内皮素1(EDN1)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Endothelin 1 (EDN1)
ET1; PPET1; Preproendothelin-1; Big endothelin-1
- 编号CEA482Po
- 物种Sus scrofa; Porcine (Pig，猪) 相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长2h
- 检测范围12.35-1,000pg/mL
- 灵敏度最小可检测剂量小于等于4.74pg/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 3175 ¥ 4536 ¥ 20412 ¥ 38556 ¥ 317520
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
-
产品包装(模拟)
-
产品包装(模拟)
-
实验结果图
-
标准曲线图
-
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测内皮素1(EDN1)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的内皮素1(EDN1)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 83-97 | 91 |
| EDTA plasma(n=5) | 92-105 | 96 |
| heparin plasma(n=5) | 86-94 | 90 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的内皮素1(EDN1)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中内皮素1(EDN1)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 85-96% | 92-103% | 95-103% | 92-101% |
| EDTA plasma(n=5) | 83-105% | 78-93% | 82-91% | 84-95% |
| heparin plasma(n=5) | 99-105% | 95-105% | 90-98% | 85-94% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）50µL，
加入50µL检测液A(临用前配制);
37°C温育1小时。
3. 洗板3次；
4. 加检测溶液B100µL，37°C孵育30分钟；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分钟；
7. 加终止液50µL，立即450nm读数。
实验原理
本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将内皮素1(EDN1)单克隆抗体包被微孔板，制成固相载体，往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本)，待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物，然后加入HRP标记的亲和素，经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。待测标本浓度越高，标记抗原和抗体的结合就越受到抑制，显色愈浅。显色的深浅与酶量呈正相关，而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度（O.D.值），计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Sus scrofa; Porcine (Pig，猪) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA482Po01 | 内皮素1(EDN1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPA482Po11 | 内皮素1(EDN1)牛血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| PAA482Po08 | 内皮素1(EDN1)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA482Po01 | 内皮素1(EDN1)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA482Po81 | 内皮素1(EDN1)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAA482Po71 | 内皮素1(EDN1)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAA482Po21 | 内皮素1(EDN1)单克隆抗体 | WB; IHC; ICC; IP. |
| CEA482Po | 内皮素1(EDN1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA482Po | 内皮素1(EDN1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Heart and Circulatory Physiology | B-type natriuretic peptide, vascular endothelial growth factor, endothelin-1, and nitric oxide synthase in chronic mountain sickness [APS: source] |
| PLOS ONE | CD98 Positive Eosinophils Contribute to T Helper 1 Pattern Inflammation [PlosOne: Source] |
| 36 | Megakaryocytic leukemia 1 (MKL1) ties the epigenetic machinery to hypoxia-induced transactivation of endothelin-1 [PubMed: PMC3695508] |
| Clinics (Sao Paulo). | Antifibrotic effects of crocetin in scleroderma fibroblasts and in bleomycin-induced sclerotic mice [Pubmed: 24212843] |
| Life Sci. | Attenuation of renal ischemia/reperfusion injury by a?aí extract preconditioning in a rat model [Pubmed:25476829] |
| Transpl Int. | One or 4 h of “in‐house” reconditioning by machine perfusion after cold storage improve reperfusion parameters in porcine kidneys [Pubmed:24977654] |
| J Endocrinol Invest.? | The interplay among iron metabolism, endothelium and inflammatory cascade in dysmetabolic disorders [Pubmed:25245337] |
| Chemical Research in Toxicology | Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal Instillation [Pubmed:25580880] |
| Journal of Surgical Research | Pivotal role of mast cell carboxypeptidase A in mediating protection against small intestinal ischemia–reperfusion injury in rats after ischemic preconditioning [Sciencedirect:S0022480414004909] |
| Pharmaceutical Biology | The promising effect of barberry (Zereshk) extract against experimental pulmonary microvascular remodeling and hypertension: A comparison with sildenafil [PubMed: 26023989] |
| A Periodical of the Hungarian Academy of Sciences | The effect of high altitude on endothelial and vascular dysfunction markers in preeclamptic patients [PubMed: 26690031] |
| Chemical Research in Toxicology | Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal Instillation [PubMed: 25580880] |
