窖蛋白(CAV1)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Caveolin 1 (CAV1)
VIP21; Caveolae Protein 22kDa
- 编号SEA214Ga
- 物种Chicken (Gallus，鸡) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围0.156-10ng/mL
- 灵敏度最小可检测剂量小于等于0.057ng/mL.
- 样本类型serum, plasma, tissue homogenates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2873 ¥ 4104 ¥ 18468 ¥ 34884 ¥ 287280
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测窖蛋白(CAV1)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的窖蛋白(CAV1)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 84-102 | 94 |
| EDTA plasma(n=5) | 84-94 | 90 |
| heparin plasma(n=5) | 88-98 | 94 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的窖蛋白(CAV1)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中窖蛋白(CAV1)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 98-105% | 83-94% | 92-99% | 98-105% |
| EDTA plasma(n=5) | 82-91% | 84-101% | 88-102% | 88-102% |
| heparin plasma(n=5) | 81-104% | 99-105% | 80-102% | 97-105% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将窖蛋白(CAV1)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的窖蛋白(CAV1)与连接于固相载体上的抗体结合，然后加入生物素化的窖蛋白(CAV1)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的窖蛋白(CAV1)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Chicken (Gallus，鸡) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA214Ga01 | 窖蛋白(CAV1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA214Ga01 | 窖蛋白(CAV1)多克隆抗体 | WB; IHC; ICC; IP. |
| MAA214Ga21 | 窖蛋白(CAV1)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA214Ga | 窖蛋白(CAV1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA214Ga | 窖蛋白(CAV1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Mendel Net | Novel prostate cancer tumour markers in a cell line model [Mendelu: source] |
| Oncology reports | Caveolin-1 as a potential high-risk prostate cancer biomarker [PubMed: 22159333] |
| Anticancer Research | Increased plasma caveolin-1 levels are associated with progression of prostate cancer among Japanese men [Pubmed: 23645736] |
| Sarcoidosis Vasc Diffuse Lung Dis.? | Decrased sputum caveolin-1 is associated with systemic sclerosis related lung disease [Pubmed:24751454] |
| Cancer Genomics and Proteomics | Significant Association of Caveolin-1 and Caveolin-2 with Prostate Cancer Progression [PubMed: 26543085] |
| Atherosclerosis | Genomic variant in CAV1 increases susceptibility to coronary artery disease and myocardial infarction [Pubmed:26775120] |
| Arthritis Research & Therapy | Increased caveolin-1 in intervertebral disc degeneration facilitates repair [Pubmed:26939667] |
| Oxid Med Cell Longev | Lower Serum Caveolin-1 Is Associated with Cerebral Microbleeds in Patients with Acute Ischemic Stroke [Pubmed:27119011] |
| Inflammation | Caveolin-1 Promotes the Imbalance of Th17/Treg in Patients with Chronic Obstructive PulmonaryDisease. [pubmed:27613621] |
| Oxidative Medicine and Cellular Longevity | Swim Training Modulates Skeletal Muscle Energy Metabolism, Oxidative Stress, and Mitochondrial Cholesterol Content in Amyotrophic Lateral Sclerosis Mice [Pubmed:29849903] |
| Microcirculation | Endothelium‐dependent hyperpolarization‐mediated vasodilatation compensates nitric oxide‐mediated endothelial dysfunction during ischemia in diabetes‐induced … [Pubmed:29665152] |
| Genetic Testing and Molecular Biomarkers | The CAV1 Gene 3′ Untranslated Region Single Nucleotide Polymorphisms Are Associated with the Risk of Pulmonary Hypertension in Chinese Han Chronic … [] |
| Cell Rep | SIRT6 transcriptionally regulates fatty acid transport by suppressing PPAR¦Ã [34077730] |
| researchsquare | Cav-1 Protein Levels in Serum and Infarcted Brain Correlate with Hemorrhagic Volume in a Mouse Model of Thromboembolic Stroke, Independently of Rt-PA?¡ [] |
| Molecules | 5, 2′-Dibromo-2, 4′, 5′-trihydroxydiphenylmethanone Inhibits LPS-Induced Vascular Inflammation by Targeting the Cav1 Protein [Pubmed:35566232] |
