结合珠蛋白(Hpt)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Haptoglobin (Hpt)
HP; Hp2-Alpha; Alpha-2-Macroglobulin; Zonulin
- 编号SEA817Ca
- 物种Canis familiaris; Canine (Dog，犬) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围3.12-200ng/mL
- 灵敏度最小可检测剂量小于等于1.23ng/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 3024 ¥ 4320 ¥ 19440 ¥ 36720 ¥ 302400
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测结合珠蛋白(Hpt)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的结合珠蛋白(Hpt)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 86-101 | 94 |
| EDTA plasma(n=5) | 80-89 | 86 |
| heparin plasma(n=5) | 78-104 | 97 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的结合珠蛋白(Hpt)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中结合珠蛋白(Hpt)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-89% | 93-101% | 85-97% | 84-98% |
| EDTA plasma(n=5) | 94-102% | 96-103% | 84-97% | 91-105% |
| heparin plasma(n=5) | 96-103% | 97-105% | 97-104% | 90-98% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将结合珠蛋白(Hpt)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的结合珠蛋白(Hpt)与连接于固相载体上的抗体结合，然后加入生物素化的结合珠蛋白(Hpt)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的结合珠蛋白(Hpt)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Canis familiaris; Canine (Dog，犬) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA817Ca01 | 结合珠蛋白(Hpt)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA817Ca01 | 结合珠蛋白(Hpt)多克隆抗体 | WB; IHC; ICC; IP. |
| MAA817Ca21 | 结合珠蛋白(Hpt)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA817Ca | 结合珠蛋白(Hpt)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA817Ca | 结合珠蛋白(Hpt)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Journal of Vascular Surgery | Circulation levels of acute phase proteins in patients with Takayasu arteritis [PubMed: 20100644] |
| Genetics | Quantitative trait loci mapping of the mouse plasma proteome (pQTL). [NCBI: PMC3567747] |
| ostaglandins Leukot Essent Fatty Acids. | Docosahexaenoic and eicosapentaenoic acid supplementation does not exacerbate oxidative stress or intravascular haemolysis in homozygous sickle cell patients. [Pubmed: 24095588] |
| PLoS One | Gut Microbial Dysbiosis May Predict Diarrhea and Fatigue in Patients Undergoing Pelvic Cancer Radiotherapy: A Pilot Study [PubMed: 25955845] |
| Journal of Chromatography A | Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858] |
| Annals of Neurology | High complement levels in astrocyte‐derived exosomes of Alzheimer disease [Pubmed:29406582] |
| European Journal of Medicinal Plants | Effects of Hippophae rhamnoides L. Leaf and Marc Extract with Reduced Tannin Concentration on the Health and Growth Parameters of Newborn Calves [] |
| Journal of Animal Science | Using ruminally protected and nonprotected active dried yeast as alternatives to antibiotics in finishing beef steers: growth performance, carcass traits, blood … [Pubmed: 30184125] |
| Journal of Clinical Medicine | Urinary Proteomics for the Early Diagnosis of Diabetic Nephropathy in Taiwanese Patients [Pubmed: 30486327] |
| International Journal of Biological Sciences | Generation of Pigs Resistant to Highly Pathogenic-Porcine Reproductive and Respiratory Syndrome Virus through Gene Editing of CD163 [] |
| Food and Chemical Toxicology | Prolyl endopeptidase-degraded low immunoreactive wheat flour attenuates immune responses in Caco-2 intestinal cells and gluten-sensitized BALB/c mice [Pubmed: 31082461] |
| Journal of Animal Science | Ruminally protected and unprotected Saccharomyces cerevisiae fermentation products as alternatives to antibiotics in finishing beef steers [Pubmed: 31410465] |
| International Journal of Biological Sciences | Deletion of CD163 Exon 7 Confers Resistance to Highly Pathogenic Porcine Reproductive and Respiratory Viruses on Pigs [] |
| Pediatric Obesity | Intestinal permeability and small intestine bacterial overgrowth in excess weight adolescents [Pubmed: 33089672] |
| JOURNAL OF ANIMAL SCIENCE | Processing Index of Barley Grain and Dietary Undigested Neutral Detergent Fiber Concentration Affected Chewing Behavior, Ruminal pH and Total Tract Nutrient?¡ [33474564] |
| large animal review | Influence of niacin application on inflammatory parameters, non-esterified fatty acids and functional status of liver in cows during early lactation [] |
| PLoS One | Could serum zonulin be an intestinal permeability marker in diabetes kidney disease? [34170951] |
| Probiotics Antimicrob Proteins | The Effect of Feed Supplementation with EM Bokashi® Multimicrobial Probiotic Preparation on Selected Parameters of Sow Colostrum and Milk as Indicators … [34596883] |
