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线粒体解偶联蛋白2(UCP2)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Mitochondrial Uncoupling Protein 2 (UCP2)

SLC25A8; UCPH; Solute carrier family 25 member 8 ;Uncoupling Protein 2, Mitochondrial

  • 线粒体解偶联蛋白2(UCP2)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 线粒体解偶联蛋白2(UCP2)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对线粒体解偶联蛋白2(UCP2)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
RPC586Mu01 线粒体解偶联蛋白2(UCP2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAC586Mu01 线粒体解偶联蛋白2(UCP2)多克隆抗体 WB; IHC; ICC; IP.
LAC586Mu71 线粒体解偶联蛋白2(UCP2)多克隆抗体(生物素标记) WB; IHC; ICC.
SEC586Mu 线粒体解偶联蛋白2(UCP2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC586Mu 线粒体解偶联蛋白2(UCP2)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSC586Mu01 线粒体解偶联蛋白2(UCP2)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

参考文献

杂志 参考文献
Stem Cells FM19G11 Favors Spinal Cord Injury Regeneration and Stem Cell Self-Renewal by Mitochondrial Uncoupling and Glucose Metabolism Induction [PubMed: 22865656]
Food Research International A 4-week consumption of medium roast and dark roast coffees affects parameters of energy status in healthy subjects [Sciencedirect:S0963996914002403]
The Journal of Maternal-Fetal & Neonatal Medicine The effect of delivery type on uncoupling protein-2 levels [PubMed: 26479203]
Life Sciences Vitamin C and E chronic supplementation differentially affect hepatic insulin signaling in rats [Pubmed:29291420]
REPRODUCTIVE TOXICOLOGY Dose-dependent reproductive toxicity of sodium benzoate in male rats: Inflammation, oxidative stress and apoptosis [Pubmed: 32911041]
Tissue Cell Vitamin B12 ameliorate Tramadol-induced oxidative stress, endocrine imbalance, apoptosis and NO/iNOS/NF-κB expression in Sprague Dawley through regulatory … [34923198]
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