太阳集团tyc5997_平台(china)官方网站

补体成分3(C3)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Complement Component 3 (C3)

ASP; CPAMD1; Complement Protein C3; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3a anaphylatoxin; Acylation stimulating protein

  • 补体成分3(C3)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 补体成分3(C3)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 补体成分3(C3)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA861Bo.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测补体成分3(C3)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的补体成分3(C3)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 83-95 91
EDTA plasma(n=5) 95-104 98
heparin plasma(n=5) 98-105 102

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的补体成分3(C3)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中补体成分3(C3)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 98-105% 79-104% 90-98% 88-101%
EDTA plasma(n=5) 80-101% 95-105% 94-101% 90-104%
heparin plasma(n=5) 83-101% 78-101% 97-105% 98-105%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）50µL，
    加入50µL检测液A(临用前配制);
    37°C温育1小时。
3. 洗板3次；
4. 加检测溶液B100µL，37°C孵育30分钟；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分钟；
7. 加终止液50µL，立即450nm读数。

实验原理

本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将补体成分3(C3)单克隆抗体包被微孔板，制成固相载体，往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本)，待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物，然后加入HRP标记的亲和素，经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。待测标本浓度越高，标记抗原和抗体的结合就越受到抑制，显色愈浅。显色的深浅与酶量呈正相关，而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度（O.D.值），计算样品浓度。

相关产品

编号 适用物种：Bos taurus; Bovine (Cattle，牛) 应用(仅供研究使用，不用于临床诊断！)
RPA861Bo01 补体成分3(C3)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA861Bo01 补体成分3(C3)多克隆抗体 WB; IHC; ICC; IP.
SEA861Bo 补体成分3(C3)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA861Bo 补体成分3(C3)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Artificial Organs Experimental Research With Synthetic Copolymer-Coated Cardiopulmonary Bypass Circuits: Inflammatory and Thrombogenicity Analysis [Wiley: source]
Journal of Clinical Immunology Complement activation contributes to the injury and outcome of kidney in human anti-glomerular basement membrane disease. [PubMed: 22941511]
Lipids in Health and Disease Beneficial effects of omega-3 fatty acids in the proteome of high-density lipoprotein proteome [PubMed: PMC3480889]
Am J Reprod Immunol Human IgG Antinuclear Antibodies Induce Pregnancy Loss in Mice by Increasing Immune Complex Deposition in Placental Tissue: In Vivo Study [PubMed: 26388133]
Veterinary Immunology and Immunopathology Oral administration of heat-inactivated Mycobacterium bovis reduces the response of farmed red deer to avian and bovine tuberculin [Pubmed:27032499]
International Journal of Molecular Sciences Identification of Serum Peptidome Signatures of Non-Small Cell Lung Cancer [Pubmed:27043541]
Food Chem. Comparative proteomics of milk fat globule membrane in goat colostrum and mature milk [Pubmed:27173528]
Scientific Reports Generation of complement protein C3 deficient pigs by CRISPR/Cas9-mediated gene targeting. [pubmed:28694465]
Veterinary Microbiology The response of red deer to oral administration of heat-inactivated Mycobacterium bovis and challenge with a field strain. [pubmed:28888638]
Proteomics Clinical Applications iTRAQ‐Based Proteomic Analysis Reveals Protein Profile in Plasma from Children with Autism [Pubmed:29274201]
Nanomaterials An OMV-Based Nanovaccine Confers Safety and Protection against Pathogenic Escherichia coli via Both Humoral and Predominantly Th1 Immune Responses in … [Pubmed: 33233490]
Diagnostics Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2J [Pubmed: 32585848]
Scientific Reports The bovine cumulus proteome is influenced by maturation condition and maturational competence of the oocyte [Pubmed: 32555221]
Int Immunopharmacol Complement component 3 prevents imiquimod-induced psoriatic skin inflammation by inhibiting apoptosis in mice [Pubmed: 32535539]
Italian Journal of Animal Science Effects of chitooligosaccharide supplementation on laying performance, egg quality, blood biochemistry, antioxidant capacity and immunity of laying hens during the … []
Dev Comp Immunol A deep-sea pathogenic Bacillus subtilis isolate employs different strategies to escape the killing of teleost and murine complements [33545212]
Animal Effect of alfalfa hay and starter feed supplementation on caecal microbiota and fermentation, growth, and health of yak calves [33610512]
British Journal of Pharmacology The suppression of Brd4 inhibits peripheral plasma cell differentiation and exhibits therapeutic potential for systemic lupus erythematosus []
Scientific Reports Maternal Serum Proteomic Profiles of Pregnant Women With Type 1 Diabetes [34940989]
留言咨询
Baidu
sogou