949Hu21 | 转化生长因子β3(TGFb3)单克隆抗体 | WB; IHC; ICC; IP. |
SEB949Hu | 转化生长因子β3(TGFb3)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCB949Hu | 转化生长因子β3(TGFb3)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMB949Hu | 转化生长因子β3(TGFb3)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
PSB949Hu01 | 转化生长因子β3(TGFb3)抗体对 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSB949Hu01 | 转化生长因子β3(TGFb3)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source] |
J Reprod Immunol | The immunomodulating effect of seminal plasma on T cells [PubMed: 25799173] |
Fertility and Sterility | Influence of vitamin D and transforming growth factor β3 serum concentrations, obesity, and family history on the risk for uterine fibroids [pubmed:27743697] |
Eur J Pharm Biopharm | New scaffolds encapsulating TGF-β3/BMP-7 combinations driving strong chondrogenic differentiation [pubmed:28087378] |
Transforme edici büyüme faktörü-beta-1 nötralizan antikoru ve transforme edici büyüme faktörü-beta-3'ün trakeal darlık gelişimi üzerine etkileri [10.5606/tgkdc.dergisi.2017.14302] | |
Fertility and Sterility | Ulipristal acetate decreases transforming growth factor β3 serum and tumor tissue concentrations in patients with uterine fibroids [Pubmed:29525690] |
Scientific Reports | TGF-β concentrations and activity are down-regulated in the aqueous humor of patients with neovascular age-related macular degeneration [Pubmed:29795291] |
Immunology Letters | The involvement of multifunctional TGF-β and related cytokines in pathogenesis of endometriosis [Pubmed: 30367890] |
Cell Reports | Transforming Growth Factor-β3 Regulates Adipocyte Number in Subcutaneous White Adipose Tissue [Pubmed: 30332637] |
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Temporal TGF-β Supergene Family Signalling Cues Modulating Tissue Morphogenesis: Chondrogenesis within a Muscle Tissue Model? [Pubmed: 32660137] |
Equine Vet J | Limited added value of negative pressure wound therapy compared to calcium alginate dressings for second intention healing in a non©\contaminated and?¡ [34115409] |
AAPS PharmSciTech | Nanoparticles and Nanostructured Films with TGF-β3: Preparation, Characterization, and Efficacy [34378118] |
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