G蛋白偶联雌激素受体1(GPER)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for G Protein Coupled Estrogen Receptor 1 (GPER)
GPR30; CMKRL2; DRY12; FEG-1; GPCR-Br; LERGU; LyGPR; Membrane estrogen receptor; Chemoattractant receptor-like 2; Flow-induced endothelial G-protein coupled receptor 1
- 编号SEG045Mu
- 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围0.312-20ng/mL
- 灵敏度最小可检测剂量小于等于0.114ng/mL.
- 样本类型Tissue homogenates, cell lysates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 3024 ¥ 4320 ¥ 19440 ¥ 36720 ¥ 302400
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测G蛋白偶联雌激素受体1(GPER)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将G蛋白偶联雌激素受体1(GPER)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的G蛋白偶联雌激素受体1(GPER)与连接于固相载体上的抗体结合，然后加入生物素化的G蛋白偶联雌激素受体1(GPER)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的G蛋白偶联雌激素受体1(GPER)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPG045Mu01 | G蛋白偶联雌激素受体1(GPER)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPG045Mu02 | G蛋白偶联雌激素受体1(GPER)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAG045Mu01 | G蛋白偶联雌激素受体1(GPER)多克隆抗体 | WB; IHC; ICC; IP. |
| PAG045Mu02 | G蛋白偶联雌激素受体1(GPER)多克隆抗体 | WB; IHC; ICC; IP. |
| SEG045Mu | G蛋白偶联雌激素受体1(GPER)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMG045Mu | G蛋白偶联雌激素受体1(GPER)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| The Journal of Steroid Biochemistry and Molecular Biology | Neuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR30 signalling [Pubmed:24846829] |
| Molecular and Cellular Endocrinology | Isomer-nonspecific action of dichlorodiphenyltrichloroethane on aryl hydrocarbon receptor and G-protein-coupled receptor 30 intracellular signaling in apoptotic neuronal cells [Pubmed:24859647] |
| J Steroid Biochem Mol Biol | Neuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR32 signalling [PubMed: 24846831] |
| Journal of Environmental Sciences | Bisphenol A exposure alters release of immune and developmental modulators and expression of estrogen receptors in human fetal lung fibroblasts [science:S1001074216300924] |
| Psychiatry research | Serum levels of G protein-coupled estrogen receptor 1 (GPER1) in drug-naive patients with generalized anxiety disorder. [pubmed:27512921] |
| Clinical Psychopharmacology Neuroscience. | Increased Serum G Protein-coupled Estrogen Receptor 1 Levels and Its Diagnostic Value in Drug Naïve Patients with Major Depressive Disorder [pubmed:29073745] |
| Psychiatry Research | Decreased levels of G protein-coupled estrogen receptor in children with autism spectrum disorders. [pubmed:28734238] |
| Neuropsychiatric disease and treatment | serum levels of gPer-1 in euthymic bipolar patients [Pubmed:29618927] |
| European archives of oto-rhino-laryngology | An evaluation of G-protein coupled membrane estrogen receptor-1 level in stuttering [Pubmed:29299745] |
| Bosnian Journal of Basic Medical Sciences | Evaluation of estrogen and G protein-coupled estrogen receptor 1 (GPER) levels in drug-naïve patients with attention deficit hyperactivity disorder (ADHD) [Pubmed:29659348] |
| Carleton University Research Virtual Environment | In Vitro Study of Mechanisms Underlying the Developmental Effects of Bisphenol A Using Human Fetal Lung Fibroblasts [] |
| Am J Otolaryngol | GPER-1 and sex-hormone levels in patients with otosclerosis [Pubmed: 32144019] |
| Andrologia. | Relationship between the G protein–coupled oestrogen receptor and spermatogenesis, and its correlation with male infertility [Pubmed: 32776559] |
| Journal of Radiation Research and Applied Sciences | GPER-1 in chronic hepatitis B [] |
| FASEB J | Endocrine disruption in Crohn's disease: Bisphenol A enhances systemic inflammatory response in patients with gut barrier translocation of dysbiotic microbiota?¡ [34085740] |
| Comparing the Impact of Serum GPER-1 and Oxidant/Antioxidant Levels on Retinopathy in Diabetic Patients and Healthy Individuals: A Pilot Study [] | |
| Horm Mol Biol Clin Investig | The change pattern in serum G protein-coupled estrogen receptor-1 (GPER1) levels during pregnancy with and without gestational diabetes mellitus [34787384] |
