抗膜联蛋白A1抗体(Anti-ANXA1)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Anti-Annexin A1 Antibody (Anti-ANXA1)
ANX-A1; ANX1; LPC1; Lipocortin I; Chromobindin-9; Calpactin II; Phospholipase A2 inhibitory protein
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测抗膜联蛋白A1抗体(Anti-ANXA1)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的抗膜联蛋白A1抗体(Anti-ANXA1)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 95-104 | 99 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的抗膜联蛋白A1抗体(Anti-ANXA1)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中抗膜联蛋白A1抗体(Anti-ANXA1)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 96-103% | 79-93% | 89-102% | 96-104% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100μL，37°C孵育1小时；
4. 洗板5次；
5. 加TMB底物90µL，37°C孵育10-20分钟；
6. 加终止液50µL，立即450nm读数。
实验原理
将抗原包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的抗膜联蛋白A1抗体(Anti-ANXA1)与连接于固相载体上的抗原结合，然后加入HRP标记的二抗，彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的抗膜联蛋白A1抗体(Anti-ANXA1)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| APE787Hu01 | 膜联蛋白A1(ANXA1)活性蛋白 | Cell culture; Activity Assays. |
| RPE787Hu01 | 膜联蛋白A1(ANXA1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAE787Hu01 | 膜联蛋白A1(ANXA1)多克隆抗体 | WB; IHC; ICC; IP. |
| PAE787Hu02 | 膜联蛋白A1(ANXA1)多克隆抗体 | WB; IHC; ICC; IP. |
| LAE787Hu81 | 膜联蛋白A1(ANXA1)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAE787Hu71 | 膜联蛋白A1(ANXA1)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAE787Hu22 | 膜联蛋白A1(ANXA1)单克隆抗体 | WB |
| MAE787Hu21 | 膜联蛋白A1(ANXA1)单克隆抗体 | WB; IHC; ICC; IP. |
| SEE787Hu | 膜联蛋白A1(ANXA1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| AEE787Hu | 抗膜联蛋白A1抗体(Anti-ANXA1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| SCE787Hu | 膜联蛋白A1(ANXA1)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LME787Hu | 膜联蛋白A1(ANXA1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSE787Hu01 | 膜联蛋白A1(ANXA1)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Plos one | Dysregulation of Anti-Inflammatory Annexin A1 Expression in Progressive Crohns Disease [PubMed: PMC3794972] |
| African Journals Online | Modulatory Effect of Phytoestrogens and Curcumin on Induction of Annexin 1 in Human Peripheral Blood Mononuclear Cells and their Inhibitory Effect on Secretory Phospholipase A2 [Ajol: Source] |
| Antimicrobial Agents and Chemotherapy | Host Biomarkers of Invasive Pulmonary Aspergillosis to Monitor Therapeutic Response [Asm: Source] |
| J Proteomics | Characterization of secretomes from a human blood brain barrier endothelial cells in-vitro model after ischemia by stable isotope labeling with aminoacids in cell culture (SILAC) [PubMed: 26718731] |
| Diabetes | Annexin A1 Is a Key Modulator of Mesenchymal Stromal Cell–Mediated Improvements in Islet Function [PubMed: 26470781] |
| PLoS One | Annexin A1 Is Increased in the Plasma of Preeclamptic Women [PubMed: 26398190] |
| 中国现代医学杂志 China Journal of Modern Medicine | 膜联蛋白A1 和CA19-9 在胆管癌中的表达及其临床意义 [article:8686] |
| arteriosclerosis thrombosis and vascular biology | Protective Aptitude of Annexin A1 in Arterial Neointima Formation in Atherosclerosis-Prone Mice-Brief Report. [pubmed:28062503] |
| Clinical Science | Mesenchymal stromal cells improve human islet function through released products and extracellular matrix. [pubmed:29101297] |
| Biomedicine & Pharmacotherapy | Proresolving protein Annexin A1: The role in type 2 diabetes mellitus and obesity [Pubmed:29677533] |
| International Journal of Chronic Obstructive Pulmonary Disease | Annexin A1 is elevated in patients with COPD and affects lung fibroblast function [Pubmed:29440885] |
| Medical Mycology | Applying host disease status biomarkers to therapeutic response monitoring in invasive aspergillosis patients [Pubmed:29370415] |
| DEVELOPMENTAL NEUROPSYCHOLOGY | EFFECTS OF MULTICOMPONENT EXERCISE ON PROGRESSION OF BRAIN ATROPHY AND COGNITION IN AMNESTIC MILD COGNITIVE IMPAIRMENT … [pdf:3395] |
| Multiple sclerosis and related disorders | Resolution of inflammation in neuromyelitis optica spectrum disorders [Pubmed: 30300851] |
| Acta Pharmaceutica | Effects of annexin A1 on apoptosis and cell cycle arrest in human leukemic cell lines [] |
| npj Science of Food | Annexin A1 accounts for an anti-inflammatory binding target of sesamin metabolites [Pubmed: 32133417] |
| FASEB JOURNAL | ANXA1 directs Schwann cells proliferation and migration to accelerate nerve regeneration through the FPR2/AMPK pathway [Pubmed: 32908567] |
| Rev Soc Bras Med Trop | Expression of annexin-A1 in blood and tissue leukocytes of leprosy patients [33263684] |
| science advances | Annexin-A1 SUMOylation regulates microglial polarization after cerebral ischemia by modulating IKK¦Á stability via selective autophagy [33523920] |
