血管内皮生长因子C(VEGFC)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Vascular Endothelial Growth Factor C (VEGFC)
VEGF-C; Flt4-L; VRPL; Vascular Endothelial Growth Factor-Related Protein
- 编号KSA145Mu01
- 物种Mus musculus (Mouse,小鼠) 相同的名称,不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围15.6-1000pg/mL
- 可适灵敏度6.1pg/mL
- 应用自己动手制作ELISA试剂盒的主要材料
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 6998 ¥ 11664 ¥ 20995 ¥ 40824 ¥ 69984
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对血管内皮生长因子C(VEGFC)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Mus musculus (Mouse,小鼠) | 应用(仅供研究使用,不用于临床诊断!) |
RPA145Mu01 | 血管内皮生长因子C(VEGFC)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
EPA145Mu61 | 血管内皮生长因子C(VEGFC)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA145Mu02 | 血管内皮生长因子C(VEGFC)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA145Mu01 | 血管内皮生长因子C(VEGFC)多克隆抗体 | WB; IHC; ICC; IP. |
PAA145Mu02 | 血管内皮生长因子C(VEGFC)多克隆抗体 | WB; IHC; ICC; IP. |
LAA145Mu81 | 血管内皮生长因子C(VEGFC)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
LAA145Mu71 | 血管内皮生长因子C(VEGFC)多克隆抗体(生物素标记) | WB; IHC; ICC. |
MAA145Mu21 | 血管内皮生长因子C(VEGFC)单克隆抗体 | WB |
LAA145Mu72 | 血管内皮生长因子C(VEGFC)单克隆抗体(生物素标记) | WB; IHC; ICC. |
SEA145Mu | 血管内皮生长因子C(VEGFC)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
MEA145Mu | 血管内皮生长因子C(VEGFC)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA145Mu | 血管内皮生长因子C(VEGFC)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
PSA145Mu01 | 血管内皮生长因子C(VEGFC)抗体对 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSA145Mu01 | 血管内皮生长因子C(VEGFC)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
杂志 | 参考文献 |
Journal of Cellular Biochemistry | Alternatively activated RAW264. 7 macrophages enhance tumor lymphangiogenesis in mouse lung adenocarcinoma [PubMed: 19241443] |
genetics and molecular research | Expression of COX-2 and VEGF-C in cholangiocarcinomas at different clinical and pathological stages [PubMed: 26125824] |
Experimental and Therapeutic | Fenofibrate inhibits the expression of VEGFC and VEGFR-3 in retinal pigmental epithelial cells exposed to hypoxia [PubMed: 26622498] |
Am J Pathol | IL-10 Indirectly Regulates Corneal Lymphangiogenesis and Resolution of Inflammation via Macrophages [PubMed: 26608451] |
PLoS One. | Interleukin-6 Induces Vascular Endothelial Growth Factor-C Expression via Src-FAK-STAT3 Signaling in Lymphatic Endothelial Cells. [pubmed:27383632] |
Oncology Letters | RNAi-mediated gene silencing of vascular endothelial growth factor C suppresses growth andinduces apoptosis in mouse breast cancer in vitro and in vivo. [pubmed:27895746] |
Angiogenesis | Characterization of isolated liver sinusoidal endothelial cells for liver bioengineering [Pubmed:29582235] |
Oncology Reports | Effects of diphyllin as a novel V-ATPase inhibitor on TE-1 and ECA-109 cells [Pubmed:29328465] |
LABORATORY INVESTIGATION | Dynamic signature of lymphangiogenesis during acute kidney injury and chronic kidney disease [Pubmed: 31019289] |
Cutaneous and Ocular Toxicology | Isotretinoin does not alter VEGF-A and VEGF-C levels: Do retinoids behave differently in dose-dependent and/or in vivo/in vitro conditions? [Pubmed: 32722957] |
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