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骨成型蛋白2(BMP2)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Bone Morphogenetic Protein 2 (BMP2)

BMP2A; BMP-2A; Hemochromatosis Modifier

  • 骨成型蛋白2(BMP2)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 骨成型蛋白2(BMP2)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 骨成型蛋白2(BMP2)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA013Bo.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测骨成型蛋白2(BMP2)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。

实验原理

将骨成型蛋白2(BMP2)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的骨成型蛋白2(BMP2)与连接于固相载体上的抗体结合，然后加入生物素化的骨成型蛋白2(BMP2)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的骨成型蛋白2(BMP2)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。

相关产品

编号 适用物种：Bos taurus; Bovine (Cattle，牛) 应用(仅供研究使用，不用于临床诊断！)
RPA013Bo01 骨成型蛋白2(BMP2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA013Bo01 骨成型蛋白2(BMP2)多克隆抗体 WB; IHC; ICC; IP.
MAA013Bo22 骨成型蛋白2(BMP2)单克隆抗体 WB; IHC; ICC; IP.
MAA013Bo21 骨成型蛋白2(BMP2)单克隆抗体 WB; IHC; ICC; IP.
SEA013Bo 骨成型蛋白2(BMP2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA013Bo 骨成型蛋白2(BMP2)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
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Medical Journal of Chinese People's Liberation Army Effect of transplantation of BMP-2-induced bone marrow mesenchymal stem cells on myocardial infarction of rats after reperfusion [Source]
PLOS ONE PDGF-AA promotes osteogenic differentiation and migration of mesenchymal stem cell by down-regulating PDGFRα and derepressing BMP-Smad1/5/8 signaling [Pubmed:25470749]
Eur J Pharmacol Negative effect of serotonin–norepinephrine reuptake inhibitor therapy on rat bone tissue after orchidectomy [PubMed: 25934570]
Pharmacology Effect of Mirtazapine on Rat Bone Tissue after Orchidectomy [PubMed: 25871861]
Journal of Cellular Biochemistry Titanium with Nanotopography Induces Osteoblast Differentiation by Regulating Endogenous Bone Morphogenetic Protein Expression and Signaling Pathway [PubMed: 26681207]
International Journal of Nanomedicine Sustained dual release of placental growth factor-2 and bone morphogenic protein-2 from heparin-based nanocomplexes for direct osteogenesis [Pubmed:27042064]
Research report EFFECTS OF FLUORIDE ON THE EXPRESSION OF BMP-2 AND SMAD1 IN RAT OSTEOBLASTS IN VITRO [files:p013-022]
Gene  Unveiling the interactions among BMPR-2, ALK-1 and 5-HTT genes in the pathophysiology of HAPE [Pubmed:27196063]
Journal of Materials Chemistry B Extracellular Matrix Powder from Cultured Cartilage-Like Tissue as Cell Carriers for Cartilage Repair [DOI:10.1039/C7TB00640C]
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Pharmacology Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive Rats [Pubmed:29898457]
Journal of Applied Biomaterials & Functional Materials BMP-2 and type I collagen preservation in human deciduous teeth after demineralization [Pubmed:30045659]
Applied Surface Science Enhanced osteogenic differentiation of MC3T3-E1 on rhBMP-2 immobilized titanium surface through polymer-mediated electrostatic interaction []
Materials Science and Engineering: C Injectable hydrogels from enzyme-catalyzed crosslinking as BMSCs-laden scaffold for bone repair and regeneration []
Biomaterials Science Development of CaCO 3 microsphere-based composite hydrogel for dual delivery of growth factor and Ca to enhance bone regeneration []
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY The dose-time effects of fluoride on the expression and DNA methylation level of the promoter region of BMP-2 and BMP-7 in rats [Pubmed: 32004919]
Journal of Industrial and Engineering Chemistry Biofabrication and application of decellularized bone extracellular matrix for effective bone regeneration []
BMC Musculoskeletal Disorders Synergistic Effect of Platelet-rich Fibrin Releasate and Bone Marrow Stem Cells in Preventing Bone Loss in Ovariectomized Mouse []
Bioact Mater New design to remove leukocytes from platelet-rich plasma (PRP) based on cell dimension rather than density [33842739]
Tissue Eng Regen Med Osteogenic and Angiogenic Potency of VEGF165-Transfected Canine Bone Marrow Mesenchymal Cells Combined with Coral Hydroxyapatite in Vitro [34302695]
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ACS Biomater Sci Eng Polydopamine-Coated Poly(l-lactide) Nanofibers with Controlled Release of VEGF and BMP-2 as a Regenerative Periosteum [34472855]
Phytomedicine Efficacy of Total flavonoids of Rhizoma drynariae on the Blood Vessels and the Growth Quality of Bone Graft in the Induced Membrane [Pubmed:35278899]
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