热休克蛋白70(HSP70)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Heat Shock Protein 70 (HSP70)
DnaK; 70 Kilodalton Heat Shock Proteins
- 编号SEA873Po
- 物种Sus scrofa; Porcine (Pig，猪) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围3.12-200ng/mL
- 灵敏度最小可检测剂量小于等于1.16ng/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 3175 ¥ 4536 ¥ 20412 ¥ 38556 ¥ 317520
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测热休克蛋白70(HSP70)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的热休克蛋白70(HSP70)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 83-90 | 87 |
| EDTA plasma(n=5) | 81-98 | 87 |
| heparin plasma(n=5) | 86-93 | 90 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的热休克蛋白70(HSP70)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中热休克蛋白70(HSP70)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 85-101% | 79-103% | 95-104% | 78-101% |
| EDTA plasma(n=5) | 95-103% | 95-102% | 93-101% | 83-91% |
| heparin plasma(n=5) | 93-101% | 95-102% | 80-103% | 84-91% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将热休克蛋白70(HSP70)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的热休克蛋白70(HSP70)与连接于固相载体上的抗体结合，然后加入生物素化的热休克蛋白70(HSP70)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的热休克蛋白70(HSP70)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
相关产品
| 编号 | 适用物种：Sus scrofa; Porcine (Pig，猪) | 应用(仅供研究使用，不用于临床诊断！) |
| SEA873Po | 热休克蛋白70(HSP70)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Journal of Molecular Medicine | Proteomic analysis reveals significant elevation of heat shock protein 70 in patients with chronic heart failure due to arrhythmogenic right ventricular cardiomyopathy [SpringerLink: b8738j3571287425] |
| Journal of Neuroinflammation | Neuroinflammation in autism spectrum disorders [PubMed: PMC3549857] |
| Eur Arch Psychiatry Clin Neurosci. | Luteal serum BDNF and HSP70 levels in women with premenstrual dysphoric disorder [Pubmed: 23455589] |
| Arch Bronconeumol | Concentraciones de proteínas de estrés térmico, L-arginina y dimetilarginina asimétrica en pacientes con síndrome de apnea obstructiva del sue [PubMed: 26055465] |
| Arch Bronconeumol | Heat Shock Proteins, [PubMed: 26055465] |
| Animal Science Journal | The effect of heat stress on gene expression and synthesis of heat‐shock and milk proteins in bovine mammary epithelial cells [PubMed: 26467738] |
| Transplant Proc | L-Alanyl-Glutamine Attenuates Oxidative Stress in Liver Transplantation Patients [PubMed: 26518955] |
| Plos One | Long-Term Effects of Induced Hypothermia on Local and Systemic Inflammation-Results from a Porcine Long-Term Trauma Model [Pubmed:27144532] |
| J Pak Med Assoc. | Elevated interictal serum HSP-70 levels as an indicator of neurodegeneration for chronic migraine [mnstemps:33-1429777310] |
| Egyptian Poultry Science Journal | STUDY THE RELATIONSHIP BETWEEN SELENIUM AND HEAT SHOCK PROTEINS UNDER HEAT STRESS FOR LOCAL SINAI CHICKENS STRAIN [abstract:3d115259031] |
| Physiological consequences for rainbow trout (Oncorhynchus mykiss) of short-term exposure to increased suspended solid load [S0144860916301935] | |
| Animal Science Journal | Prokaryotic expression of chicken interferon-γ fusion protein and its effect on expression ofpoultry heat shock protein 70 under heat stress. [pubmed:27753175] |
| Pharmacol Rep | High-dose testosterone enanthate supplementation boosts oxidative stress, but exerts little effect on the antioxidant barrier in sedentary adolescent male rat liver. [pubmed:28531762] |
| Biomed Pharmacother. | SIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from death. [pubmed:28122311] |
| Immunobiology | Concentrations of SP-A and HSP70 are associated with polarization of macrophages in pleural effusions of non-small cell lung cancer [pubmed:29111316] |
| Egyptian Poultry Science Journal | EFFECT OF DIETARY SOURCE OF SELENIUM ON PRODUCTIVE AND REPRODUCTIVE PERFORMANCE OF SINAI LAYING HENS UNDER HEAT STRESS CONDITIONS. [125788660] |
