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无翅型MMTV整合位点家族成员5A(WNT5A)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Wingless Type MMTV Integration Site Family, Member 5A (WNT5A)

  • 无翅型MMTV整合位点家族成员5A(WNT5A)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 无翅型MMTV整合位点家族成员5A(WNT5A)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对无翅型MMTV整合位点家族成员5A(WNT5A)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种：Rattus norvegicus (Rat，大鼠) 应用(仅供研究使用，不用于临床诊断！)
RPP549Ra01 无翅型MMTV整合位点家族成员5A(WNT5A)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APP549Ra01 无翅型MMTV整合位点家族成员5A(WNT5A)活性蛋白 Cell culture; Activity Assays.
PAP549Ra01 无翅型MMTV整合位点家族成员5A(WNT5A)多克隆抗体 WB; IHC; ICC; IP.
MAP549Ra21 无翅型MMTV整合位点家族成员5A(WNT5A)单克隆抗体 WB; IHC; ICC; IP.
SEP549Ra 无翅型MMTV整合位点家族成员5A(WNT5A)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMP549Ra 无翅型MMTV整合位点家族成员5A(WNT5A)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSP549Ra01 无翅型MMTV整合位点家族成员5A(WNT5A)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Liver International Secreted frizzled-related protein 5 (Sfrp5) decreases hepatic stellate cell activation and liver fibrosis [Pubmed:25488180]
Journal of Physiology and Biochemistry Wnt5a reverses the inhibitory effect of hyperoxia on transdifferentiation of alveolar epithelial type II cells to type I cells [PubMed: 26547443]
Liver Secreted frizzled‐related protein 5 (Sfrp5) decreases hepatic stellate cell activation and liver fibrosis [PubMed: 25488180]
Transl Res Wnt5a is expressed in spondyloarthritis and exerts opposite effects on enthesis and bone in murine organ and cell cultures [PubMed: 26163991]
不确定杂志名 Untersuchungen der Serum-Konzentrationen des proinflammatorischen Glykoproteins wnt-5a und seines antiinflammatorischen Inhibitors sFRP-5 bei an … [servlets:MCRFileNodeServlet]
Life Sciences Hepatoprotective effect of hesperidin in hepatocellular carcinoma: Involvement of Wnt signaling pathways. [pubmed:28754618]
Arteriosclerosis, Thrombosis, and Vascular Biology  Effect of SFRP5 (Secreted Frizzled–Related Protein 5) on the WNT5A (Wingless-Type Family Member 5A)-Induced Endothelial Dysfunction and Its Relevance With … [Pubmed:29674475]
Stem Cells Reduced Cell Division Control Protein 42 Activity Compromises Hematopoiesis‐Supportive Function of Fanconi Anemia Mesenchymal Stromal Cells [Pubmed:29377497]
Science Translational Medicine Adipose tissue–derived WNT5A regulates vascular redox signaling in obesity via USP17/RAC1-mediated activation of NADPH oxidases [Pubmed: 31534019]
J Clin Endocrinol Metab Role of wnt5a in Metabolic Inflammation in Humans [Pubmed: 30137542]
Cancer Research Host Wnt5a Potentiates Microenvironmental Regulation of Ovarian Cancer Metastasis [Pubmed: 31932454]
CLINICAL BIOCHEMISTRY Sfrp5/Wnt5a and Leptin/adiponectin levels in the serum and the periarterial adipose tissue of patients with peripheral arterial occlusive disease [Pubmed: 33188773]
Sao Paulo Medical Journal Association of serum levels of secreted frizzled-related protein 5 and Wnt member 5a with glomerular filtration rate in patients with type 2 diabetes mellitus and … [Pubmed: 32491086]
Biomaterials An improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties [34823885]
PLoS One Association of circulating MR-proADM with all-cause and cardiovascular mortality in the general population: Results from the KORA F4 cohort study [Pubmed:34990470]
J Clin Periodontol Association between bone turnover markers and periodontitis: A population‐based cross‐sectional study [Pubmed:35569034]
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