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血管紧张素转化酶2(ACE2)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Angiotensin I Converting Enzyme 2 (ACE2)

ACEH; ACEII; ACE-II; Peptidyl-Dipeptidase A; ACE-related carboxypeptidase; Angiotensin-converting enzyme homolog; Metalloprotease MPROT15

  • 血管紧张素转化酶2(ACE2)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 血管紧张素转化酶2(ACE2)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 血管紧张素转化酶2(ACE2)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEB886Mu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测血管紧张素转化酶2(ACE2),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的血管紧张素转化酶2(ACE2)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 81-89 85
EDTA plasma(n=5) 87-95 91
heparin plasma(n=5) 80-97 86

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的血管紧张素转化酶2(ACE2),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中血管紧张素转化酶2(ACE2)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 80-99% 90-99% 90-104% 83-92%
EDTA plasma(n=5) 85-103% 79-92% 96-105% 82-102%
heparin plasma(n=5) 89-103% 78-88% 79-101% 79-102%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将血管紧张素转化酶2(ACE2)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的血管紧张素转化酶2(ACE2)与连接于固相载体上的抗体结合,然后加入生物素化的血管紧张素转化酶2(ACE2)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的血管紧张素转化酶2(ACE2)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
EPB886Mu61 血管紧张素转化酶2(ACE2)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB886Mu61 血管紧张素转化酶2(ACE2)活性蛋白 Cell culture; Activity Assays.
APB886Mu01 血管紧张素转化酶2(ACE2)活性蛋白 Cell culture; Activity Assays.
RPB886Mu02 血管紧张素转化酶2(ACE2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB886Mu01 血管紧张素转化酶2(ACE2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB886Mu01 血管紧张素转化酶2(ACE2)多克隆抗体 WB; IHC; ICC; IP.
PAB886Mu02 血管紧张素转化酶2(ACE2)多克隆抗体 WB; IHC; ICC; IP.
PAB886Mu06 血管紧张素转化酶2(ACE2)多克隆抗体 WB; IHC; ICC; IP.
LAB886Mu81 血管紧张素转化酶2(ACE2)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAB886Mu71 血管紧张素转化酶2(ACE2)多克隆抗体(生物素标记) WB; IHC; ICC.
SEB886Mu 血管紧张素转化酶2(ACE2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB886Mu 血管紧张素转化酶2(ACE2)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Nature Medicine Angiotensin-Converting Enzyme 2 Overexpression Remarkably Ameliorated Glomerular Injury in a Rat Model of Diabetic Nephropathy: A Comparison with ACE Inhibition [MolMed: 10_11_liu]
Journal of Cancer Therapy Plasma Levels of Angiotensin-Converting Enzymes 1 and 2 and AGTR2 (T1247G and A5235G) Gene Polymorphisms Are Associated to Breast Cancer Progression. [Scirp: Source]
Reprod Toxicol.? ACE2 activation by xanthenone prevents leptin-induced increases in blood pressure and proteinuria during pregnancy in Sprague-Dawley rats [Pubmed:25205467]
Intensive Care Medicine Experimental Angiotensin converting enzymes in patients with acute respiratory distress syndrome [Content: 3]
Journal of Chromatography A Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858]
Journal of Clinical Pharmacy and Therapeutics Relationship between genetic variants of ACE2 gene and circulating levels of ACE2 and its metabolites. [pubmed:28895159]
Innate Immunity  Lipoxin A attenuates LPS-induced acute lung injury via activation of the ACE2-Ang-(1-7)-Mas axis [Pubmed:29969931]
Journal of Surgical Research Impact of angiotensin-converting enzyme 2 levels on postoperative pneumonia after esophagectomy [Pubmed:29506841]
canadian journal of physiology and pharmacology Could cardioprotective effect of ACE2 activator “diminazene aceturate” is more potent than ACE inhibitor “Enalapril” on acute myocardial infarction in rats? [Pubmed: 30840489]
Intensive Care Medicine Angiotensin-converting enzymes in acute respiratory distress syndrome []
BioMed Research International AVE 0991 Attenuates Pyroptosis and Liver Damage after Heatstroke by Inhibiting the ROS-NLRP3 Inflammatory Signalling Pathway []
Heart and vessels Relationship between circulating levels of angiotensin-converting enzyme 2-angiotensin-(1–7)-MAS axis and coronary heart disease [Pubmed: 31359146]
Medicine Alteration and association between serum ACE2/angiotensin (1-7)/Mas axis and oxidative stress in chronic kidney disease: A pilot study [Pubmed: 32756181]
American Journal of Respiratory and Critical Care Medicine Equilibrium Angiotensin Metabolite Profiling in Patients with Acute Respiratory Distress Syndrome Indicates Angiotensin Converting Enzyme Inhibition [Pubmed: 32628511]
Clinical Research in Cardiology Determinants of soluble angiotensin-converting enzyme 2 concentrations in adult patients with complex congenital heart disease [33280062]
Clinical & Translational Immunology Pattern of circulating SARS©\CoV©\2©\specific antibody©\secreting and memory B©\cell generation in patients with acute COVID©\19 [33552508]
researchsquare Novel transgenic mice with Cre-dependent co-expression of GFP and human ACE2: a safe tool for study of COVID-19 pathogenesis [33855640]
Viruses Different Neutralization Sensitivity of SARS-CoV-2 Cell-to-Cell and Cell-Free Modes of Infection to Convalescent Sera []
Sci Rep Immunoreactivity of the SARS-CoV-2 entry proteins ACE-2 and TMPRSS-2 in murine models of hormonal manipulation, ageing, and cardiac injury [34907257]
European Journal of Clinical Investigation Angiotensin‐converting enzyme 2 and Transmembrane protease serine 2 in female and male patients with end‐stage kidney disease [Pubmed:35366343]
Metabolites Ramipril Reduces Acylcarnitines and Distinctly Increases Angiotensin-Converting Enzyme 2 Expression in Lungs of Rats [Pubmed:35448480]
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