| Biomedicine & Pharmacotherapy | SIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from death [DOI:10.1016/j.biopha.2017.01.087] |
| Egyptian Poultry Science Journal | IMPACT OF CHROMIUM PICOLINATE SUPPLEMENTATION ON PRODUCTIVE PERFORMANCE, IMMUNE RESPONSE AND HEAT SHOCK PROTEINS OF BROILER CHICKENS UNDER HEAT-STRESS CONDITION. [ISSN: 1110-5623] |
| Aquaculture | Physiological consequences of chronic exposure of rainbow trout (Oncorhynchus mykiss) to suspended solid load in recirculating aquaculture systems [10.1016/j.aquaculture.2017.11.030] |
| Journal of dairy research | Detection of 70 kDa heat shock protein in the saliva of dairy cows. [pubmed:28625184] |
| Journal of animal science | Reducing rumen starch fermentation of wheat with three percent sodium hydroxide has the potential to ameliorate the effect of heat stress in grain-fed wethers [pubmed:29293755] |
| Biochemistry and Cell Biology | Ectopic expression of Miro 1 ameliorates seizures and inhibits hippocampal neurodegeneration in a mouse model of pilocarpine epilepsy [Pubmed:29365285] |
| Egyptian Poultry Science Journal | THE EFFECT OF CHROMIUM PICOLINATE SUPPLEMENTATION ON IMMUNE RESPONSE AND HEAT SHOCK PROTEINS OF LAYING HENS UNDER HEAT … [article_5570_5c06907ac09a7724a58d2b796fe91aba.pdf] |
| Egyptian Poultry Science Journal | EFFECT OF MITIGATION OF HEAT STRESS BY EARLY HEATE ACCLIMATION AND GLUTAMINE INJECTION ON SOME PHYSIOLOGICAL MEASUREMENTS … [:] |
| FASEB Journal | Signature-oriented investigation of the efficacy of multicomponent drugs against heart failure [Pubmed: 30230922] |
| Journal of Biological Sciences | Maged Mohamed Abd-Elhakim Barakat, Noha Ahmed El-Boghdady, Ebtehal Kamal Eldin Farrag, Ataa Abdelhalim Said and Sylvia Edward Shaker [] |
| Biological Procedures Online | Effective Visualization and Easy Tracking of Extracellular Vesicles in Glioma Cells [] |
| Journal of Biological Sciences | Protective and Curative Effects of Bombax ceiba Flower and Ziziphus spina christi Fruit Extracts on Gastric Ulcer [] |
| 博士论文 | PHYSIOLOGICAL ADAPTATIONS TO HEAT ACCLIMATION; REPERCUSSIONS ON CYCLING PERFORMANCE [] |
| environmental science and pollution research | Possible prophylactic effect of omega-3 fatty acids on cadmium-induced neurotoxicity in rats' brains [Pubmed: 31468353] |
| ACTA TROPICA | Oxidative stress mediated apoptotic potential of mefloquine on experimental trichinellosis [Pubmed: 33221280] |
| BioMed Research International | Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress [Pubmed: 32685488] |
| Slovak Journal of Animal Science | EFFECTS OF HEAT STRESS ON PHYSIOLOGICAL PARAMETERS AND SERUM CONCENTRATION OF HSP70 IN INDIGENOUS BREEDS OF SHEEP IN NIGERIA [] |
| 博士论文 | Effect of organic acid blend and prebiotic on growth performance gut histomorphometry, tibia bone characteristics, reactive oxygen species, microbial count and … [] |
| Translational Medicine Communications | Concurrent high-intensity aerobic and resistance exercise modulates systemic release of alarmins (HMGB1, S100A8/A9, HSP70) and inflammatory … [] |
| Physiological impacts of suspended solids in recirculating aquaculture [] | |
| Clin Oral Investig | Evaluation of heat shock protein 70 and toll-like receptor 4 expression in gingival crevicular fluid in response to orthodontic forces [34091792] |
| Journal of Thermal Biology | Effects of L-proline on cellular responses of hen erythrocytes subjected to thermal stress [33627283] |
| J Korean Neurosurg Soc | The Restorative Effect of Gallic Acid on the Experimental Sciatic Nerve Damage Model [34376039] |
| PLoS One | Neuroprotective effect of sodium alginate against chromium-induced brain damage in rats [Pubmed:35421180] |
| Med Science | Identification of marker proteins in preterm and term birth: Relationship of rankl and HSP70 in the cord blood [] |
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