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心肌肌钙蛋白T(TNNT2)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Troponin T Type 2, Cardiac (TNNT2)

CMH2; cTnT; TnTc; Cardiomyopathy,Hypertrophic 2; Cardiac muscle troponin T

  • 心肌肌钙蛋白T(TNNT2)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 心肌肌钙蛋白T(TNNT2)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对心肌肌钙蛋白T(TNNT2)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种：Rattus norvegicus (Rat，大鼠) 应用(仅供研究使用，不用于临床诊断！)
RPD232Ra01 心肌肌钙蛋白T(TNNT2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAD232Ra01 心肌肌钙蛋白T(TNNT2)多克隆抗体 WB; IHC; ICC; IP.
MAD232Ra21 心肌肌钙蛋白T(TNNT2)单克隆抗体 WB; IHC; ICC; IP.
MED232Ra 心肌肌钙蛋白T(TNNT2)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
SED232Ra 心肌肌钙蛋白T(TNNT2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCD232Ra 心肌肌钙蛋白T(TNNT2)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMD232Ra 心肌肌钙蛋白T(TNNT2)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSD232Ra01 心肌肌钙蛋白T(TNNT2)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

参考文献

杂志 参考文献
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Basic & Clinical Pharmacology & Toxicology Cardioprotective Effect of SMND-309, A Novel Derivate of Salvianolic Acid B on Acute Myocardial Infarction in Rats [Wiley: source]
European Journal of Pharmacology Protective roles of Asperosaponin VI, a triterpene saponin isolated from Dipsacus asper Wall on acute myocardial infarction in rats [ScienceDirect: S0014299909009984]
Journal of translational medicine The altered expression profile of microRNAs in cardiopulmonary bypass canine models and the effects of mir-499 on myocardial ischemic reperfusion injury [PubMed: PMC3694448]
Oral Diseases Salivary high‐sensitivity cardiac troponin T levels in patients with acute myocardial infarction [Pubmed: 22834943]
An International Journal of Pharmaceutical Sciences In vivo and in vitro cardioprotective effects of Panax quinquefolium 20 (S)-protopanaxadiol saponins (PQDS), isolated from Panax quinquefolium [Pubmed: 23700796]
Journal of Animal and Feed Sciences, The effects of caloric restriction and age on thyroid hormone signalling in the heart of rats [Pl:Source]
Liver Int. Cardiomyopathy reverses with recovery of liver injury, cholestasis and cholanemia in mouse model of biliary fibrosis [Pubmed:24330504]
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British Journal of Pharmacology Glutathione administration reduces mitochondrial damage and shifts cell death from necrosis to apoptosis in ageing diabetic mice hearts during exercise [Pubmed:25039894]
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EXPERIMENTAL AND THERAPEUTIC MEDICINE Cardioprotective effect of Notch signaling on the development of myocardial infarction complicated by diabetes mellitus [pubmed:29042932]
PLoS One Increases in plasma corin levels following experimental myocardial infarction reflect the severity of ischemic injury [Pubmed: 30192780]
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Journal of Human Kinetics Hormonal Response to Incremental and Continuous Exercise in Cyclists with Left Ventricle Hypertrophy [Pubmed: 32148580]
BIOMEDICINE & PHARMACOTHERAPY Chlorogenic acid prevents acute myocardial infarction in rats by reducing inflammatory damage and oxidative stress [Pubmed: 33022535]
Toxics Norepinephrine Leads to More Cardiopulmonary Toxicities than Epinephrine by Catecholamine Overdose in Rats [Pubmed: 32947820]
Elife Iron derived from autophagy-mediated ferritin degradation induces cardiomyocyte death and heart failure in mice [33526170]
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Experimental and Therapeutic Medicine Hydrogen therapy after resuscitation improves myocardial injury involving inhibition of autophagy in an asphyxial rat model of cardiac arrest [Pubmed:35495584]
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