| Human & Experimental Toxicology | An in vivo and in vitro study High-dosage Danshen injection induces peripheral vascular endothelial cells injury [PubMed: 26078283] |
| Artery Research | Endothelin 1 and endothelial dysfunction in children with idiopathic nephrotic syndrome [science:article] |
| Transplant proc | Cardiovascular Risk Factors and Markers of Atherosclerosis in Stable Kidney TransplantRecipients. [pubmed:27496444] |
| Biomed Journal | Association of endothelial dysfunction and cytotoxin-associated gene A-positive Helicobacter pylori in patients with cardiac syndrome X. [pubmed:27884380] |
| International Journal of Advanced Research in Biological Sciences | Comparative studies on lens structure and function of newborn rats maternally treated with aspartame, monosodium glutamate and galactose [pdfcopy:42552] |
| Journal of Drug Metabolisn& Toxicology | Abnormal Retinal Structure and Function of Mother Wistar Rats Supplemented Aspartame, Glutamate and Galactose [587e5b6508aed3826af45e2d.pdf] |
| Polish Journal of Veterinary Sciences | Antioxidant and anti-inflammatory activities of a commercial noni juice revealed by carrageenan-induced paw edema [pubmed:27760029] |
| Artery Research | Endothelin 1 and endothelial dysfunction in children with idiopathic nephrotic syndrome [article:S1872-9312(16)30085-0] |
| Journal of Molecular Biomarkers J & Diagnosis | Biochemical and Molecular Markers of Congenital and Senile Cataractous Lenses [DOI: 10.4172/2155-9929.1000318] |
| A Study of Effect of Experimental Pre-Eclampsia on Plasma Lipocalin-2 Level in Rats. [AN=123819331] | |
| Journal of Functional Foods | Antihypertensive effects of Ocimum gratissimumextract: Angiotensin-converting enzyme inhibitor in vitro and in vivo investigation [10.1016/j.jff.2017.05.033] |
| Radiation Research | Selective Insulin-like Growth Factor Resistance Associated with Heart Hemorrhages and Poor Prognosis in a Novel Preclinical Model of the Hematopoietic Acute … [Pubmed:29809108] |
| Scandinavian Journal of Clinical and Laboratory Investigation | Isolated kidney perfusion: the influence of pulsatile flow [Pubmed:29301417] |
| Endokrynologia Polska | Endothelin-1 (ET-1), N-terminal fragment of proatrial natriuretic peptide (NTpro-ANP) and tumor necrosis factor alpha (TNF-α) in children with primary hypertension … [Pubmed: 30359461] |
| Journal of Pharmacological Sciences | Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndrome [Pubmed: 30342783] |
| Molecular Nutrition & Food Research | Short‐Term Isocaloric Intake of a Fructose‐but not Glucose‐Rich Diet Affects Bacterial Endotoxin Concentrations and Markers of Metabolic Health in Normal Weight … [Pubmed: 30570214] |
| Journal of Cosmetic Dermatology | Whitening effects of cosmetic formulation in the vascular component of skin pigmentation [Pubmed: 31074159] |
| Eurasian Journal of Clinical Sciences | APASL Single Topic Conference DELTA HEPATITIS, 27-28 June 2019, Baku, Azerbaijan [] |
| Journal of Functional Foods | Potent anti-inflammatory activity of polysaccharides extracted from Blidingia minima and their effect in a mouse model of inflammatory bowel disease [] |
| Scientific Reports | Integrated metabolomics and gut microbiome to the effects and mechanisms of naoxintong capsule on type 2 diabetes in rats [Pubmed: 32616735] |
| Evidence-Based Complementary and Alternative Medicine | Tianma Gouteng Decoction Exerts Cardiovascular Protection by Upregulating OPG and TRAIL in Spontaneously Hypertensive Rats [Pubmed: 33133215] |
| Microvasc Res | Effect of liraglutide on microcirculation in rat model with absolute insulin deficiency [34119534] |
| Biomed Pharmacother | Tianma Gouteng Decoction regulates oxidative stress and inflammation in AngII-induced hypertensive mice via transcription factor EB to exert anti … [34736077] |
| J Ethnopharmacol | Bai-Hu-Tang regulates endothelin-1 and its signalling pathway in vascular endothelial cells [34752899] |
| J Ethnopharmacol | Post-stroke treatment of storax improves long-term outcomes of stroke in rats [34343648] |
| Optical Technologies for Biology and Medicine | Laser Doppler flowmetry in assessing the effect of liraglutide on microcirculation in rats with absolute insulin deficiency [] |
留言咨询